AB192629

Anti-TRF1 抗体 [572C]

Name: Anti-TRF1 抗体 [572C] (ab192629) | Abcam
Brand: Abcam Limited
SKU: AB192629
Price: 58000 JPY
Availability: InStock
Rating: 3 (2 reviews)

Anti-TRF1 antibody [572C]

(2 Reviews)

(4 Publications)

Anti-TRF1 antibody [572C] (ab192629) is a rat monoclonal antibody detecting TRF1 in Western Blot, ICC/IF. Suitable for Human, Mouse.

別名を表示する

PIN2, TRBF1, TRF, TRF1, TERF1, Telomeric repeat-binding factor 1, NIMA-interacting protein 2, TTAGGG repeat-binding factor 1, Telomeric protein Pin2/TRF1

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-TRF1 antibody [572C] (AB192629)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TRF1 antibody [572C] (AB192629)

Immunocytochemistry/ Immunofluorescent analysis of mouse blastocyte labeling TRF1 with ab192629 at a 1/100 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TRF1 antibody [572C] (AB192629)

Immunocytochemistry/ Immunofluorescent analysis of frozen cytospins preparations of mTRF1 expressed in HEK293T labeling TRF1 using ab192629. Negative control on right used mTRF2-GFP expressed in HEK293T.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TRF1 antibody [572C] (AB192629)

Immunocytochemistry/ Immunofluorescent analysis of HEK-TRF1, HEK-GFP-TRF2, IPS-GFP-TRF1homo and IPS-shTRF1homo cell lines labeling TRF1 with undiluted ab192629.

Supplier Data

Western blot - Anti-TRF1 antibody [572C] (AB192629)

All lanes:

Western blot - Anti-TRF1 antibody [572C] (ab192629) at 1/500 dilution

Lane 1:

Mefs TRF1 +/+ nuclear extract at 30 µg

Lane 2:

Mefs TRF1 KO nuclear extract at 30 µg

Predicted band size: 50 kDa

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Supplier Data

Western blot - Anti-TRF1 antibody [572C] (AB192629)

ab192629 used undiluted for all lanes.

All lanes:

Western blot - Anti-TRF1 antibody [572C] (ab192629)

Lane 1:

IPS-GFP-TRF1 homo at 100 µg

Lane 2:

IPS-GFP-TRF1 heter at 100 µg

Lane 3:

IPS-sh TRF1 at 100 µg

Lane 4:

IPS-sh TRF1 puro at 100 µg

Lane 5:

HEK-TRF1 at 20 µg

Lane 6:

HEK-FGFR2 at 20 µg

Lane 7:

NIH-3T3 at 100 µg

Predicted band size: 50 kDa

false

Key facts

宿主種

Rat

クローン性

Monoclonal

クローン番号

572C

アイソタイプ

IgG2a

キャリアフリー

交差種

Mouse, Human

アプリケーション

WB, ICC/IF

applications

免疫原

Recombinant Full Length Protein corresponding to Mouse Terf1.

P70371

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-TRF1 antibody [572C] (ab192629) is a rat monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

What is the molecular weight of TRF1?
Anti-TRF1 [572C] (ab192629) specifically detects a band for TRF1 (UniProt: P54274) at a molecular weight of 50kDa.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

精製に関する特記事項

HiTrap protein G columns

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Do Not Freeze

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TRF1 also known as TERF1 (Telomeric Repeat Binding Factor 1) is an important protein involved in telomere maintenance. This protein has a molecular mass of approximately 55 kDa. TRF1 localizes specifically to telomeres and plays an essential role in shelterin complex formation a specialized protein complex that protects chromosome ends. TRF1 is highly expressed in proliferating cells and tissues especially those with a high rate of cell division.

Biological function summary

TRF1 contributes to the stabilization of telomere structure and regulates telomere length. TRF1 is a part of the shelterin complex which also includes proteins such as TRF2 POT1 TIN2 RAP1 and TPP1. The shelterin complex prevents the telomeres from being recognized as DNA damage and regulates their replicative processes. TRF1 interacts directly with telomeric DNA influencing replication and modulating access to telomerase the enzyme responsible for telomere elongation.

Pathways

TRF1 plays a significant role in telomere maintenance and the cell cycle. The protein is critical in the DNA damage response pathway where it prevents unwanted DNA repair activities at telomeres. In this context TRF1 interacts with proteins like ATM and ATR which are important for signaling and responding to DNA damage. TRF1's interaction with these pathways helps maintain genomic stability and cellular homeostasis.

Alterations in TRF1 function can contribute to cancer and aging-related diseases. High levels or overexpression of TRF1 are frequently observed in certain cancers where it may help tumor cells maintain telomeres and proliferate indefinitely. Conversely lower TRF1 levels are associated with premature aging disorders such as dyskeratosis congenita. In these conditions the connection between TRF1 and TRF2 within the shelterin complex plays an important role in maintaining telomere integrity affecting disease development and progression.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length (PubMed : 31595153). Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.

See full target information TERF1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0332202 PubMed40991530

2025

Investigating the anti-osteosarcoma effects of Patchouli alcohol through protein network mapping and in vitro experiments.

Applications

Unspecified application

Species

Unspecified reactive species

Zeyu Zhan,He Pang,Hang Wu,Lijun Song,Bo Wei

World journal of gastrointestinal oncology 17:104522 PubMed40487942

2025

Simvastatin inhibits proliferation and migration, promotes oxidative stress and ferroptosis in colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Hao Ge,Zhi-Min Fan,Ting Lu,Lei He,Meng Li,Hao-Ran Zhao,Qiang Leng

Frontiers in cell and developmental biology 10:862506 PubMed35493072

2022

MFN2 Deficiency Impairs Mitochondrial Functions and PPAR Pathway During Spermatogenesis and Meiosis in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tianren Wang,Yuan Xiao,Zhe Hu,Jingkai Gu,Renwu Hua,Zhuo Hai,Xueli Chen,Jian V Zhang,Zhiying Yu,Ting Wu,William S B Yeung,Kui Liu,Chenxi Guo

Frontiers in cell and developmental biology 9:749116 PubMed34604243

2021

TRF1 Depletion Reveals Mutual Regulation Between Telomeres, Kinetochores, and Inner Centromeres in Mouse Oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Hyuk-Joon Jeon,Jeong Su Oh

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRF1 抗体 [572C]

Anti-TRF1 antibody [572C]

Immunocytochemistry/ Immunofluorescence - Anti-TRF1 antibody [572C] (AB192629)

Immunocytochemistry/ Immunofluorescence - Anti-TRF1 antibody [572C] (AB192629)

Immunocytochemistry/ Immunofluorescence - Anti-TRF1 antibody [572C] (AB192629)

Western blot - Anti-TRF1 antibody [572C] (AB192629)

Western blot - Anti-TRF1 antibody [572C] (AB192629)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise