Anti-TRFP/MED20 抗体 (ab67800)
Key features and details
- Mouse polyclonal to TRFP/MED20
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-TRFP/MED20 antibody -
製品の詳細
Mouse polyclonal to TRFP/MED20 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human TRFP/MED20. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
-
ポジティブ･コントロール
- TRFP/MED20 transfected 293T cell lysate and Hela cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab67800の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).
|
|
| ICC/IF |
Use a concentration of 10 µg/ml.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa). |
|
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
-
関連性
TRFP / MED20 is a member of the mediator complex that can act as both a positive and negative regulator of transcription. The mediator complex interacts with RNA pol II and its associated proteins, the general transcription factors. Mediator proteins serve as a bridge connecting the activator and basal transcription machinery. -
細胞内局在
Nucleus -
参照データベース
- Entrez Gene: 9477 Human
- SwissProt: Q9H944 Human
-
別名
- MED20 antibody
- Mediator complex subunit 20 antibody
- TRF proximal protein homolog antibody
- TRFP antibody
画像
-
Western blot - Anti-TRFP/MED20 antibody (ab67800)All lanes : Anti-TRFP/MED20 antibody (ab67800) at 1/500 dilution
Lane 1 : TRFP/MED20 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 23 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-TRFP/MED20 antibody (ab67800)ab67800 at 10µg/ml staining TRFP/MED20 in Hela cells.
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (0)
ab67800 は論文での使用が確認できていません。
