AB283263

Anti-TPOR/MPL 抗体 [EPR24566-47]

Anti-TPOR/MPL antibody [EPR24566-47]

20ul selling size
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal TPOR/MPL antibody. Suitable for Flow Cyt, WB and reacts with Human samples.

別名を表示する

CD110, TPOR, MPL, Thrombopoietin receptor, TPO-R, Myeloproliferative leukemia protein, Proto-oncogene c-Mpl

2 Images

Flow Cytometry - Anti-TPOR/MPL antibody [EPR24566-47] (AB283263)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-TPOR/MPL antibody [EPR24566-47] (AB283263)

Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling TPOR/MPL with ab283263 at 1/500 dilution (0.1 μg) (Right) compared with a Rabbit monoclonal IgG isotype control (ab172730) (Left). Goat anti rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab283263, then stained with anti-CD41 conjugated to APC.

Gated on viable cells.

Lab

Western blot - Anti-TPOR/MPL antibody [EPR24566-47] (AB283263)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 19880496, 7689867, 23183425).

Negative control : Jurkat (PMID : 7689867, 23183425); K-562 (PMID : 7689867, 23183425).

Exposure time : 59 seconds.

All lanes:

Western blot - Anti-TPOR/MPL antibody [EPR24566-47] (ab283263) at 1/1000 dilution

Lane 1:

HEL (human erythroleukemia erythroblast) whole cell lysate at 20 µg

Lane 2:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Lane 3:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 71 kDa

Observed band size: 80 kDa

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Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR24566-47

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The target TPOR/MPL also known as the MPL protein or Myeloproliferative Leukemia protein functions as a receptor for thrombopoietin (TPO). It is composed of 635 amino acids with a molecular weight of approximately 70 kDa. This protein is expressed primarily on the surface of hematopoietic progenitor cells megakaryocytes and platelets. The receptor a cell surface-bound protein plays a significant role by binding to TPO facilitating cellular signaling involved in cell proliferation and maturation.

Biological function summary

The TPOR/MPL protein promotes the growth and differentiation of multiple blood cell types through direct interactions with TPO. When TPO binds to MPL it triggers a signaling cascade essential for the regulation of platelet production and maintenance of hematopoiesis. This receptor forms part of a larger receptor complex that includes JAK2 protein kinase which further transduces signals for cell growth and survival. The engagement of JAK2 enhances the functional capability of MPL leading to the activation of various downstream effectors.

Pathways

The TPOR/MPL protein is central to the thrombopoietin signaling pathway facilitating the activation of downstream molecules like STAT3 and STAT5. These transcription factors activate gene expression critical for cell proliferation and survival. MPL is closely related to other hematopoietic receptors within the Jak-STAT pathway. The pathway is integral for hematopoietic stem cell function and involves interactions with proteins like EPO receptor which utilizes similar pathways for erythropoiesis.

The TPOR/MPL protein is implicated in myeloproliferative disorders such as essential thrombocythemia and primary myelofibrosis. Mutations in the MPL gene can alter receptor function leading to uncontrolled cell proliferation. Such mutations often present in tandem with mutations in other proteins like JAK2 amplifying the disease processes. The inappropriate activation of the MPL signaling pathway results in excessive production of platelets or fibrosis within the bone marrow contributing significantly to disease phenotypes.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Receptor for thrombopoietin that regulates hematopoietic stem cell renewal, megakaryocyte differentiation, and platelet formation. Upon activation by THPO, induces rapid tyrosine phosphorylation and activation of JAK2, providing docking sites for many signaling proteins such as STAT5, SHIP/INPP5D, GRB2, SOS1 and PI3K (PubMed : 15899890, PubMed : 37633268). In turn, These signaling cascades lead to the proliferation, survival, and differentiation of megakaryocytes, ultimately leading to increased platelet production.

See full target information MPL

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TPOR/MPL 抗体 [EPR24566-47]

Anti-TPOR/MPL antibody [EPR24566-47]

Flow Cytometry - Anti-TPOR/MPL antibody [EPR24566-47] (AB283263)

Western blot - Anti-TPOR/MPL antibody [EPR24566-47] (AB283263)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

Abcam product promise