Anti-Trichohyalin 抗体 [AE15] (ab58755)
![Western blot - Anti-Trichohyalin antibody [AE15] (ab58755)](https://www.abcam.co.jp/ps/products/58/ab58755/Images/Trichohyalin-Primary-antibodies-ab58755-2.jpg "Western blot - Anti-Trichohyalin antibody [AE15] (ab58755)")
Key features and details
- Mouse monoclonal [AE15] to Trichohyalin
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Trichohyalin antibody [AE15]
Trichohyalin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [AE15] to Trichohyalin -
由来種
Mouse -
特異性
This antibody recognises a 220K trichohyalin granular protein, a marker for hair follicular differentiation. -
アプリケーション
適用あり: WB, IHC-P, IHC-Frmore details -
種交差性
交差種: Mouse, Human -
免疫原
Tissue, cells or virus corresponding to Human Trichohyalin.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
AE15 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab58755の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 220 kDa.
|
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| IHC-P | (1) |
Use at an assay dependent concentration. PubMed: 19038788
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| IHC-Fr | (1) |
Use at an assay dependent concentration. PubMed: 19786578
|
| 特記事項 |
|---|
|
WB
1/1000. Predicted molecular weight: 220 kDa. |
|
IHC-P
Use at an assay dependent concentration. PubMed: 19038788 |
|
IHC-Fr
Use at an assay dependent concentration. PubMed: 19786578 |
ターゲット情報
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機能
Intermediate filament-associated protein that associates in regular arrays with keratin intermediate filaments (KIF) of the inner root sheath cells of the hair follicle and the granular layer of the epidermis. It later becomes cross-linked to KIF by isodipeptide bonds. It may serve as scaffold protein, together with involucrin, in the organization of the cell envelope or even anchor the cell envelope to the KIF network. It may be involved in its own calcium-dependent postsynthetic processing during terminal differentiation. -
組織特異性
Found in the hard keratinizing tissues such as the inner root sheath (IRS) of hair follicles and medulla, and in the filiform papillae of dorsal tongue epithelium. -
配列類似性
Belongs to the S100-fused protein family.
Contains 2 EF-hand domains. -
発生段階
Expressed during late differentiation of the epidermis. -
ドメイン
Consists of nine domains. Domain 1 contains two EF-hand calcium-binding domains. Domains 2-4, 6, and 8 are almost entirely alpha-helical, configured as a series of peptide repeats of varying regularity, and are thought to form a single-stranded alpha-helical rod stabilized by ionic interactions. Domain 6 is the most regular and may bind KIF directly by ionic interactions. Domains 5 and 7 are less well organized and may induce folds in the molecule. Domain 9 contains the C-terminus, conserved among different species. -
翻訳後修飾
Substrate of transglutaminase. Some 200 arginines are probably converted to citrullines by peptidylarginine deimidase. - Information by UniProt
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参照データベース
- Entrez Gene: 7062 Human
- Entrez Gene: 99681 Mouse
- SwissProt: Q07283 Human
- Unigene: 432416 Human
- Unigene: 553300 Human
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別名
- 220K trichohyalin granular protein antibody
- Hair follicle protein AHF antibody
- MGC157889 antibody
see all
画像
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Western blot - Anti-Trichohyalin antibody [AE15] (ab58755)Anti-Trichohyalin antibody [AE15] (ab58755) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 220 kDa
Observed band size: 220 kDa
Additional bands at: 165 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab58755 は 17 報の論文で使用されています。
- Shi TW et al. Effects of NCSTN Mutation on Hair Follicle Components in Mice. Dermatology 239:60-71 (2023). PubMed: 35843211
- Makino T et al. Altered expression of S100 fused-type proteins in an atopic dermatitis skin model. Exp Dermatol 32:2160-2165 (2023). PubMed: 36995036
- Leng X et al. Dissociated skin cells regenerate hair follicles in a microwound, "The Punch Assay". Exp Dermatol N/A:N/A (2018). PubMed: 30033549
- Tripurani SK et al. Suppression of Wnt/ß-catenin signaling by EGF receptor is required for hair follicle development. Mol Biol Cell 29:2784-2799 (2018). PubMed: 30188763
- Sardella C et al. Delayed Hair Follicle Morphogenesis and Hair Follicle Dystrophy in a Lipoatrophy Mouse Model of Pparg Total Deletion. J Invest Dermatol 138:500-510 (2018). PubMed: 28964716