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リコンビナントRabMAb

Anti-TREX1 抗体 [EPR25101-12] (BSA and Azide free) (ab300446)

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Western blot - Anti-TREX1 antibody [EPR25101-12] (BSA and Azide free) (AB300446)
  • Western blot - Anti-TREX1 antibody [EPR25101-12] (BSA and Azide free) (AB300446)
  • Western blot - Anti-TREX1 antibody [EPR25101-12] (BSA and Azide free) (AB300446)
  • Immunocytochemistry/ Immunofluorescence - Anti-TREX1 antibody [EPR25101-12] (BSA and Azide free) (ab300446)
  • Flow Cytometry - Anti-TREX1 antibody [EPR25101-12] (BSA and Azide free) (AB300446)
  • Immunoprecipitation - Anti-TREX1 antibody [EPR25101-12] (BSA and Azide free) (AB300446)
  • Anti-TREX1 antibody [EPR25101-12] (BSA and Azide free) (AB300446)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25101-12] to TREX1 - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IP, ICC/IF
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-TREX1 antibody [EPR25101-12] (BSA and Azide free)
    TREX1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25101-12] to TREX1 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt, WB, IP, ICC/IFmore details
    適用なし: IHC-P

  • 種交差性

    交差種: Mouse
    非交差種: Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: J774A.1 and RAW 264.7 cells treated with LPS whole cell lysate. Mouse spleen and thymus tissue lysate. ICC/IF: RAW 264.7 cells treated with LPS. Flow Cyt: RAW 264.7 cells treated with LPS. IP: RAW 264.7 cells treated with LPS whole cell lysate.

  • 特記事項

    ab300446 is a carrier free version of ab300445

     

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300446の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 39 kDa.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
特記事項
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 39 kDa.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
追加情報
Is unsuitable for IHC-P.

ターゲット情報

  • 関連性

    TREX1 is the major 3'->5' DNA exonuclease in human cells. The protein is a non processive exonuclease that may serve a proofreading function for a human DNA polymerase. It is also a component of the SET complex, and acts to rapidly degrade 3' ends of nicked DNA during granzyme A mediated cell death. Mutations in this gene result in Aicardi Goutieres syndrome, chilblain lupus, and Cree encephalitis. Multiple transcript variants encoding different isoforms have been found for this gene.

  • 細胞内局在

    Cytoplasmic, Endoplasmic reticulum and Nuclear

  • 参照データベース

    • 別名

      • 3' 5' exonuclease TREX1 antibody
      • 3' repair exonuclease 1 antibody
      • AGS1 antibody
      • AGS5 antibody
      • CRV antibody
      • Deoxyribonuclease III, dnaQ/mutD (E. coli) like antibody
      • DKFZp434J0310 antibody
      • DNase III antibody
      • DRN3 antibody
      • HERNS antibody
      • Three prime repair exonuclease 1 antibody
      • TREX1 antibody
      see all

    画像

    • Western blot - Anti-TREX1 antibody [EPR25101-12] (BSA and Azide free) (AB300446)
      Western blot - Anti-TREX1 antibody [EPR25101-12] (BSA and Azide free) (AB300446)
      All lanes : Anti-TREX1 antibody [EPR25101-12] (ab300445) at 1/1000 dilution

      Lane 1 : Untreated J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate
      Lane 2 : J774A.1 treated with 1ug/ml LPS for 18h whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 39 kDa
      Observed band size: 30,33 kDa why is the actual band size different from the predicted?


      Exposure time: 37 seconds


      This data was developed using ab300445, the same antibody clone in a different buffer formulation.

      Blocking / Diluting buffer: 5% NFDM/TBST.

      The expression of TREX1 is upregulated in response to LPS treatment (PMID: 24218451).

      The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 11279105; PMID: 26320659).

    • Western blot - Anti-TREX1 antibody [EPR25101-12] (BSA and Azide free) (AB300446)
      Western blot - Anti-TREX1 antibody [EPR25101-12] (BSA and Azide free) (AB300446)
      All lanes : Anti-TREX1 antibody [EPR25101-12] (ab300445) at 1/1000 dilution

      Lane 1 : Untreated RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
      Lane 2 : RAW 264.7 treated with 500ng/ml LPS for 24h whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 39 kDa
      Observed band size: 30, 33 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      This data was developed using ab300445, the same antibody clone in a different buffer formulation.

      Blocking / Diluting buffer: 5% NFDM/TBST.

      The expression of TREX1 is upregulated in response to LPS treatment (PMID: 24218451).

      The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 11279105; PMID: 26320659).

    • Western blot - Anti-TREX1 antibody [EPR25101-12] (BSA and Azide free) (AB300446)
      Western blot - Anti-TREX1 antibody [EPR25101-12] (BSA and Azide free) (AB300446)
      All lanes : Anti-TREX1 antibody [EPR25101-12] (ab300445) at 1/1000 dilution

      Lane 1 : Mouse spleen tissue lysate
      Lane 2 : Mouse thymus tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 39 kDa
      Observed band size: 30, 33 kDa why is the actual band size different from the predicted?


      Exposure time: 59 seconds


      This data was developed using ab300445, the same antibody clone in a different buffer formulation.

      Blocking / Diluting buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-TREX1 antibody [EPR25101-12] (BSA and Azide free) (ab300446)
      Immunocytochemistry/ Immunofluorescence - Anti-TREX1 antibody [EPR25101-12] (BSA and Azide free) (ab300446)

      This data was developed using ab300445, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophages) labelling TREX1 with ab300445 at 1/50 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/2000 dilution (green). Confocal image showing increased cytoplasmic and nuclear staining in RAW 264.7 cells treated with lipopolysaccharides (LPS, 500 ng/mL) for 24 h. ab195889. Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used as a counterstain (red). The nuclear counterstain is DAPI (blue).

    • Flow Cytometry - Anti-TREX1 antibody [EPR25101-12] (BSA and Azide free) (AB300446)
      Flow Cytometry - Anti-TREX1 antibody [EPR25101-12] (BSA and Azide free) (AB300446)

      This data was developed using ab300445, the same antibody clone in a different buffer formulation.

      Flow Cytometry analysis of RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 500 ng/ml LPS for 24 hours (Red) / Untreated control (Green) labeling TREX1 with ab300445 at a 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at a 1/2000 dilution. Rabbit monoclonal isotype control (ab172730) - Alexa Fluor® 488 (Black) Unlabelled control - Cells without incubation with primary or secondary antibodies (Blue).

    • Immunoprecipitation - Anti-TREX1 antibody [EPR25101-12] (BSA and Azide free) (AB300446)
      Immunoprecipitation - Anti-TREX1 antibody [EPR25101-12] (BSA and Azide free) (AB300446)

      This data was developed using ab300445, the same antibody clone in a different buffer formulation.

      TREX1 was immunoprecipitated from 10 μg RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 500ng/ml LPS for 24h whole cell lysate with ab300445 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab300445 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

      Lane 1: RAW 264.7 treated with 500ng/ml LPS for 24h whole cell lysate 10 μg (Input).

      Lane 2: RAW 264.7 treated with 500ng/ml LPS for 24h whole cell lysate.   

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300445 in RAW 264.7 treated with 500ng/ml LPS for 24h whole cell lysate.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 5.5 seconds.

      Observed MW(KDa): 30, 33.

    • Anti-TREX1 antibody [EPR25101-12] (BSA and Azide free) (AB300446)
      Anti-TREX1 antibody [EPR25101-12] (BSA and Azide free) (AB300446)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300446 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300446 は論文での使用が確認できていません。

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