Anti-TRAP1 抗体 [EPR5381] (ab109323)
![Western blot - Anti-TRAP1 antibody [EPR5381] (ab109323)](https://www.abcam.co.jp/ps/products/109/ab109323/Images/ab109323-122103-anti-trap1-antibody-epr5381-western-blot.jpg "Western blot - Anti-TRAP1 antibody [EPR5381] (ab109323)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5381] to TRAP1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-TRAP1 antibody [EPR5381]
TRAP1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5381] to TRAP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: K562, HL60, 293T and HeLa cell lysates IHC-P: Human kidney and liver tissues
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5381 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109323の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 80 kDa).
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 80 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Chaperone that expresses an ATPase activity. -
組織特異性
Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta. -
配列類似性
Belongs to the heat shock protein 90 family. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 10131 Human
- Omim: 606219 Human
- SwissProt: Q12931 Human
- Unigene: 30345 Human
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別名
- Heat shock protein 75 kDa antibody
- Heat shock protein 75 kDa, mitochondrial antibody
- HSP 75 antibody
see all
画像
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Western blot - Anti-TRAP1 antibody [EPR5381] (ab109323)All lanes : Anti-TRAP1 antibody [EPR5381] (ab109323) at 1/10000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HL60 cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 80 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP1 antibody [EPR5381] (ab109323)](https://www.abcam.co.jp/ps/products/109/ab109323/Images/ab109323-122102-anti-trap1-antibody-epr5381-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP1 antibody [EPR5381] (ab109323)ab109323, at a 1/100 dilution, staining TRAP1 in paraffin embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP1 antibody [EPR5381] (ab109323)](https://www.abcam.co.jp/ps/products/109/ab109323/Images/ab109323-122101-anti-trap1-antibody-epr5381-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP1 antibody [EPR5381] (ab109323)ab109323, at a 1/100 dilution, staining TRAP1 in paraffin embedded Human liver tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-TRAP1 antibody [EPR5381] (ab109323)](https://www.abcam.co.jp/ps/products/109/ab109323/Images/ab109323-4-benefits-of-recombinant-antibodies.png)
Anti-TRAP1 antibody [EPR5381] (ab109323)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab109323 は 4 報の論文で使用されています。
- Key J et al. Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling. Int J Mol Sci 24:N/A (2023). PubMed: 38139332
- Park HK et al. Interplay between TRAP1 and Sirtuin-3 Modulates Mitochondrial Respiration and Oxidative Stress to Maintain Stemness of Glioma Stem Cells. Cancer Res 79:1369-1382 (2019). PubMed: 30683653
- Gao C et al. Overexpression of the mitochondrial chaperone tumor necrosis factor receptor-associated protein 1 is associated with the poor prognosis of patients with colorectal cancer. Oncol Lett 15:5451-5458 (2018). PubMed: 29552185
- Megger DA et al. Proteomic differences between hepatocellular carcinoma and nontumorous liver tissue investigated by a combined gel-based and label-free quantitative proteomics study. Mol Cell Proteomics 12:2006-20 (2013). PubMed: 23462207