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AB28751

Anti-TRAK1 抗体

Anti-TRAK1 antibody

1

(2 Reviews)

|

(2 Publications)

Goat Polyclonal TRAK1 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human TRAK1 aa 900 to C-terminus.

別名を表示する

KIAA1042, OIP106, TRAK1, Trafficking kinesin-binding protein 1, 106 kDa O-GlcNAc transferase-interacting protein, Protein Milton

1 Images
Western blot - Anti-TRAK1 antibody (AB28751)
  • WB

Unknown

Western blot - Anti-TRAK1 antibody (AB28751)

Primary incubation was for 1 hour.
Detected by western blot using chemiluminescence.

All lanes:

Western blot - Anti-TRAK1 antibody (ab28751) at 0.3 µg/mL

All lanes:

human heart lysate (RIPA buffer, 30µg total protein per lane)

Predicted band size: 106 kDa

Observed band size: 90 kDa

false

Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

Synthetic Peptide within Human TRAK1 aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UPV9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1.5 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product.</p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
精製に関する特記事項
This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
バッファー組成
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TRAK1 also known as trafficking kinesin-binding protein 1 is a motor protein adaptor with a critical role in intracellular transport. It has an approximate mass of 95 kDa. TRAK1 facilitates the interaction between kinesin motors and their cargo enabling proper transport along microtubules. It expresses widely though high levels can be found in the nervous system. TRAK1 contains specific domains that bind to both kinesin motors and microtubules allowing it to effectively guide intracellular movement.
Biological function summary

TRAK1 influences mitochondrial trafficking within neurons. It is part of a complex with other motor proteins such as kinesin and dynein that manage mitochondria distribution. By regulating this process TRAK1 affects energy supply critical for neuron function. Proper mitochondrial distribution holds importance for neuron survival and health and TRAK1 impacts synaptic plasticity through this mechanism.

Pathways

TRAK1 plays an essential part in the neuronal trafficking pathways. It works closely with the mitochondria transport pathway along with proteins like Miro1 and Miro2 to ensure mitochondrial movement. This ensures proper placement of mitochondria where energy demand is highest. TRAK1's role in the axonal transport pathway also ties it to neuron structure maintenance and repair aligning it closely with kinesin-related pathways.

Aberrations in TRAK1 function have links to neurodegenerative illnesses like Alzheimer's disease. Mutations or dysfunctions in TRAK1 may lead to improper mitochondrial distribution contributing to neuronal injury. It also has a connection to Charcot-Marie-Tooth disease where disruptions in mitochondrial function are important. In these contexts TRAK1 relates to other proteins such as Miro1 and MFN2 impacting their regulation and affecting disease progression.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Involved in the regulation of endosome-to-lysosome trafficking, including endocytic trafficking of EGF-EGFR complexes and GABA-A receptors (PubMed : 18675823). Involved in mitochondrial motility. When O-glycosylated, abolishes mitochondrial motility. Crucial for recruiting OGT to the mitochondrial surface of neuronal processes (PubMed : 24995978). TRAK1 and RHOT form an essential protein complex that links KIF5 to mitochondria for light chain-independent, anterograde transport of mitochondria (By similarity).
See full target information TRAK1
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in aging neuroscience 13:632374 PubMed33762923

2021

Dl-3-n-Butylphthalide Alleviates Demyelination and Improves Cognitive Function by Promoting Mitochondrial Dynamics in White Matter Lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Yiwei Feng,Min Guo,Hongchen Zhao,Sida Han,Yining Hao,Yiwen Yuan,Weiwei Shen,Jian Sun,Qiang Dong,Mei Cui

Human molecular genetics 23:906-19 PubMed24092329

2013

DISC1 complexes with TRAK1 and Miro1 to modulate anterograde axonal mitochondrial trafficking.

Applications

ICC/IF, WB

Species

Human, Human

Fumiaki Ogawa,Elise L V Malavasi,Darragh K Crummie,Jennifer E Eykelenboom,Dinesh C Soares,Shaun Mackie,David J Porteous,J Kirsty Millar
View all publications
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組合せ製品

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Primary Antibodies

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Secondary Antibodies

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