Anti-TRIM24 抗体 (ab70560)
Key features and details
- Rabbit polyclonal to TRIM24
- Suitable for: IHC-P, IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-TRIM24 antibody
TRIM24 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TRIM24 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, IP, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
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免疫原
Synthetic peptide within Human TRIM24 aa 700-750. The exact sequence is proprietary.
Database link: NP_056989.2 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70560の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/200.
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| IP |
Use at an assay dependent concentration.
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| WB |
Use a concentration of 0.1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 116 kDa).
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| 特記事項 |
|---|
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IHC-P
1/200. |
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IP
Use at an assay dependent concentration. |
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WB
Use a concentration of 0.1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 116 kDa). |
ターゲット情報
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機能
Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes. -
パスウェイ
Protein modification; protein ubiquitination. -
関連疾患
Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TRIM24/TIF1 is found in thyroid papillary carcinomas. Translocation t(7;10)(q32;q11) with RET. The translocation generates the TRIM24/RET (PTC6) oncogene. -
配列類似性
Contains 2 B box-type zinc fingers.
Contains 1 bromo domain.
Contains 1 PHD-type zinc finger.
Contains 1 RING-type zinc finger. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR.
Sumoylated. -
細胞内局在
Nucleus. Cytoplasm. Colocalizes with sites of active transcription. Detected both in nucleus and cytoplasm in some breast cancer samples. Predominantly nuclear. - Information by UniProt
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参照データベース
- Entrez Gene: 8805 Human
- Entrez Gene: 21848 Mouse
- Entrez Gene: 500084 Rat
- Omim: 603406 Human
- SwissProt: O15164 Human
- SwissProt: Q64127 Mouse
- Unigene: 490287 Human
- Unigene: 41063 Mouse
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別名
- E3 ubiquitin protein ligase TRIM24 antibody
- E3 ubiquitin-protein ligase Trim24 antibody
- hTIF1 antibody
see all
画像
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Western blot - Anti-TRIM24 antibody (ab70560)All lanes : Anti-TRIM24 antibody (ab70560) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 116 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM24 antibody (ab70560)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse teratoma tissue labelling TRIM24 with ab70560 at 1/200. Detection: DAB.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM24 antibody (ab70560)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling TRIM24 with ab70560 at 1/200. Detection: DAB.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab70560 は 8 報の論文で使用されています。
- Guo Y et al. Circ_RNF13 Regulates the Stemness and Chemosensitivity of Colorectal Cancer by Transcriptional Regulation of DDX27 Mediated by TRIM24 Stabilization. Cancers (Basel) 14:N/A (2022). PubMed: 36551703
- Xiao M et al. Long non-coding RNA ZFPM2-AS1 promotes colorectal cancer progression by sponging miR-137 to regulate TRIM24. Mol Med Rep 23:N/A (2021). PubMed: 33300060
- Mojsa B et al. Identification of SUMO Targets Associated With the Pluripotent State in Human Stem Cells. Mol Cell Proteomics 20:100164 (2021). PubMed: 34673284
- Chen M et al. NCK1-AS1 Increases Drug Resistance of Glioma Cells to Temozolomide by Modulating miR-137/TRIM24. Cancer Biother Radiopharm 35:101-108 (2020). PubMed: 31750728
- Xie H et al. Low let-7d exosomes from pulmonary vascular endothelial cells drive lung pericyte fibrosis through the TGFßRI/FoxM1/Smad/ß-catenin pathway. J Cell Mol Med 24:13913-13926 (2020). PubMed: 33179861
- Fyfe JJ et al. Enhanced skeletal muscle ribosome biogenesis, yet attenuated mTORC1 and ribosome biogenesis-related signalling, following short-term concurrent versus single-mode resistance training. Sci Rep 8:560 (2018). PubMed: 29330460
- Fang Z et al. TRIM24 promotes the aggression of gastric cancer via the Wnt/ß-catenin signaling pathway. Oncol Lett 13:1797-1806 (2017). WB, IHC-P ; Human . PubMed: 28454326
- Fang Z et al. Regulation of TRIM24 by miR-511 modulates cell proliferation in gastric cancer. J Exp Clin Cancer Res 36:17 (2017). WB . PubMed: 28114950