Anti-TRAF3 抗体 (ab36988)
Key features and details
- Rabbit polyclonal to TRAF3
- Suitable for: IP, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-TRAF3 antibody
TRAF3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TRAF3 -
由来種
Rabbit -
アプリケーション
適用あり: IP, ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
A 15 amino acid peptide from near the amino terminus of human TRAF3.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab36988の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP | (1) |
Use at an assay dependent concentration.
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
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| 特記事項 |
|---|
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IP
Use at an assay dependent concentration. |
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ICC/IF
Use a concentration of 10 µg/ml. |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). |
ターゲット情報
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機能
Regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of B cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa-B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14. -
配列類似性
Belongs to the TNF receptor-associated factor family. A subfamily.
Contains 1 MATH domain.
Contains 1 RING-type zinc finger.
Contains 2 TRAF-type zinc fingers. -
ドメイン
The MATH/TRAF domain binds to receptor cytoplasmic domains.
The Ring-type zinc finger domain is required for its function in down-regulation of NFKB2 proteolytic processing. -
翻訳後修飾
Undergoes 'Lys-48'-linked polyubiquitination, leading to its proteasomal degradation in response to signaling by TNFSF13B, TLR4 or through CD40. Undergoes 'Lys-63'-linked ubiquitination during early stages of virus infection, and 'Lys-48'-linked ubiquitination during later stages. Undergoes both 'Lys-48'-linked and 'Lys-63'-linked ubiquitination in response to TLR3 and TLR4 signaling (By similarity). Deubiquitinated by OTUB1, OTUB2 and OTUD5. -
細胞内局在
Cytoplasm. Endosome. Mitochondrion. Undergoes endocytosis together with TLR4 upon LPS signaling (By similarity). Associated with mitochondria in response to virus. - Information by UniProt
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参照データベース
- Entrez Gene: 7187 Human
- Entrez Gene: 22031 Mouse
- Entrez Gene: 362788 Rat
- Omim: 601896 Human
- SwissProt: Q13114 Human
- SwissProt: Q60803 Mouse
- Unigene: 510528 Human
- Unigene: 27431 Mouse
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別名
- CAP 1 antibody
- CAP-1 antibody
- CAP1 antibody
see all
画像
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Western blot - Anti-TRAF3 antibody (ab36988)Lane 1 : Anti-TRAF3 antibody (ab36988) at 2 µg/ml
Lane 2 : Anti-TRAF3 antibody (ab36988) at 4 µg/ml
All lanes : Hela cell lysate.
Predicted band size: 65 kDa
Observed band size: 65 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-TRAF3 antibody (ab36988)ab36988 at 10µg/ml staining TRAF3 in Hela cells by ICC/IF
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab36988 は 15 報の論文で使用されています。
- Zhang X et al. Extracellular fibrinogen-binding protein released by intracellular Staphylococcus aureus suppresses host immunity by targeting TRAF3. Nat Commun 13:5493 (2022). PubMed: 36123338
- Chang W et al. Downregulation of deubiquitinating enzyme USP25 promotes the development of allergic rhinitis by enhancing TSLP signaling in the nasal epithelium. Mol Med Rep 26:N/A (2022). PubMed: 36177892
- Ji W et al. Triptolide attenuates inhibition of ankylosing spondylitis-derived mesenchymal stem cells on the osteoclastogenesis through modulating exosomal transfer of circ-0110634. J Orthop Translat 36:132-144 (2022). PubMed: 36185580
- Yang Z et al. miR-155-5p in Extracellular Vesicles Derived from Choroid Plexus Epithelial Cells Promotes Autophagy and Inflammation to Aggravate Ischemic Brain Injury in Mice. Oxid Med Cell Longev 2022:8603427 (2022). PubMed: 35222806
- Wang YL et al. Hepatoprotective effects of sevoflurane against hepatic ischemia-reperfusion injury by regulating microRNA-124-3p-mediated TRAF3/CREB axis. Cell Death Discov 8:105 (2022). PubMed: 35260558