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AB1423

Anti-TRF1 抗体

Anti-TRF1 antibody

2

(4 Reviews)

|

(40 Publications)

Rabbit Polyclonal TRF1 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 40 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TERF1.

別名を表示する

PIN2, TRBF1, TRF, TRF1, TERF1, Telomeric repeat-binding factor 1, NIMA-interacting protein 2, TTAGGG repeat-binding factor 1, Telomeric protein Pin2/TRF1

3 Images
Western blot - Anti-TRF1 antibody (AB1423)
  • WB

Supplier Data

Western blot - Anti-TRF1 antibody (AB1423)

Western blot labelling TRF1 in HL-60 (Human promyelocytic leukemia cell line) whole cell extract using ab1423 at 1/2000. A single band at 60-65 kD is detected.

All lanes:

Western blot - Anti-TRF1 antibody (ab1423) at 1/2000 dilution

All lanes:

HL-60 (Human promyelocytic leukemia cell line) whole cell extract

Predicted band size: 50 kDa

Observed band size: 60-65 kDa

false

Immunocytochemistry - Anti-TRF1 antibody (AB1423)
  • ICC

Supplier Data

Immunocytochemistry - Anti-TRF1 antibody (AB1423)

The cells in the first image are probably in a stage of the cell cycle where the signal is not so clear. In this image the antibody is shown to recognise the same signal but staining is shown as 20-30 spots (punctate foci).

Immunocytochemistry - Anti-TRF1 antibody (AB1423)
  • ICC

Unknown

Immunocytochemistry - Anti-TRF1 antibody (AB1423)

Cells transfected with a TRF1-GFP contruct. On the left : immunofluorescence with the anti-TRF1 antibody used at 1/300, with a Texas Red-conjugated secondary antibody. In the centre : fluorescence from the GFP. Right : merge of both images showing overlap of GFP fluorescence and antibody staining.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

ICC, WB

applications

免疫原

Recombinant Full Length Protein corresponding to Human TERF1.

P54274

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>An additional non specific band at 35 kDa may be observed. Denatured whole cell extracts (prepare total cell extracts with 6M urea, 1% SDS, 150 mM NaCl, 25mM Tris ph8, and then sonicate for 10 seconds)and TBS based buffers with 0.5% Tween 20 are recommended.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

出荷温度及び保存条件

製品の状態
Liquid
精製度
Whole antiserum
バッファー組成
pH: 7.2 Preservative: 0.05% Sodium azide
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TRF1 also known as TERF1 (Telomeric Repeat Binding Factor 1) is an important protein involved in telomere maintenance. This protein has a molecular mass of approximately 55 kDa. TRF1 localizes specifically to telomeres and plays an essential role in shelterin complex formation a specialized protein complex that protects chromosome ends. TRF1 is highly expressed in proliferating cells and tissues especially those with a high rate of cell division.
Biological function summary

TRF1 contributes to the stabilization of telomere structure and regulates telomere length. TRF1 is a part of the shelterin complex which also includes proteins such as TRF2 POT1 TIN2 RAP1 and TPP1. The shelterin complex prevents the telomeres from being recognized as DNA damage and regulates their replicative processes. TRF1 interacts directly with telomeric DNA influencing replication and modulating access to telomerase the enzyme responsible for telomere elongation.

Pathways

TRF1 plays a significant role in telomere maintenance and the cell cycle. The protein is critical in the DNA damage response pathway where it prevents unwanted DNA repair activities at telomeres. In this context TRF1 interacts with proteins like ATM and ATR which are important for signaling and responding to DNA damage. TRF1's interaction with these pathways helps maintain genomic stability and cellular homeostasis.

Alterations in TRF1 function can contribute to cancer and aging-related diseases. High levels or overexpression of TRF1 are frequently observed in certain cancers where it may help tumor cells maintain telomeres and proliferate indefinitely. Conversely lower TRF1 levels are associated with premature aging disorders such as dyskeratosis congenita. In these conditions the connection between TRF1 and TRF2 within the shelterin complex plays an important role in maintaining telomere integrity affecting disease development and progression.

製品プロトコール

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ターゲットの情報

Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
See full target information TERF1

文献 (40)

Recent publications for all applications. Explore the full list and refine your search

Methods in molecular biology (Clifton, N.J.) 2444:141-159 PubMed35290636

2022

Targeted Formation of 8-Oxoguanine in Telomeres.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan P Barnes,Sanjana A Thosar,Elise Fouquerel,Patricia L Opresko

Nature communications 13:760 PubMed35140211

2022

Prime editing efficiency and fidelity are enhanced in the absence of mismatch repair.

Applications

Unspecified application

Species

Unspecified reactive species

J Ferreira da Silva,G P Oliveira,E A Arasa-Verge,C Kagiou,A Moretton,G Timelthaler,J Jiricny,J I Loizou

IET nanobiotechnology 16:14-24 PubMed34862858

2021

Bone marrow mesenchymal stem cell-derived exosomes improve renal fibrosis by reducing the polarisation of M1 and M2 macrophages through the activation of EP2 receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqing Lu,Lulu Yang,Xiao Chen,Jing Liu,Anqi Nie,Xiaolan Chen

Metallomics : integrated biometal science 13: PubMed34021581

2021

Pt-ttpy, a G-quadruplex binding platinum complex, induces telomere dysfunction and G-rich regions DNA damage.

Applications

ChIP

Species

Human

Samar Ali,Emilia Puig Lombardi,Deepanjan Ghosh,Tao Jia,Géraldine Vitry,Lina Saker,Joël Poupon,Marie-Paule Teulade-Fichou,Alain Nicolas,Arturo Londono-Vallejo,Sophie Bombard

RNA biology 18:2261-2277 PubMed33749516

2021

Transcriptional regulation of telomeric repeat-containing RNA by acridine derivatives.

Applications

WB

Species

Human

Shuangshuang Kang,Jiaojiao Cao,Meiling Zhang,Xiaoya Li,Qian-Liang Guo,Huang Zeng,Zuzhuang Wei,Xue Gong,Jing Wang,Bobo Liu,Bing Shu,Xiaoli Xu,Zhi-Shu Huang,Ding Li

Frontiers in cell and developmental biology 9:617225 PubMed33553179

2021

Telomere Dysfunction in Oocytes and Embryos From Obese Mice.

Applications

IF

Species

Mouse

Juan Ge,Congyang Li,Hongzheng Sun,Yongan Xin,Shuai Zhu,Yuan Liu,Shoubin Tang,Longsen Han,Zhenyue Huang,Qiang Wang

International journal of molecular sciences 21: PubMed31877678

2019

A Novel Tissue and Stem Cell Specific TERF1 Splice Variant Is Downregulated in Tumour Cells.

Applications

IF, IHC, IHC, WB, WB, IF

Species

Human, Human, Mouse, Human, Mouse, Mouse

Yousef Ashraf Tawfik Morcos,Gregoire Najjar,Sabine Meessen,Britta Witt,Anca Azoitei,Mukesh Kumar,Gamal Wakileh,Klaus Schwarz,Hubert Schrezenmeier,Friedemann Zengerling,Christian Bolenz,Cagatay Günes

Aging 11:1965-1976 PubMed30926765

2019

SIRT6 participates in the quality control of aged oocytes via modulating telomere function.

Applications

ICC/IF

Species

Mouse

Juan Ge,Congyang Li,Chunling Li,Zhenyue Huang,Juan Zeng,Longsen Han,Qiang Wang

Nature communications 9:3583 PubMed30181605

2018

Local enrichment of HP1alpha at telomeres alters their structure and regulation of telomere protection.

Applications

WB

Species

Human

Tracy T Chow,Xiaoyu Shi,Jen-Hsuan Wei,Juan Guan,Guido Stadler,Bo Huang,Elizabeth H Blackburn

Molecular and cellular biology 38: PubMed29581185

2018

The C-Terminal Extension Unique to the Long Isoform of the Shelterin Component TIN2 Enhances Its Interaction with TRF2 in a Phosphorylation- and Dyskeratosis Congenita Cluster-Dependent Fashion.

Applications

ChIP

Species

Human

Nya D Nelson,Lois M Dodson,Laura Escudero,Ann T Sukumar,Christopher L Williams,Ivana Mihalek,Alessandro Baldan,Duncan M Baird,Alison A Bertuch
View all publications

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