Anti-TRIM21/SS-A 抗体 (ab4369)
Key features and details
- Goat polyclonal to TRIM21/SS-A
- Suitable for: Flow Cyt (Intra), ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-TRIM21/SS-A antibody
TRIM21/SS-A 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to TRIM21/SS-A -
由来種
Goat -
アプリケーション
適用あり: Flow Cyt (Intra), ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to TRIM21/SS-A aa 463-475 (N terminal).
Sequence:CPLNIGSQGSTDY
(Peptide available asab23028) -
ポジティブ･コントロール
- ICC/IF: U2OS cells. Flow Cyt (intra): HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4369の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| 特記事項 |
|---|
|
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2. -
組織特異性
Isoforms 1 and 2 are expressed in fetal and adult heart and fetal lung. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger. -
ドメイン
The coiled-coil is necessary for the cytoplasmic localization. The B30.2/SPRY domain is necessary for the cytoplasmic localization, the interaction with IRF3 and for the IRF3-driven interferon beta promoter activity. The RING-type zinc finger is necessary for ubiquitination and for the IRF3-driven interferon beta promoter activity. Interacts with SKP2 and CUL1 in a RING finger-independent manner. -
翻訳後修飾
Autoubiquitinated; does not lead to its proteasomal degradation. Deubiquitinated by USP4; leading to its stabilization. -
細胞内局在
Cytoplasm. Nucleus. Cytoplasm > P-body. Enters the nucleus upon exposure to nitric oxide. Localizes to small dot- or rod-like structures in the cytoplasm, called cytoplasmic bodies (P-body) that are located underneath the plasma membrane and also diffusely in the cytoplasm and are highly motil in cells. Cytoplasmic bodies are located along the microtubules and do not share the same cytoplasmic bodies with TRIM5. Colocalizes with DCP2 in P-body. - Information by UniProt
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参照データベース
- Entrez Gene: 6737 Human
- Omim: 109092 Human
- SwissProt: P19474 Human
- Unigene: 532357 Human
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別名
- 52 kDa ribonucleoprotein autoantigen Ro/SS-A antibody
- 52 kDa Ro protein antibody
- 52kD Ro/SSA autoantigen antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-TRIM21/SS-A antibody (ab4369)
Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling TRIM21/SS-A with ab4369 at 10 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.
Negative control: Unimmunized goat IgG (10 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).
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Flow Cytometry (Intracellular) - Anti-TRIM21/SS-A antibody (ab4369)Flow cytometry analysis of HeLa cells labelling TRIM21/SS-A with ab4369 (blue line) at 10 µg/ml. Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton. Incubated with the primary antibody for 1 hour followed by incubation with Alexa Fluor® 488-conjugated secondary antibody at 1 µg/ml.
IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488-conjugated secondary antibody.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab4369 は 1 報の論文で使用されています。
- Kuboshima M et al. Presence of serum tripartite motif-containing 21 antibodies in patients with esophageal squamous cell carcinoma. Cancer Sci 97:380-6 (2006). PubMed: 16630135
