Anti-Transferrin Receptor 2/TFR2 抗体 (ab80194)
Key features and details
- Rabbit polyclonal to Transferrin Receptor 2/TFR2
- Suitable for: IHC-P, IHC-Fr, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Transferrin Receptor 2/TFR2 antibody
Transferrin Receptor 2/TFR2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Transferrin Receptor 2/TFR2 -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: IHC-P, IHC-Fr, WBmore details
適用なし: ICC/IF -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Transferrin Receptor 2/TFR2 aa 150-250 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available asab87613) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98.98% PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab80194の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| IHC-Fr | (10) |
Use at an assay dependent concentration.
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| WB | (20) |
Use a concentration of 1 µg/ml. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
Abcam recommends using 5% BSA as the blocking agent. |
| 特記事項 |
|---|
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IHC-P
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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IHC-Fr
Use at an assay dependent concentration. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa). Abcam recommends using 5% BSA as the blocking agent. |
追加情報
Is unsuitable for ICC/IF.
ターゲット情報
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機能
Mediates cellular uptake of transferrin-bound iron in a non-iron dependent manner. May be involved in iron metabolism, hepatocyte function and erythrocyte differentiation. -
組織特異性
Predominantly expressed in liver. While the alpha form is also expressed in spleen, lung, muscle, prostate and peripheral blood mononuclear cells, the beta form is expressed in all tissues tested, albeit weakly. -
関連疾患
Defects in TFR2 are a cause of hemochromatosis type 3 (HFE3) [MIM:604250]. HFE3 is a disorder of iron hemostasis resulting in iron overload and has a phenotype indistinguishable from that of hereditary hemochromatosis (HH). HH is characterized by abnormal intestinal iron absorption and progressive increase of total body iron, which results in midlife in clinical complications including cirrhosis, cardiopathy, diabetes, endocrine dysfunctions, arthropathy, and susceptibility to liver cancer. Since the disease complications can be effectively prevented by regular phlebotomies, early diagnosis is most important to provide a normal life expectancy to the affected subjects. -
配列類似性
Belongs to the peptidase M28 family. M28B subfamily. -
細胞内局在
Cell membrane and Cytoplasm. Lacks the transmembrane domain. Probably intracellular. - Information by UniProt
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参照データベース
- Entrez Gene: 7036 Human
- Entrez Gene: 50765 Mouse
- Entrez Gene: 288562 Rat
- Omim: 604720 Human
- SwissProt: Q9UP52 Human
- SwissProt: Q9JKX3 Mouse
- SwissProt: B2GUY2 Rat
- Unigene: 544932 Human
see all -
別名
- HFE 3 antibody
- HFE3 antibody
- MGC126368 antibody
see all
画像
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Western blot - Anti-Transferrin Receptor 2/TFR2 antibody (ab80194)Anti-Transferrin Receptor 2/TFR2 antibody (ab80194) at 1 µg/ml + Human Liver Tissue Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 88 kDa
Observed band size: 89 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa, 98 kDa (possible glycosylated form). We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes -
Western blot - Anti-Transferrin Receptor 2/TFR2 antibody (ab80194)This image is courtesy of an anonymous AbreviewAll lanes : Anti-Transferrin Receptor 2/TFR2 antibody (ab80194) at 1/1000 dilution
All lanes : Rat liver whole tissue lysate
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 88 kDa
Observed band size: 88 kDa
Exposure time: 10 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor 2/TFR2 antibody (ab80194)IHC image of Transferrin staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80194, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab80194 は 6 報の論文で使用されています。
- Ji HZ et al. CXCL8 Promotes Endothelial-to-Mesenchymal Transition of Endothelial Cells and Protects Cells from Erastin-Induced Ferroptosis via CXCR2-Mediated Activation of the NF-κB Signaling Pathway. Pharmaceuticals (Basel) 16:N/A (2023). PubMed: 37765018
- Rosato BE et al. SEC23B Loss-of-Function Suppresses Hepcidin Expression by Impairing Glycosylation Pathway in Human Hepatic Cells. Int J Mol Sci 23:N/A (2022). PubMed: 35163229
- Berezovsky B et al. Effect of Erythropoietin on the Expression of Murine Transferrin Receptor 2. Int J Mol Sci 22:N/A (2021). PubMed: 34360974
- Hu W et al. FTH promotes the proliferation and renders the HCC cells specifically resist to ferroptosis by maintaining iron homeostasis. Cancer Cell Int 21:709 (2021). PubMed: 34965856
- Du J et al. Identification of Frataxin as a regulator of ferroptosis. Redox Biol 32:101483 (2020). PubMed: 32169822
- Naz N et al. Ferroportin-1 is a 'nuclear'-negative acute-phase protein in rat liver: a comparison with other iron-transport proteins. Lab Invest : (2012). WB ; Rat . PubMed: 22469696