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AB228995

Anti-Transferrin Receptor 抗体 [EPR4012] - Low endotoxin, Azide free

Anti-Transferrin Receptor antibody [EPR4012] - Low endotoxin, Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Transferrin Receptor antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

別名を表示する

CD71, Transferrin receptor protein 1, TR, TfR, TfR1, Trfr, T9, p90, TFRC

1 Images
Flow Cytometry (Intracellular) - Anti-Transferrin Receptor antibody [EPR4012] - Low endotoxin, Azide free (AB228995)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Transferrin Receptor antibody [EPR4012] - Low endotoxin, Azide free (AB228995)

This Intracellular Intracellular Flow Cyt data was generated using the same anti-Transferrin Receptor antibody clone, EPR4012, in a different buffer formulation (cat

ab108985).

Overlay histogram showing Jurkat cells stained with ab108985 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108985, 1/100) for 30 min at 22�C. The secondary antibody used was DyLight� 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22�C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1�g/1x106cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

関連する標識済み抗体及び組成の異なる製品 (6)

  • Unconjugated

    Anti-Transferrin Receptor antibody [EPR4012]

  • Carrier free

    Anti-Transferrin Receptor antibody [EPR4012] - BSA and Azide free

  • 660 APC

    APC Anti-Transferrin Receptor antibody [EPR4012]

  • 578 PE

    PE Anti-Transferrin Receptor antibody [EPR4012]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Transferrin Receptor antibody [EPR4012]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Transferrin Receptor antibody [EPR4012]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR4012

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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製品の詳細

ab228995 is the carrier-free version of ab108985.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The transferrin receptor commonly referred to as TfR or CD71 is an integral membrane protein that facilitates the uptake of transferrin-bound iron into cells. This receptor has a molecular weight of around 95 kDa and often exists as a homodimer on the cell surface. It is widely expressed in many tissues especially in erythroid precursors and rapidly dividing cells. Alternate names for this receptor include TfR1 and TfR2 though they have distinct roles and distributions. Other transmembrane proteins like OX26 and MEM have been studied in relation to the transferrin receptor due to their involvement in drug delivery.
Biological function summary

TfR plays a critical role in iron homeostasis by mediating the internalization of transferrin and release of iron in the endosomes. It operates as part of the transferrin-transferrin receptor complex facilitating iron assimilation necessary for DNA synthesis and cell growth. Iron release involves acidifying endosomes allowing transferrin to bind with specific cellular receptors including alternate forms like beta 2 transferrin. The process subsequently contributes to erythropoiesis and various metabolic processes by regulating essential cellular iron levels.

Pathways

The transferrin receptor is central to iron metabolism and the receptor-mediated endocytosis pathway. It tightly interacts with transferrin and intracellular pathways process the iron released from transferrin within endosomes. The receptor's role in this pathway involves a dynamic with other proteins such as HFE and hepcidin. These interactions help control systemic iron levels linking closely to the maintenance of erythroid cell health and proliferation.

Disruptions in transferrin receptor function correlate with anemia and neurodegenerative disorders. In anemia related to iron deficiency impaired TfR activity reduces iron uptake culminating in insufficient erythropoiesis. Altered receptor expression or function also connects to neurological diseases like Alzheimer's where iron dysregulation is a concern. Here the transferrin receptor interacts with proteins like Amyloid precursor protein contributing to disease pathology through improper metal homeostasis.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

The protein expressed by the gene TFRC is involved in the cellular uptake of iron via receptor-mediated endocytosis, where the transferrin receptor, bound to its ligand, is internalized into specialized endosomes. Following endosomal acidification, iron is released, and the apotransferrin-receptor complex is recycled to the cell surface, where a return to neutral pH results in the loss of apotransferrin's affinity for its receptor. The hereditary hemochromatosis protein HFE competes with transferrin for binding at an overlapping C-terminal site. TFRC positively regulates T and B cell proliferation through iron uptake. It functions as a lipid sensor influencing mitochondrial fusion by modulating the JNK pathway activity. Low dietary levels of stearate promote JNK pathway activation, leading to HUWE1-mediated ubiquitination and degradation of the mitofusin MFN2, inhibiting mitochondrial fusion. High dietary stearate levels result in TFRC stearoylation, which inhibits JNK pathway activation and MFN2 degradation. Additionally, TFRC acts as a receptor for new-world arenaviruses, including Guanarito, Junin, and Machupo viruses. This supplementary information is collated from multiple sources and compiled automatically.
See full target information TFRC
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Immune network 23:e34 PubMed37670811

2023

Tumor Promoting Function of DUSP10 in Non-Small Cell Lung Cancer Is Associated With Tumor-Promoting Cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Wei,Chin Wen Png,Madhushanee Weerasooriya,Heng Li,Chenchen Zhu,Guiping Chen,Chuan Xu,Yongliang Zhang,Xiaohong Xu
View all publications
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代替製品

Primary Antibodies

AB269745

Anti-Transferrin Receptor antibody [TFRC/1818] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [TFRC/1818] - BSA and Azide free (AB269745)

  • 0
  • 0
Primary Antibodies

AB218544

Anti-Transferrin Receptor antibody [TFRC/1817]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [TFRC/1817] (AB218544)

  • 0
  • 0
Primary Antibodies

AB269756

Anti-Transferrin Receptor antibody [TFRC/1817] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [TFRC/1817] - BSA and Azide free (AB269756)

  • 0
  • 0
Primary Antibodies

AB218326

Anti-Transferrin Receptor antibody [TFRC/1818] - Extracellular domain

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [TFRC/1818] - Extracellular domain (AB218326)

  • 0
  • 0
Primary Antibodies

AB212864

Anti-Transferrin Receptor antibody [66IG10] - BSA and Azide free

Flow Cytometry - Anti-Transferrin Receptor antibody [66IG10] - BSA and Azide free (AB212864)

  • 0
  • 0
Primary Antibodies

AB238060

Anti-Transferrin Receptor antibody [TFRC/1839]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [TFRC/1839] (AB238060)

  • 0
  • 0
Primary Antibodies

AB237911

Anti-Transferrin Receptor antibody [TFRC/1839] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [TFRC/1839] - BSA and Azide free (AB237911)

  • 0
  • 0
Primary Antibodies

AB239251

FITC Anti-Transferrin Receptor antibody [MEM-75]

Flow Cytometry - FITC Anti-Transferrin Receptor antibody [MEM-75] (AB239251)

  • 0
  • 0
Primary Antibodies

AB9179

Anti-Transferrin Receptor antibody [MEM-75]

Flow Cytometry - Anti-Transferrin Receptor antibody [MEM-75] (AB9179)

  • 5
  • 1
Primary Antibodies

AB1086

Anti-Transferrin Receptor antibody [MEM-189]

Flow Cytometry - Anti-Transferrin Receptor antibody [MEM-189] (AB1086)

  • 5
  • 4
Primary Antibodies

AB28116

Biotin Anti-Transferrin Receptor antibody [MEM-75]

  • 0
  • 0
Primary Antibodies

AB64672

APC Anti-Transferrin Receptor antibody [MEM-75]

  • 0
  • 0
Primary Antibodies

AB187777

Alexa Fluor® 647 Anti-Transferrin Receptor antibody [MEM-75]

Flow Cytometry - Alexa Fluor® 647 Anti-Transferrin Receptor antibody [MEM-75] (AB187777)

  • 0
  • 0
Primary Antibodies

AB233254

APC/Cy7® Anti-Transferrin Receptor antibody [MEM-75]

Flow Cytometry - APC/Cy7® Anti-Transferrin Receptor antibody [MEM-75] (AB233254)

  • 0
  • 0
Primary Antibodies

AB10249

Anti-Transferrin Receptor antibody [11F5]

  • 0
  • 0

組合せ製品

Assay Kits

AB83366

Iron Assay Kit (Colorimetric)

Biochemical assay - Iron Assay Kit (Colorimetric) (AB83366)

  • 4
  • 3
Primary Antibodies

AB125066

Anti-Glutathione Peroxidase 4 antibody [EPNCIR144]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (AB125066)

  • 5
  • 13
Primary Antibodies

AB155282

Anti-FACL4 antibody [EPR8640]

RabMAb|Recombinant

Western blot - Anti-FACL4 antibody [EPR8640] (AB155282)

  • 4
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB75973

Anti-Ferritin heavy chain antibody [EPR3004Y]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Ferritin heavy chain antibody [EPR3004Y] (AB75973)

  • 4
  • 7

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