AB193188

Anti-Transferrin Receptor 抗体

Name: Anti-Transferrin Receptor 抗体 (ab193188) | Abcam
Brand: Abcam Limited
SKU: AB193188
Price: 60000 JPY
Availability: InStock

Anti-Transferrin Receptor antibody

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(2 Publications)

Rabbit Polyclonal Transferrin Receptor antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TFRC aa 50 to C-terminus.

別名を表示する

CD71, Transferrin receptor protein 1, TR, TfR, TfR1, Trfr, T9, p90, TFRC

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody (AB193188)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody (AB193188)

Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling Transferrin Receptor with ab193188 at a dilution of 1/100. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody (AB193188)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody (AB193188)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue labelling Transferrin Receptor with ab193188 at a dilution of 1/100.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody (AB193188)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling Transferrin Receptor with ab193188 at a dilution of 1/100.

Supplier Data

Western blot - Anti-Transferrin Receptor antibody (AB193188)

All lanes:

Western blot - Anti-Transferrin Receptor antibody (ab193188) at 3 µg/mL

All lanes:

Jurkat whole cell lysate

Secondary

All lanes:

Polyclonal goat anti-rabbit IgG at 1/50000 dilution

Predicted band size: 84 kDa

false

Supplier Data

Western blot - Anti-Transferrin Receptor antibody (AB193188)

All lanes:

Western blot - Anti-Transferrin Receptor antibody (ab193188) at 2 µg/mL

Lane 1:

U251 whole cell lysate

Lane 2:

293T whole cell lysate

Secondary

All lanes:

Polyclonal goat anti-rabbit at 1/10000 dilution

Predicted band size: 84 kDa

Observed band size: 85 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human TFRC aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P02786

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

精製に関する特記事項

>95%

バッファー組成

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The transferrin receptor commonly referred to as TfR or CD71 is an integral membrane protein that facilitates the uptake of transferrin-bound iron into cells. This receptor has a molecular weight of around 95 kDa and often exists as a homodimer on the cell surface. It is widely expressed in many tissues especially in erythroid precursors and rapidly dividing cells. Alternate names for this receptor include TfR1 and TfR2 though they have distinct roles and distributions. Other transmembrane proteins like OX26 and MEM have been studied in relation to the transferrin receptor due to their involvement in drug delivery.

Biological function summary

TfR plays a critical role in iron homeostasis by mediating the internalization of transferrin and release of iron in the endosomes. It operates as part of the transferrin-transferrin receptor complex facilitating iron assimilation necessary for DNA synthesis and cell growth. Iron release involves acidifying endosomes allowing transferrin to bind with specific cellular receptors including alternate forms like beta 2 transferrin. The process subsequently contributes to erythropoiesis and various metabolic processes by regulating essential cellular iron levels.

Pathways

The transferrin receptor is central to iron metabolism and the receptor-mediated endocytosis pathway. It tightly interacts with transferrin and intracellular pathways process the iron released from transferrin within endosomes. The receptor's role in this pathway involves a dynamic with other proteins such as HFE and hepcidin. These interactions help control systemic iron levels linking closely to the maintenance of erythroid cell health and proliferation.

Disruptions in transferrin receptor function correlate with anemia and neurodegenerative disorders. In anemia related to iron deficiency impaired TfR activity reduces iron uptake culminating in insufficient erythropoiesis. Altered receptor expression or function also connects to neurological diseases like Alzheimer's where iron dysregulation is a concern. Here the transferrin receptor interacts with proteins like Amyloid precursor protein contributing to disease pathology through improper metal homeostasis.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

The protein expressed by the gene TFRC is involved in the cellular uptake of iron via receptor-mediated endocytosis, where the transferrin receptor, bound to its ligand, is internalized into specialized endosomes. Following endosomal acidification, iron is released, and the apotransferrin-receptor complex is recycled to the cell surface, where a return to neutral pH results in the loss of apotransferrin's affinity for its receptor. The hereditary hemochromatosis protein HFE competes with transferrin for binding at an overlapping C-terminal site. TFRC positively regulates T and B cell proliferation through iron uptake. It functions as a lipid sensor influencing mitochondrial fusion by modulating the JNK pathway activity. Low dietary levels of stearate promote JNK pathway activation, leading to HUWE1-mediated ubiquitination and degradation of the mitofusin MFN2, inhibiting mitochondrial fusion. High dietary stearate levels result in TFRC stearoylation, which inhibits JNK pathway activation and MFN2 degradation. Additionally, TFRC acts as a receptor for new-world arenaviruses, including Guanarito, Junin, and Machupo viruses. This supplementary information is collated from multiple sources and compiled automatically.

See full target information TFRC

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of traditional and complementary medicine 15:170-181 PubMed40060150

2025

Curcumol promotes ferroptosis of colon cancer by targeting the ubiquitination and degradation of GPX4.

Applications

Unspecified application

Species

Unspecified reactive species

Wuxia Zhao,Qiuying Yan,Lianfang Liu,Dahai Hou,Dongyang Xiang,Dongxin Tang,Liu Li,Weixing Shen,Weiwei Tao,Haibo Cheng,Dongdong Sun

Allergy, asthma, and clinical immunology : officia 16:30 PubMed32351585

2020

Local eosinophils are associated with increased IgA subclass levels in the sinonasal mucosa of chronic rhinosinusitis with polyp patients.

Applications

Unspecified application

Species

Unspecified reactive species

Hossein Aazami,Farhad Seif,Babak Ghalehbaghi,Pegah Babaheidarian,Alireza Mohebbi,Aslan Ahmadi,Majid Khoshmirsafa,Sahand Ghalehbaghi,Babak Behnam,Kobra Zinat Entezami,Zahra Madjd,Reza Falak

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Transferrin Receptor 抗体

Anti-Transferrin Receptor antibody

Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody (AB193188)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody (AB193188)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody (AB193188)

Western blot - Anti-Transferrin Receptor antibody (AB193188)

Western blot - Anti-Transferrin Receptor antibody (AB193188)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

代替製品

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組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise