AB3942

Anti-TRIM2 抗体

Name: Anti-TRIM2 抗体 (ab3942) | Abcam
Brand: Abcam Limited
SKU: AB3942
Price: 74000 JPY
Availability: InStock

Anti-TRIM2 antibody

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(3 Publications)

Goat Polyclonal TRIM2 antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TRIM2 aa 700 to C-terminus.

別名を表示する

KIAA0517, RNF86, TRIM2, Tripartite motif-containing protein 2, E3 ubiquitin-protein ligase TRIM2, RING finger protein 86, RING-type E3 ubiquitin transferase TRIM2

1 Images

Unknown

Western blot - Anti-TRIM2 antibody (AB3942)

Primary incubation was for 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-TRIM2 antibody (ab3942) at 0.5 µg/mL

All lanes:

Rat brain tissue lysate at 35 µg

Predicted band size: 82 kDa

Observed band size: 30 kDa,80 kDa,85 kDa

false

Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Rat, Human

アプリケーション

IHC-P, WB

applications

免疫原

Synthetic Peptide within Human TRIM2 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9C040

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "6-12 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product.</p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

バッファー組成

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TRIM2 also known as tripartite motif-containing protein 2 is a protein that plays an essential role in protein ubiquitination. It has a molecular mass of approximately 75 kDa. TRIM2 is mainly found in neurons and has expression profiles in the brain and other neural tissues. The protein contains a RING-type zinc finger domain that facilitates its E3 ubiquitin ligase activity enabling it to tag specific proteins for degradation by the proteasome system.

Biological function summary

TRIM2 functions by modulating the stability of specific proteins involved in neuronal growth and maintenance. It operates as part of ubiquitination complexes allowing for targeted protein degradation. Neurodevelopmental processes rely on TRIM2 activity to maintain cellular homeostasis. Its involvement in axonal transport and stabilization of microtubule-associated proteins underlines its important role in nervous system integrity and functionality.

Pathways

TRIM2's function integrates into the ubiquitin-proteasome pathway and neural development processes. Within the ubiquitin-proteasome pathway TRIM2 interacts closely with other E3 ligases and substrates essential for proper cell functioning. It also relates to the Neurotrophin TRK receptor signaling pathway where TRIM2 can influence neuronal survival and differentiation. These pathways connect TRIM2 to proteins like TRK receptors and other ligases that manage protein turnover and neuronal signaling.

Disrupted TRIM2 function associates with neurological conditions such as Charcot-Marie-Tooth disease and neurodegenerative disorders. Mutations in TRIM2 lead to impaired ubiquitination activities contributing to these diseases' progression. TRIM2 dysfunction also links to abnormalities in proteins like neurofilament light chain which further aggravate disease pathology by destabilizing neuronal architectures and interrupting normal neural processes.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

UBE2D1-dependent E3 ubiquitin-protein ligase that mediates the ubiquitination of NEFL and of phosphorylated BCL2L11. Plays a neuroprotective function. May play a role in neuronal rapid ischemic tolerance. Plays a role in antiviral immunity and limits New World arenavirus infection independently of its ubiquitin ligase activity (PubMed : 24068738).

See full target information TRIM2

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39684673

2024

2'-Hydroxycinnamaldehyde, a Natural Product from Cinnamon, Alleviates Ischemia/Reperfusion-Induced Microvascular Dysfunction and Oxidative Damage in Rats by Upregulating Cytosolic BAG3 and Nrf2/HO-1.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Hsuan Cheng,Chih-Yao Chiang,Chung-Hsin Wu,Chiang-Ting Chien

International journal of molecular medicine 47: PubMed33760118

2021

Knockdown of long non‑coding RNA DLEU2 suppresses idiopathic pulmonary fibrosis by regulating the microRNA‑369‑3p/TRIM2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hengzhong Yi,Danlin Luo,Yangbao Xiao,Di Jiang

International journal of oncology 53:1643-1656 PubMed30066883

2018

TRIM2 regulates the development and metastasis of tumorous cells of osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Qin,Jichao Ye,Fulan Zhao,Shaoyu Hu,Suwei Wang

View all publications

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Abcam product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRIM2 抗体

Anti-TRIM2 antibody

Western blot - Anti-TRIM2 antibody (AB3942)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise