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リコンビナントRabMAb

Anti-TREM2 抗体 [EPR23177-106] (ab245227)

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Flow Cytometry - Anti-TREM2 antibody [EPR23177-106] (ab245227)
  • Immunocytochemistry/ Immunofluorescence - Anti-TREM2 antibody [EPR23177-106] (ab245227)
  • Anti-TREM2 antibody [EPR23177-106] (ab245227)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23177-106] to TREM2
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 647 APC Carrier Free PE

製品の概要

  • 製品名

    Anti-TREM2 antibody [EPR23177-106]
    TREM2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23177-106] to TREM2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt, ICC/IFmore details
    適用なし: IHC-P,IP or WB

  • 種交差性

    交差種: Mouse

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • ICC/IF: RAW 264.7 cells. Flow Cyt: RAW 267.4 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR23177-106

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab245227の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
1/60.
ICC/IF
1/100.
特記事項
Flow Cyt
1/60.
ICC/IF
1/100.
追加情報
Is unsuitable for IHC-P,IP or WB.

ターゲット情報

  • 機能

    May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells.

  • 組織特異性

    Expressed on macrophages and dendritic cells but not on granulocytes or monocytes. In the CNS strongest expression seen in the basal ganglia, corpus callosum, medulla oblongata and spinal cord.

  • 関連疾患

    Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns.

  • 配列類似性

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • 細胞内局在

    Secreted and Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • TREM 2 antibody
      • TREM-2 antibody
      • TREM2 antibody
      • TREM2_HUMAN antibody
      • TREM2a antibody
      • TREM2b antibody
      • TREM2c antibody
      • Trggering receptor expressed on myeloid cells 2 antibody
      • Trggering receptor expressed on myeloid cells 2a antibody
      • Triggering receptor expressed on monocytes 2 antibody
      • Triggering receptor expressed on myeloid cells 2 antibody
      see all

    画像

    • Flow Cytometry - Anti-TREM2 antibody [EPR23177-106] (ab245227)
      Flow Cytometry - Anti-TREM2 antibody [EPR23177-106] (ab245227)

      Flow cytometric analysis of Neuro-2a (Mouse neuroblastoma neuroblast, Left) / RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells labelling Trem2 with ab245227 at 1/60 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.

      Negative control: Neuro-2a (PMID: 19302484).

      Gated on viable cells.

      Brighter secondary antibody like Alexa Fluor® 647 is required to get desirable staining.

    • Immunocytochemistry/ Immunofluorescence - Anti-TREM2 antibody [EPR23177-106] (ab245227)
      Immunocytochemistry/ Immunofluorescence - Anti-TREM2 antibody [EPR23177-106] (ab245227)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Trem2 with ab245227 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and staining in RAW 264.7 cell line. Negative control: Neuro-2a (PMID: 19302484). ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    • Anti-TREM2 antibody [EPR23177-106] (ab245227)
      Anti-TREM2 antibody [EPR23177-106] (ab245227)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab245227 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab245227 は論文での使用が確認できていません。

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