Anti-Transferrin 抗体 [HTF-14] (ab769)
Key features and details
- Mouse monoclonal [HTF-14] to Transferrin
- Suitable for: ELISA, RIA, ICC, WB, Functional Studies, Sandwich ELISA, IP, IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Transferrin antibody [HTF-14]
Transferrin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [HTF-14] to Transferrin -
由来種
Mouse -
特異性
This antibody recognizes an epitope located in the N-terminal domain of human transferrin. -
アプリケーション
適用あり: ELISA, RIA, ICC, WB, Functional Studies, Sandwich ELISA, IP, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Pig非交差種: Sheep, Horse, Cow, Dog, Carp
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免疫原
Full length native protein (purified) (Pig).
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ポジティブ・コントロール
- WB: Human transferrin. IHC-P: Human Placenta
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Proprietary Purification -
特記事項(精製)
Purified from ascites by sequential precipitation methods. Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
HTF-14 -
ミエローマ
unknown -
アイソタイプ
IgG1 -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab769の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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RIA |
Use at an assay dependent concentration.
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ICC |
Use at an assay dependent concentration.
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WB | (1) |
Use at an assay dependent concentration.
Works best under non reducing conditions. |
Functional Studies |
Use at an assay dependent concentration.
Effectively blocks the transferrin-transferrin receptor interactions. |
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Sandwich ELISA |
Use a concentration of 1 µg/ml.
For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab9538 as Detection. |
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IP |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 10 µg/ml.
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
RIA
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Works best under non reducing conditions. |
Functional Studies
Use at an assay dependent concentration. Effectively blocks the transferrin-transferrin receptor interactions. |
Sandwich ELISA
Use a concentration of 1 µg/ml. For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab9538 as Detection. |
IP
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. -
組織特異性
Expressed by the liver and secreted in plasma. -
関連疾患
Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. -
配列類似性
Belongs to the transferrin family.
Contains 2 transferrin-like domains. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 7018 Human
- Entrez Gene: 100009267 Rabbit
- Omim: 190000 Human
- SwissProt: P02787 Human
- SwissProt: P09571 Pig
- SwissProt: P19134 Rabbit
- Unigene: 518267 Human
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別名
- Apotransferrin antibody
- Beta 1 metal binding globulin antibody
- Beta-1 metal-binding globulin antibody
see all
画像
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All lanes : Anti-Transferrin antibody [HTF-14] (ab769)
Lane 1 : Human Transferrin – 8 µl (reducing conditions) at 5 µg
Lane 2 : HumanTransferrin – 5 µl (reducing conditions) at 3 µg
Lane 3 : Human Transferrin – 3 µl (reducing conditions) at 1 µg
Lane 4 : Human Transferrin – 3 µl (non reducing conditions) at 1 µg
Lane 5 : Human Transferrin – 5 µl (non reducing conditions) at 3 µg
Lane 6 : Human Transferrin - 8 µl (non reducing conditions) at 5 µgThe sample (commercial human transferrin) (1µg/ml in 1x PBS) was mixed with 4x loading buffer (non reducing) or with Laemmli buffer (reducing) and warmed for 3 minutes.
Volume of sample:5; 3; 1 µl of sample + 3; 2; 2 µl of buffer
SDS Page: 12% AA-gel, U=180V, 90 minutes, volume of loading sample:8; 5;3 µl
Marker – 3 µl
WB: Nitrocelulose membrane (8x5.5 cm), I=35mA, 90minutes
Detection:
Blocking solution: 1xPBS/5% BSA, 4°C, overnight
Primary Ab: membrane was incubated in 5 ml DMEM/10%FTS + 10 µl HTF-14 (primary Ab) (60 minutes, room temperature)
Washing solution: 1x PBS/0.1% Tween
Secondary Ab: IRDye® 800CW Goat anti-mouse IgG1-specific, dilution 1/5000, diluting solution – 5% BSA/1x PBS, incubation for 60 minutes, low temperature.
Detection: Li-COR odyssey
No reducing agent should be added to the sample buffer. The sample can be denatured and processed by common Western blotting protocols, but the disulfide bonds must not be reduced.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [HTF-14] (ab769)
Immunohistochemistry staining of human placenta (paraffin sections) using ab769 10 μg/ml
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab769 は 11 報の論文で使用されています。
- Zhang LJ et al. Age-Related Loss of Innate Immune Antimicrobial Function of Dermal Fat Is Mediated by Transforming Growth Factor Beta. Immunity 50:121-136.e5 (2019). PubMed: 30594464
- Gianneli M et al. Label-free in-flow detection of receptor recognition motifs on the biomolecular corona of nanoparticles. Nanoscale 10:5474-5481 (2018). PubMed: 29511756
- Lu R et al. Spontaneous severe hypercholesterolemia and atherosclerosis lesions in rabbits with deficiency of low-density lipoprotein receptor (LDLR) on exon 7. EBioMedicine 36:29-38 (2018). PubMed: 30243490
- Griveau A et al. A Glial Signature and Wnt7 Signaling Regulate Glioma-Vascular Interactions and Tumor Microenvironment. Cancer Cell 33:874-889.e7 (2018). PubMed: 29681511
- Lo Giudice MC et al. In situ characterization of nanoparticle biomolecular interactions in complex biological media by flow cytometry. Nat Commun 7:13475 (2016). Flow Cyt ; Human . PubMed: 27845346