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リコンビナントRabMAb

Anti-Trefoil Factor 3 抗体 [EPR26048-14] (ab300427)

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Western blot - Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)
  • Western blot - Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)
  • Western blot - Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)
  • Immunohistochemistry (Frozen sections) - Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)
  • Immunohistochemistry (Frozen sections) - Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)
  • Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26048-14] to Trefoil Factor 3
  • Suitable for: IHC-Fr, WB, IHC-P
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Trefoil Factor 3 antibody [EPR26048-14]
    Trefoil Factor 3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26048-14] to Trefoil Factor 3

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-Fr, WB, IHC-Pmore details
    適用なし: Flow Cyt or ICC/IF

  • 種交差性

    交差種: Mouse, Rat
    非交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse small intestine tissue lysate, Mouse colon tissue lysate, Rat colon tissue lysate. IHC-Fr: Mouse colon (fresh) and Rat colon (fresh) frozen tissue. IHC-P: Mouse colon and Rat colon FFPE tissue sections.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR26048-14

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300427の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
1/500.
WB
1/1000.
IHC-P
1/5000.
特記事項
IHC-Fr
1/500.
WB
1/1000.
IHC-P
1/5000.
追加情報
Is unsuitable for Flow Cyt or ICC/IF.

ターゲット情報

  • 機能

    Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).

  • 組織特異性

    Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus.

  • 配列類似性

    Contains 1 P-type (trefoil) domain.

  • 細胞内局在

    Secreted > extracellular space > extracellular matrix > apical lamina.
  • Information by UniProt

  • 参照データベース

    • 別名

      • hITF antibody
      • hP1.B antibody
      • Intestinal trefoil factor antibody
      • ITF antibody
      • mITF antibody
      • OTTMUSP00000021729 antibody
      • P1B antibody
      • Polypeptide P1.B antibody
      • TFF3 antibody
      • TFF3_HUMAN antibody
      • TFI antibody
      • TREFOIL antibody
      • Trefoil factor (intestinal) antibody
      • Trefoil factor 3 antibody
      see all

    画像

    • Western blot - Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)
      Western blot - Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)
      All lanes : Anti-Trefoil Factor 3 antibody [EPR26048-14] (ab300427) at 1/1000 dilution

      Lane 1 : Mouse small intestine tissue lysate
      Lane 2 : Mouse liver tissue lysate

      Lysates/proteins at 50 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Observed band size: 9 kDa why is the actual band size different from the predicted?


      Exposure time: 37 seconds


      Blocking and Diluting buffer and concentration: 5% NFDM/TBST

      Negative control: liver (PMID:7741746)
      The identity of the band at approximately 37 kDa (in lane 2) is unknown.

    • Western blot - Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)
      Western blot - Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)
      Anti-Trefoil Factor 3 antibody [EPR26048-14] (ab300427) at 1/1000 dilution + Mouse colon tissue lysate at 50 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Observed band size: 9 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST

    • Western blot - Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)
      Western blot - Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)
      All lanes : Anti-Trefoil Factor 3 antibody [EPR26048-14] (ab300427) at 1/1000 dilution

      Lane 1 : Rat colon tissue lysate
      Lane 2 : Rat liver tissue lysate

      Lysates/proteins at 50 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Observed band size: 9 kDa why is the actual band size different from the predicted?


      Exposure time: 36 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Negative control: liver (PMID: 7741746)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)

      Immunohistochemical analysis of paraffin-embedded mouse colon labeling Trefoil Factor 3 with ab300427 at 1/5000 dilution (0.108 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on goblet cells of mouse colon. The section was incubated with ab300427 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.     

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)

      Immunohistochemical analysis of paraffin-embedded mouse liver labeling Trefoil Factor 3 with ab300427 at 1/5000 dilution (0.108 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Negative control: No staining on mouse liver. The section was incubated with ab300427 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)

      Immunohistochemical analysis of paraffin-embedded rat colon labeling Trefoil Factor 3 with ab300427 at 1/5000 dilution (0.108 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on goblet cells of rat colon. The section was incubated with ab300427 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Frozen sections) - Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)
      Immunohistochemistry (Frozen sections) - Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned mouse colon (fresh) tissue labeling Trefoil Factor 3 with ab300427 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) at 1/1000 dilution (green). Positive staining on goblet cells of mouse colon. The nuclear counter stain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) at 1/1000 dilution.

      No antigen retrieval was carried out.

    • Immunohistochemistry (Frozen sections) - Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)
      Immunohistochemistry (Frozen sections) - Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned rat colon (fresh) tissue labeling Trefoil Factor 3 with ab300427 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) at 1/1000 dilution (green). Positive staining on goblet cells of rat colon. The nuclear counter stain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) at 1/1000 dilution.

      No antigen retrieval was carried out.

    • Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)
      Anti-Trefoil Factor 3 antibody [EPR26048-14] (AB300427)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300427 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300427 は論文での使用が確認できていません。

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