AB72625

Anti-TRA2A 抗体

Name: Anti-TRA2A 抗体 (ab72625) | Abcam
Brand: Abcam Limited
SKU: AB72625
Price: 75000 JPY
Availability: InStock
Rating: 5 (1 reviews)

Anti-TRA2A antibody

(1 Review)

(3 Publications)

Rabbit Polyclonal TRA2A antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TRA2A.

別名を表示する

Transformer-2 protein homolog alpha, TRA-2 alpha, TRA2-alpha, Transformer-2 protein homolog A, TRA2A

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRA2A antibody (AB72625)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRA2A antibody (AB72625)

Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab72625 at 1 : 50 dilution.
Left image un-treated.
Right image treated with immunizing peptide.

Immunocytochemistry/ Immunofluorescence - Anti-TRA2A antibody (AB72625)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TRA2A antibody (AB72625)

Immunofluorescence analysis of Hela cells using ab72625 at a 1 : 500 dilution.
Left image un-treated.
Right image treated with immunizing peptide.

AbReview39331****

Western blot - Anti-TRA2A antibody (AB72625)

All lanes:

Western blot - Anti-TRA2A antibody (ab72625) at 1/250 dilution

Lanes 1, 3 and 5:

293T whole cell lysate at 5 µg

Lanes 2, 4 and 6:

Mouse ES (129Sv) whole cell lysate at 5 µg

Secondary

All lanes:

HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/5000 dilution

Predicted band size: 33 kDa

Observed band size: 27 kDa,33 kDa,85 kDa

false

Exposure time: 1min

This image is courtesy of an anonymous Abreview

Unknown

Western blot - Anti-TRA2A antibody (AB72625)

All lanes:

Western blot - Anti-TRA2A antibody (ab72625) at 1/500 dilution

Lane 1:

Extracts from HUVEC cells at 5 µg

Lane 2:

Extracts from HUVEC cells plus 5µg immunizing peptide at 5 µg

Predicted band size: 33 kDa

Observed band size: 27 kDa,33 kDa,44 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, IHC-P, WB

applications

免疫原

Synthetic Peptide within Human TRA2A. The exact immunogen used to generate this antibody is proprietary information.

Q13595

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TRA2A also known as transformer 2 alpha homolog is an RNA-binding protein involved in numerous cellular processes. This protein has a molecular weight of approximately 31 kDa. It is expressed in many tissues including the brain and testes. TRA2A binds to specific RNA sequences modulating splicing events and affecting the choice of splice sites. It belongs to the serine/arginine-rich protein family which plays key roles in the regulation of both alternative and constitutive splicing.

Biological function summary

TRA2A influences gene expression and transcript variants by engaging in complex interactions. It acts as part of the spliceosome machinery which is a large ribonucleoprotein complex responsible for splicing pre-mRNA. TRA2A ensures precise removal of introns and ligation of exons producing different mRNA isoforms. These isoforms translate into functionally distinct proteins influencing cellular activity.

Pathways

TRA2A participates in the mRNA processing pathway influencing genetic output by regulating splice site selection. TRA2A connects to the MAPK signaling pathway as it is known to interact with proteins like SAM68 which also affects mRNA splicing. This connectivity with other proteins highlights the regulatory network's complexity ensuring accurate gene expression profiles.

TRA2A has been implicated in cancer and neurodegenerative disorders. Altered expression and splicing activity of TRA2A contribute to oncogenesis as seen in breast cancer where abnormal splicing can drive tumor progression. TRA2A also impacts neurological conditions interplaying with proteins like tau in Alzheimer's disease where aberrant splicing plays a part in disease pathology. Understanding these links offers potential therapeutic angles for targeting TRA2A-related pathways.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing.

See full target information TRA2A

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 12:4883-4890 PubMed34234858

2021

TRA2A Binds With LncRNA MALAT1 To Promote Esophageal Cancer Progression By Regulating EZH2/β-catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Zhao,Qiuyang Chen,Yujie Cai,Danze Chen,Mingrong Bei,Hongyan Dong,Jianzhen Xu

Cell reports 25:3422-3434.e7 PubMed30566867

2018

An Integrative Study of Protein-RNA Condensates Identifies Scaffolding RNAs and Reveals Players in Fragile X-Associated Tremor/Ataxia Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Fernando Cid-Samper,Mariona Gelabert-Baldrich,Benjamin Lang,Nieves Lorenzo-Gotor,Riccardo Delli Ponti,Lies-Anne W F M Severijnen,Benedetta Bolognesi,Ellen Gelpi,Renate K Hukema,Teresa Botta-Orfila,Gian Gaetano Tartaglia

Life science alliance 1:e201800070 PubMed30456350

2018

Proteomics and neuropathology identify ribosomes as poly-GR/PR interactors driving toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Hannelore Hartmann,Daniel Hornburg,Mareike Czuppa,Jakob Bader,Meike Michaelsen,Daniel Farny,Thomas Arzberger,Matthias Mann,Felix Meissner,Dieter Edbauer

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRA2A 抗体

Anti-TRA2A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRA2A antibody (AB72625)

Immunocytochemistry/ Immunofluorescence - Anti-TRA2A antibody (AB72625)

Western blot - Anti-TRA2A antibody (AB72625)

Western blot - Anti-TRA2A antibody (AB72625)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise