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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-TRIF (TICAM1) 抗体 [EPR24995-152] (ab302562)

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Western blot - Anti-TRIF (TICAM1) antibody [EPR24995-152] (ab302562)
  • Western blot - Anti-TRIF (TICAM1) antibody [EPR24995-152] (AB302562)
  • Western blot - Anti-TRIF (TICAM1) antibody [EPR24995-152] (AB302562)
  • Immunoprecipitation - Anti-TRIF (TICAM1) antibody [EPR24995-152] (AB302562)
  • Anti-TRIF (TICAM1) antibody [EPR24995-152] (AB302562)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24995-152] to TRIF
  • Suitable for: IP, WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-TRIF (TICAM1) antibody [EPR24995-152]
    TRIF 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR24995-152] to TRIF

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IP, WBmore details
    適用なし: Flow Cyt (Intra),ICC/IF or IHC-P

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Raji, Ramos, HL-60, HeLa and U-87 MG cell lysate. IP: Raji cell lysate.

  • 特記事項

    ab302562 does not react in: WB with mouse and rat samples; IHC-P with human, mouse, and rat specimens; ICC/IF and intracellular flow cytometry with human tissues. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR24995-152

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302562の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
1/30.
WB
1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 76 kDa).
特記事項
IP
1/30.
WB
1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 76 kDa).
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF or IHC-P.

ターゲット情報

  • 機能

    Involved in innate immunity against invading pathogens. Adapter used by TLR3 and TLR4 (through TICAM2) to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TRIF recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively.

  • 組織特異性

    Ubiquitously expressed but with higher levels in liver.

  • 配列類似性

    Contains 1 TIR domain.

  • ドメイン

    The N-terminal region is essential for activation of the IFNB promoter activity.

  • 翻訳後修飾

    Phosphorylated by TBK1.
  • Information by UniProt

  • 参照データベース

    • 別名

      • IIAE6 antibody
      • MGC35334 antibody
      • MyD88 3 antibody
      • Proline rich vinculin and TIR domain containing protein B antibody
      • Proline-rich antibody
      • PRVTIRB antibody
      • Putative NF kappa B activating protein 502H antibody
      • Putative NF-kappa-B-activating protein 502H antibody
      • Putative NFkB activating protein antibody
      • TCAM1_HUMAN antibody
      • TICAM 1 antibody
      • TICAM-1 antibody
      • Ticam1 antibody
      • TIR domain containing adapter molecule 1 antibody
      • TIR domain containing adapter protein inducing IFN beta antibody
      • TIR domain containing adaptor inducing interferon beta antibody
      • TIR domain-containing adapter molecule 1 antibody
      • TIR domain-containing adapter protein inducing IFN-beta antibody
      • Toll interleukin 1 receptor domain containing adapter protein inducing interferon beta antibody
      • Toll like receptor adaptor molecule 1 antibody
      • Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta antibody
      • TRIF antibody
      • TRIF protein antibody
      • vinculin and TIR domain-containing protein B antibody
      see all

    画像

    • Western blot - Anti-TRIF (TICAM1) antibody [EPR24995-152] (ab302562)
      Western blot - Anti-TRIF (TICAM1) antibody [EPR24995-152] (ab302562)
      All lanes : Anti-TRIF (TICAM1) antibody [EPR24995-152] (ab302562) at 1/1000 dilution

      Lane 1 : Wild-type U-87 MG cell lysate
      Lane 2 : TICAM1 knockout U-87 MG cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : HL-60 cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 76 kDa
      Observed band size: 85 kDa why is the actual band size different from the predicted?



      Western blot: Anti-TICAM1 antibody [EPR24995-152] (ab302562) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab302562 was shown to bind specifically to TICAM1. A band was observed at 85 kDa in wild-type U-87 MG cell lysates with no signal observed at this size in TICAM1 knockout cell line. To generate this image, wild-type and TICAM1 knockout U-87 MG cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    • Western blot - Anti-TRIF (TICAM1) antibody [EPR24995-152] (AB302562)
      Western blot - Anti-TRIF (TICAM1) antibody [EPR24995-152] (AB302562)
      All lanes : Anti-TRIF (TICAM1) antibody [EPR24995-152] (ab302562) at 1/1000 dilution

      Lane 1 : Raji (human Burkitt's lymphoma b lymphocyte) whole cell lysate
      Lane 2 : HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Performed under non-reducing conditions.

      Predicted band size: 76 kDa
      Observed band size: 98 kDa why is the actual band size different from the predicted?


      Exposure time: 59 seconds


      Diluting and blocking buffer and concentration: 5% NFDM/TBST. 

      Lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

      Negative control: HL-60.

    • Western blot - Anti-TRIF (TICAM1) antibody [EPR24995-152] (AB302562)
      Western blot - Anti-TRIF (TICAM1) antibody [EPR24995-152] (AB302562)
      All lanes : Anti-TRIF (TICAM1) antibody [EPR24995-152] (ab302562) at 1/1000 dilution

      Lane 1 : Ramos (human Burkitt's lymphoma b lymphocyte) whole cell lysate
      Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Performed under non-reducing conditions.

      Predicted band size: 76 kDa
      Observed band size: 98 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Diluting and blocking buffer and concentration: 5% NFDM/TBST. 

      Lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

      The identity of the lower MW band at approximately 18 kDa is unknown.

    • Immunoprecipitation - Anti-TRIF (TICAM1) antibody [EPR24995-152] (AB302562)
      Immunoprecipitation - Anti-TRIF (TICAM1) antibody [EPR24995-152] (AB302562)

      TRIF (TICAM1) was immunoprecipitated from 0.35 mg Raji (human Burkitt's lymphoma b lymphocyte) whole cell lysate with ab302562 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302562 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Raji (human burkitt's lymphoma b lymphocyte) whole cell lysate 10 µg (Input)

      Lane 2: ab302562 IP in Raji whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab302562 in Raji whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes

       Observed MW (kDa): 98. 

    • Anti-TRIF (TICAM1) antibody [EPR24995-152] (AB302562)
      Anti-TRIF (TICAM1) antibody [EPR24995-152] (AB302562)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab302562 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302562 は 1 報の論文で使用されています。

    • Yue L  et al. Toll-like receptor 4 promotes the inflammatory response in septic acute kidney injury by promoting p38 mitogen-activated protein kinase phosphorylation. J Bioenerg Biomembr 55:353-363 (2023). PubMed: 37605037

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