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リコンビナント

Anti-Transferrin Receptor 抗体 [2/Transferrin] (BSA and Azide free) (ab300658)

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Western blot - Anti-Transferrin Receptor antibody [2/Transferrin] (BSA and Azide free) (AB300658)
  • Western blot - Anti-Transferrin Receptor antibody [2/Transferrin] (BSA and Azide free) (AB300658)
  • Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [2/Transferrin] (BSA and Azide free) (ab300658)
  • Anti-Transferrin Receptor antibody [2/Transferrin] (BSA and Azide free) (AB300658)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [2/Transferrin] to Transferrin Receptor - BSA and Azide free
  • Suitable for: ICC/IF, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Transferrin Receptor antibody [2/Transferrin] (BSA and Azide free)
    Transferrin Receptor 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [2/Transferrin] to Transferrin Receptor - BSA and Azide free

  • 由来種

    Mouse

  • アプリケーション

    適用あり: ICC/IF, WBmore details

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa, K-562, Jurkat, U-87 MG, SW480, SW620, LoVo, HT-29 whole cell lysates; Human placenta and thymus tissue lysates. ICC: K-562.

  • 特記事項

    ab300658 is a carrier free version of ab300657. 

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300658の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
特記事項
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).

ターゲット情報

  • 機能

    Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
    (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.

  • 関連疾患

    Immunodeficiency 46

  • 配列類似性

    Belongs to the peptidase M28 family. M28B subfamily.
    Contains 1 PA (protease associated) domain.

  • 翻訳後修飾

    N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
    Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
    Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.

  • 細胞内局在

    Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD 71 antibody
      • CD71 antibody
      • CD71 antigen antibody
      • IMD46 antibody
      • OTTHUMP00000208523 antibody
      • OTTHUMP00000208524 antibody
      • OTTHUMP00000208525 antibody
      • p90 antibody
      • sTfR antibody
      • T9 antibody
      • TFR 1 antibody
      • TfR antibody
      • TfR1 antibody
      • TFR1_HUMAN antibody
      • TFRC antibody
      • TR antibody
      • Transferrin receptor (p90 CD71) antibody
      • Transferrin receptor protein 1, serum form antibody
      • Trfr antibody
      see all

    画像

    • Western blot - Anti-Transferrin Receptor antibody [2/Transferrin] (BSA and Azide free) (AB300658)
      Western blot - Anti-Transferrin Receptor antibody [2/Transferrin] (BSA and Azide free) (AB300658)
      All lanes : Anti-Transferrin Receptor antibody [2/Transferrin] (ab300657) at 1/1000 dilution

      Lane 1 : Human placenta tissue lysate
      Lane 2 : HeLa (human epithelial cell line from cervical adenocarcinoma), whole cell lysate
      Lane 3 : K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
      Lane 4 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
      Lane 5 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
      Lane 6 : SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution

      Predicted band size: 84 kDa
      Observed band size: 84 kDa



      This data was developed using ab300657, the same antibody clone in a different buffer formulation.

      Blocking / Diluting buffer and concentration: 5% NFDM/TBST

      Exposure time:

      Lane 1: 15 seconds
      Lane 2-4: 46 seconds
      Lane 5, 6: 70 seconds

    • Western blot - Anti-Transferrin Receptor antibody [2/Transferrin] (BSA and Azide free) (AB300658)
      Western blot - Anti-Transferrin Receptor antibody [2/Transferrin] (BSA and Azide free) (AB300658)
      All lanes : Anti-Transferrin Receptor antibody [2/Transferrin] (ab300657) at 1/1000 dilution

      Lane 1 : Human placenta tissue lysate
      Lane 2 : Human thymus tissue lysate
      Lane 3 : SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
      Lane 4 : SW620 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
      Lane 5 : LoVo (human colorectal adenocarcinoma epithelial cell), whole cell lysate
      Lane 6 : HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution

      Predicted band size: 84 kDa
      Observed band size: 84 kDa


      Exposure time: 70 seconds


      This data was developed using ab300657, the same antibody clone in a different buffer formulation.

      Blocking / Diluting buffer and concentration: 5% NFDM/TBST

      Negative controls: thymus (PMID: 29291011).
      Low expression: LoVo (PMID: 31372038).

    • Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [2/Transferrin] (BSA and Azide free) (ab300658)
      Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [2/Transferrin] (BSA and Azide free) (ab300658)

      This data was developed using ab300657, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 80% Methanol fixed, 0.1% Triton X-100 permeabilized K-562 (human chronic myelogenous leukemia lymphoblasts) labeling Transferrin Receptor with ab300657 at 1/50 dilution followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 (2 μg/ml) dilution (green). Confocal image showing membranous and cytoplasmic staining in K-562 cell line. The nuclear counter stain is DAPI (blue).

      Tubulin is detected with ab179513 Anti-beta Tubulin rabbit monoclonal antibody at 1/200 dilution along with ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution (Red).

      Negative control 1 - ab150080 only (secondary only control) without counterstain primary

      Negative control 2 - ab150117 only (secondary only control) without target primary

    • Anti-Transferrin Receptor antibody [2/Transferrin] (BSA and Azide free) (AB300658)
      Anti-Transferrin Receptor antibody [2/Transferrin] (BSA and Azide free) (AB300658)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300658 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300658 は論文での使用が確認できていません。

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