Anti-Transferrin Receptor 抗体 (ab84036)
Key features and details
- Rabbit polyclonal to Transferrin Receptor
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Transferrin Receptor antibody
Transferrin Receptor 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Transferrin Receptor -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Dog, Pig, Orangutan
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免疫原
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ポジティブ･コントロール
- ICC/IF: HeLa cells, WB: HeLa, Jurkat and U-2 OS whole cell lysates; Mouse spleen tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98.98% PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84036の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (9) |
Use a concentration of 1 µg/ml. Detects a band of approximately 98 kDa (predicted molecular weight: 84 kDa).
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| ICC/IF | (6) |
Use a concentration of 5 µg/ml.
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| 特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 98 kDa (predicted molecular weight: 84 kDa). |
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ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus. -
関連疾患
Immunodeficiency 46 -
配列類似性
Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain. -
翻訳後修飾
N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. -
細胞内局在
Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 7037 Human
- Entrez Gene: 22042 Mouse
- Entrez Gene: 100171944 Orangutan
- Omim: 190010 Human
- SwissProt: P02786 Human
- SwissProt: Q62351 Mouse
- SwissProt: Q5RDH6 Orangutan
- Unigene: 529618 Human
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別名
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
see all
画像
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Western blot - Anti-Transferrin Receptor antibody (ab84036)All lanes : Anti-Transferrin Receptor antibody (ab84036) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) whole cell lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) whole cell lysate
Lane 3 : U-2 OS (Human osteosarcoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Transferrin Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Western blot - Anti-Transferrin Receptor antibody (ab84036)Anti-Transferrin Receptor antibody (ab84036) at 1 µg/ml + Mouse spleen tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Transferrin Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody (ab84036)ab84036 staining Transferrin receptor protein 1 in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab84036 at 5 µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (200)
ab84036 は 200 報の論文で使用されています。
- Wang YH et al. Dapagliflozin Alleviates Diabetic Kidney Disease via Hypoxia Inducible Factor 1α/Heme Oxygenase 1-Mediated Ferroptosis. Antioxid Redox Signal 40:492-509 (2024). PubMed: 37276148
- Kang L et al. Sevoflurane Exposure Induces Neuronal Cell Ferroptosis Initiated by Increase of Intracellular Hydrogen Peroxide in the Developing Brain via ER Stress ATF3 Activation. Mol Neurobiol 61:2313-2335 (2024). PubMed: 37874483
- Zhang L et al. Fucoxanthin ameliorates traumatic brain injury by suppressing the blood-brain barrier disruption. iScience 26:108270 (2023). PubMed: 37965135
- Kang DY et al. Anticancer Effects of 6-Gingerol through Downregulating Iron Transport and PD-L1 Expression in Non-Small Cell Lung Cancer Cells. Cells 12:N/A (2023). PubMed: 37998363
- Ray A et al. Regulated dynamic subcellular GLUT4 localization revealed by proximal proteome mapping in human muscle cells. J Cell Sci 136:N/A (2023). PubMed: 38126809