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リコンビナントRabMAb

Anti-TPH2 抗体 [EPR19191] (ab184505)

Reviews (1) Submit a question References (18)
Immunohistochemistry (Frozen sections) - Anti-TPH2 antibody [EPR19191] (ab184505)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] (ab184505)
  • Western blot - Anti-TPH2 antibody [EPR19191] (ab184505)
  • Immunoprecipitation - Anti-TPH2 antibody [EPR19191] (ab184505)
  • Immunohistochemistry (Frozen sections) - Anti-TPH2 antibody [EPR19191] (ab184505)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] (ab184505)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] (ab184505)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] (ab184505)
  • Anti-TPH2 antibody [EPR19191] (ab184505)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19191] to TPH2
  • Suitable for: WB, IHC-P, IP, IHC-Fr
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-TPH2 antibody [EPR19191]
    TPH2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR19191] to TPH2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, IP, IHC-Frmore details
    適用なし: ICC

  • 種交差性

    交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse midbrain and brainstem lysates. IHC-P: Mouse and rat brainstem tissues. IHC-Fr: Mouse brainstem and cerebrum tissue. IP: Mouse brain lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR19191

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184505の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/40.
IHC-Fr
1/500.
特記事項
WB
1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/40.
IHC-Fr
1/500.
追加情報
Is unsuitable for ICC.

ターゲット情報

  • 組織特異性

    Brain specific.

  • パスウェイ

    Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.

  • 関連疾患

    Genetic variation in TPH2 may influence susceptibility to major depressive disorder (MDD) [MIM:608516].
    Defects in TPH2 are the cause of susceptibility to attention deficit-hyperactivity disorder type 7 (ADHD7) [MIM:613003]. ADHD is a neurobehavioral developmental disorder and is primarily characterized by the co-existence of attentional problems and hyperactivity, with each behavior occurring infrequently alone. Note=Naturally occurring variants of TPH2 with impaired enzyme activity could cause deficiency of serotonin production and result in an increased risk of developing behavioral disorders.

  • 配列類似性

    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
    Contains 1 ACT domain.
  • Information by UniProt

  • 参照データベース

    • 別名

      • MGC138871 antibody
      • ADHD7 antibody
      • FLJ37295 antibody
      • MGC138872 antibody
      • Neuronal tryptophan hydroxylase antibody
      • NTPH antibody
      • TPH 2 antibody
      • Tph2 antibody
      • TPH2_HUMAN antibody
      • Tryptophan 5-hydroxylase 2 antibody
      • Tryptophan 5-monooxygenase 2 antibody
      • Tryptophan hydroxylase 2 antibody
      see all

    画像

    • Immunohistochemistry (Frozen sections) - Anti-TPH2 antibody [EPR19191] (ab184505)
      Immunohistochemistry (Frozen sections) - Anti-TPH2 antibody [EPR19191] (ab184505)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling TPH2 with ab184505 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse cerebrum. is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 (2 ug/ml) dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] (ab184505)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] (ab184505)

      Immunohistochemical analysis of paraffin-embedded Mouse brainstem tissue labeling TPH2 with ab184505 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on dorsal raphe cells of mouse brainstem [PMID: 22912815]. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-TPH2 antibody [EPR19191] (ab184505)
      Western blot - Anti-TPH2 antibody [EPR19191] (ab184505)
      All lanes : Anti-TPH2 antibody [EPR19191] (ab184505) at 1/1000 dilution

      Lane 1 : Mouse midbrain lysate
      Lane 2 : Mouse brainstem lysate
      Lane 3 : Mouse kidney lysate
      Lane 4 : Mouse spleen lysate
      Lane 5 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
      Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 56 kDa
      Observed band size: 56 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      This target is specifically expressed in DR (dorsal raphe nucleus). WB was tested with midbrain or brainstem because both include the DR zone. The results were consistent with the literature (PMID:21272616; PMID: 19181488).

    • Immunoprecipitation - Anti-TPH2 antibody [EPR19191] (ab184505)
      Immunoprecipitation - Anti-TPH2 antibody [EPR19191] (ab184505)

      TPH2 was immunoprecipitated from 0.35 mg of Mouse brain lysate with ab184505 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184505 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: Mouse brain lysate, 10µg (Input).

      Lane 2: ab184505 IP in Mouse brain lysate.

      Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab184505 in Mouse brain lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 10 seconds.

    • Immunohistochemistry (Frozen sections) - Anti-TPH2 antibody [EPR19191] (ab184505)
      Immunohistochemistry (Frozen sections) - Anti-TPH2 antibody [EPR19191] (ab184505)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse brainstem tissue labeling TPH2 with ab184505 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasm staining on dorsal raphe cells of mouse brainstem [PMID: 22912815]. The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 secondary antibody at 1/1000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] (ab184505)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] (ab184505)

      Immunohistochemical analysis of paraffin-embedded Rat brainstem tissue labeling TPH2 with ab184505 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on dorsal raphe cells of rat brainstem [PMID: 22912815]. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] (ab184505)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] (ab184505)

      Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling TPH2 with ab184505 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on mouse spleen. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] (ab184505)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] (ab184505)

      Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling TPH2 with ab184505 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on rat stomach. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-TPH2 antibody [EPR19191] (ab184505)
      Anti-TPH2 antibody [EPR19191] (ab184505)

    参考文献 (18)

    ab184505 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab184505 は 18 報の論文で使用されています。

    • Cheung A  et al. Neurexins in serotonergic neurons regulate neuronal survival, serotonin transmission, and complex mouse behaviors. Elife 12:N/A (2023). PubMed: 36695811
    • Svalbe B  et al. Antidepressive-like Behavior-Related Metabolomic Signatures of Sigma-1 Receptor Knockout Mice. Biomedicines 10:N/A (2022). PubMed: 35884876
    • Battaglia DM  et al. Herpes Simplex Virus-1 Induced Serotonin-Associated Metabolic Pathways Correlate With Severity of Virus- and Inflammation-Associated Ocular Disease. Front Microbiol 13:859866 (2022). PubMed: 35391733
    • Rodnyy AY  et al. Serotonin 5-HT7 receptor overexpression in the raphe nuclei area produces antidepressive effect and affects brain serotonin system in male mice. J Neurosci Res 100:1506-1523 (2022). PubMed: 35443076
    • Wang B  et al. Ahi1 regulates serotonin production by the GR/ERβ/TPH2 pathway involving sexual differences in depressive behaviors. Cell Commun Signal 20:74 (2022). PubMed: 35643536
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Western blot abreview for Anti-TPH2 antibody [EPR19191]

     Excellent
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (brain)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    brain
    Blocking step
    Odyssey blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Verified customer

    投稿 Oct 04 2019

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