Anti-TRAF1 抗体 [EPR26204-112] (BSA and Azide free) (ab300076)

製品名

Anti-TRAF1 antibody [EPR26204-112] (BSA and Azide free)
TRAF1 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR26204-112] to TRAF1 - BSA and Azide free
由来種

Rabbit
アプリケーション

適用あり: IP, IHC-P, WBmore details
適用なし: Flow Cyt or ICC/IF
種交差性

交差種: Mouse, Rat, Human
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: Whole cell fresh lysates Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysates of: Ramos (human Burkitt's lymphoma B lymphocyte), Daudi (human Burkitt's lymphoma lymphoblast), Daudi whole cell fresh lysate, human tonsil and lymphoma tissue lysate.
特記事項
ab300076 is a cvarrier free version of ab300075.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C.
バッファー

pH: 7.2
Constituent: 100% PBS
キャリア・フリー

はい
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR26204-112
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-TRAF1 antibody [EPR26204-112] (ab300075)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab300076の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
IP		Use at an assay dependent concentration.
IHC-P		Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB		Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 46 kDa).

特記事項
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 46 kDa).

追加情報

Is unsuitable for Flow Cyt or ICC/IF.

機能

Adapter molecule that regulates the activation of NF-kappa-B and JNK. Plays a role in the regulation of cell survival and apoptosis. The heterotrimer formed by TRAF1 and TRAF2 is part of a E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein-ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2.
配列類似性

Contains 1 MATH domain.
ドメイン

The coiled coil domain mediates homo- and hetero-oligomerization.
The MATH/TRAF domain binds to receptor cytoplasmic domains.
Cleavage by CASP8 liberates a C-terminal fragment that promotes apoptosis and inhibits the activation of NF-kappa-B in response to TNF signaling.
翻訳後修飾

Polyubiquitinated by BIRC2 and/or BIRC3, leading to its subsequent proteasomal degradation.
Target information above from: UniProt accession Q13077 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 7185 Human
- Entrez Gene: 22029 Mouse
- Entrez Gene: 687813 Rat
- Omim: 601711 Human
- SwissProt: Q13077 Human
- SwissProt: P39428 Mouse
- Unigene: 531251 Human
- Unigene: 239514 Mouse
別名
- 4732496E14Rik antibody
- EBI6 antibody
- EBV induced protein 6 antibody
- Epstein-Barr virus-induced protein 6 antibody
- MGC:10353 antibody
- Tnf receptor associated factor 1 antibody
- TNF receptor-associated factor 1 antibody
- TRAF 1 antibody
- TRAF1 antibody
- TRAF1_HUMAN antibody
see all

Immunoprecipitation - Anti-TRAF1 antibody [EPR26204-112] (BSA and Azide free) (AB300076)

This data was developed using ab300075, the same antibody clone in a different buffer formulation.

TRAF1 was immunoprecipitated from 0.35 mg Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 µg with ab300075 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300075 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 µg

Lane 2: ab300075 in Raji whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300075 in Raji whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 10 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (BSA and Azide free) (AB300076)

This data was developed using ab300075, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labelling TRAF1 with ab300075 at 1/100 (5.64 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Cytoplasmic staining on megakaryocytes in rat spleen (PMID: 11046039). The section was incubated with ab300075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) kit.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (BSA and Azide free) (AB300076)

This data was developed using ab300075, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling TRAF1 with ab300075 at 1/100 (5.64 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Cytoplasmic staining on megakaryocytes in mouse spleen (PMID: 11046039). The section was incubated with ab300075 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (BSA and Azide free) (AB300076)

This data was developed using ab300075, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse thymus tissue labelling TRAF1 with ab300075 at 1/100 (5.64 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Cytoplasmic staining on mouse thymus medulla (PMID: 11046039). The section was incubated with ab300075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (BSA and Azide free) (AB300076)

This data was developed using ab300075, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human thymus tissue labelling TRAF1 with ab300075 at 1/1000 (0.564 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Cytoplasmic staining on human thymus (PMID: 11046039). The section was incubated with ab300075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (BSA and Azide free) (AB300076)

This data was developed using ab300075, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human lymph node hyperplasia tissue labelling TRAF1 with ab300075 at 1/1000 (0.564 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Cytoplasmic staining on human lymph node hyperplasia (PMID: 11046039). The section was incubated with ab300075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (BSA and Azide free) (AB300076)

This data was developed using ab300075, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labelling TRAF1 with ab300075 at 1/1000 (0.564 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) was used. Cytoplasmic staining on Reed-Sternberg cells in human Hodgkin's lymphoma (PMID: 12502848). The section was incubated with ab300075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
Western blot - Anti-TRAF1 antibody [EPR26204-112] (BSA and Azide free) (AB300076)

All lanes : Anti-TRAF1 antibody [EPR26204-112] (ab300075) at 1/1000 dilution

Lane 1 : Raji (human Burkitt's lymphoma B lymphocyte) whole cell fresh lysate
Lane 2 : HEK-293 (human embryonic kidney epithelial cell) whole cell fresh lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 46 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?

Exposure time: 15 seconds

This data was developed using ab300075, the same antibody clone in a different buffer formulation.

5% NFDM/TBST was used as blocking and iluting buffer.

Lysates were freshly made and used in WB test immediately to minimize protein degradation.
The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 25321474, 25996949).
Negative control: HEK-293 (PMID: 12502848)
Western blot - Anti-TRAF1 antibody [EPR26204-112] (BSA and Azide free) (AB300076)

All lanes : Anti-TRAF1 antibody [EPR26204-112] (ab300075) at 1/1000 dilution

Lane 1 : Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate
Lane 3 : Daudi whole cell fresh lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 46 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?

This data was developed using ab300075, the same antibody clone in a different buffer formulation.

5% NFDM/TBST was used as blocking and iluting buffer.

Exposure time:

Lane 1-2: 92 seconds
Lane3: 3 minutes

Left-hand blot (lanes 1 & 2) was developed using a high sensitivity ECL substrate.
Lane 3: Lysates were freshly made and used in WB test immediately to minimize protein degradation.
The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 25321474, 25996949).
Western blot - Anti-TRAF1 antibody [EPR26204-112] (BSA and Azide free) (ab300076)

All lanes : Anti-TRAF1 antibody [EPR26204-112] (ab300075) at 1/1000 dilution

Lane 1 : Human tonsil tissue lysate
Lane 2 : Human lymphoma tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 46 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?

Exposure time: 92 seconds

This data was developed using ab300075, the same antibody clone in a different buffer formulation.

5% NFDM/TBST was used as blocking and diluting buffer.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 25321474, 25996949).
Anti-TRAF1 antibody [EPR26204-112] (BSA and Azide free) (AB300076)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet download

Download

ab300076 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab300076 は 1 報の論文で使用されています。

Hong Y et al. Elabela inhibits TRAF1/NF-κB induced oxidative DNA damage to promote diabetic foot ulcer wound healing. iScience 26:107601 (2023). PubMed: 37664606

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製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

キャリア・フリー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

ドメイン

翻訳後修飾

参照データベース

別名

画像

プロトコール

データシートおよび資料

Datasheet download

Certificate of Compliance

参考文献 (1)

レビューと Q&A