Anti-TRAP220/MED1 抗体 (ab245546)
Key features and details
- Rabbit polyclonal to TRAP220/MED1
- Suitable for: IP, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-TRAP220/MED1 antibody
TRAP220/MED1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TRAP220/MED1 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Orangutan
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免疫原
Synthetic peptide within Human TRAP220/MED1 aa 1525-1581. The exact sequence is proprietary. NP_004765.2
Database link: Q15648 -
ポジティブ･コントロール
- WB: RKO, HeLa, HEK-293T, K-562, Jurkat, NIH/3T3, CT26 and CH27 whole cell lysates. IP: HeLa whole cell lysate. IHC-P: Human lung carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab245546 was affinity purified using an epitope specific to TRAP220/MED1 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245546の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 168 kDa.
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| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 168 kDa. |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. -
組織特異性
Ubiquitously expressed. -
配列類似性
Belongs to the Mediator complex subunit 1 family. -
翻訳後修飾
Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3. - Information by UniProt
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参照データベース
- Entrez Gene: 5469 Human
- Entrez Gene: 19014 Mouse
- Omim: 604311 Human
- SwissProt: Q15648 Human
- SwissProt: Q925J9 Mouse
- Unigene: 643754 Human
- Unigene: 12926 Mouse
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別名
- Activator-recruited cofactor 205 kDa component antibody
- ARC205 antibody
- CRSP1 antibody
see all
画像
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Western blot - Anti-TRAP220/MED1 antibody (ab245546)All lanes : Anti-TRAP220/MED1 antibody (ab245546) at 0.04 µg/ml
Lane 1 : RKO (Human colon carinoma cell line) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 4 : K-562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate
Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 168 kDa
Exposure time: 3 seconds -
Western blot - Anti-TRAP220/MED1 antibody (ab245546)All lanes : Anti-TRAP220/MED1 antibody (ab245546) at 0.1 µg/ml
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 2 : CT26 (Mouse colon carcinoma fibroblast cell line) whole cell lysate
Lane 3 : CH27 (Mouse lymphoma cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 168 kDa
Exposure time: 3 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 antibody (ab245546)Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for TRAP220/MED1 using ab245546 at 1/1000 dilution in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab245546 は論文での使用が確認できていません。