Anti-TPX2 抗体 [18D5-1] (ab32795)

OK, thanks. If it has never worked, with or without boiling the samples, and using the conditions you sent earlier, I think it should be replaced. For a replacement, we do not have other antibodies that have been validated for reactivity with mouse samples. I suggest ab71816: Click here (or use the following: https://www.abcam.com/TPX2-antibody-ab71816.html). As I mentioned, if you would like an antibody against something else, I can replace ab32795 with that instead. I look forward to your reply.

Thank you for sending the details of your sample preparation. Have you stained blots of these samples for any other proteins? I think the preparation is good, though we recommend boiling samples to ensure complete denaturation. It is possible that the epitope this monoclonal recognizes on TPX2 is inaccessible otherwise. On the other hand, the antibody is presumably capable of staining the protein in its native conformation, given the ICC and IHC data, so incomplete denaturation may not be the issue. It would not, in any case, account for the large number of non-specific bands. My one other concern is whether TPX2 is present in your samples. I am not sure whether expression is tissue-specific, but a positive control of mouse 3T3 cells may help clarify. (The datasheet includes a stain of a 3T3 cell culture). Given the non-specific banding, I suggest we replace the antibody with another, if you are interested. We do not have another TPX2 antibody that has been validated for mouse samples but the antibody ab71816 looks somewhat promising in that the western blot data on our datasheet shows a band at the expected molecular weight of 86 kD, and the immunogen has some homology with mouse TPX2. We can also issue a credit or refund instead, or we can replace the TPX2 antibody with an antibody against a different protein.

Thank you for contacting Abcam about this TPX2 western blot issue and for sending the image of the blot. Can you please give me a few details about the samples and the protocol? In particular, we are interested in the species and the nature of the sample (cell line or tissue type), how the samples were prepared, the membrane blocking solution, and the antibody concentration. I assume all the other reagents, including the secondary antibody, are effective with other antibodies. We have received a few complaints over the past year, but it is not clear if these are specific for one lot. If your protocol and sample preparation are standard, as I expect they are, then I think it will be best to replace the antibody with a different antibody. My suggestion will depend on the species of your sample. When you send your sample and protocol details, coule you please also send the PO number or approzimate date of your order?