Anti-TPGS1 抗体 [EPR14324] (ab184178)
![Western blot - Anti-TPGS1 antibody [EPR14324] (ab184178)](https://www.abcam.co.jp/ps/products/184/ab184178/Images/ab184178-216528-anti-tpgs1-antibody-epr14324-western-blot.jpg "Western blot - Anti-TPGS1 antibody [EPR14324] (ab184178)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14324] to TPGS1
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-TPGS1 antibody [EPR14324]
TPGS1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR14324] to TPGS1 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IPmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- Rat testis, Mouse testis or SH-SY5Y cell lysates, SH-SY5Y cells
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR14324 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184178の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
|
|
| IP |
1/20.
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). |
|
IP
1/20. |
ターゲット情報
-
関連性
TPGS1 may act in the targeting of the tubulin polyglutamylase complex. It is required for the development of the spermatid flagellum. -
細胞内局在
Microtubule. -
参照データベース
- Entrez Gene: 91978 Human
- Entrez Gene: 110012 Mouse
- Entrez Gene: 691093 Rat
- SwissProt: Q6ZTW0 Human
- SwissProt: Q99MS8 Mouse
-
別名
- C19orf20 antibody
- Gene trap ROSA b geo 22 antibody
- GTRGEO22 antibody
see all
画像
-
Western blot - Anti-TPGS1 antibody [EPR14324] (ab184178)Anti-TPGS1 antibody [EPR14324] (ab184178) at 1/10000 dilution + Mouse testis tissue lysate at 20 µg
Secondary
Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 31 kDa -
![Western blot - Anti-TPGS1 antibody [EPR14324] (ab184178)](https://www.abcam.co.jp/ps/products/184/ab184178/Images/ab184178-216527-anti-tpgs1-antibody-epr14324-western-blot.jpg)
Western blot - Anti-TPGS1 antibody [EPR14324] (ab184178)All lanes : Anti-TPGS1 antibody [EPR14324] (ab184178) at 1/2000 dilution
Lane 1 : Rat testis tissue lysate
Lane 2 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 31 kDa -
![Immunoprecipitation - Anti-TPGS1 antibody [EPR14324] (ab184178)](https://www.abcam.co.jp/ps/products/184/ab184178/Images/ab184178-216526-anti-tpgs1-antibody-epr14324-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TPGS1 antibody [EPR14324] (ab184178)Immunoprecipitation analysis of SH-SY5Y cells labeling TPGS1 using ab184178 at 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.
-
![Flow Cytometry (Intracellular) - Anti-TPGS1 antibody [EPR14324] (ab184178)](https://www.abcam.co.jp/ps/products/184/ab184178/Images/ab184178-216525-anti-tpgs1-antibody-epr14324-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-TPGS1 antibody [EPR14324] (ab184178)Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed SH-SY5Y cells staining TPGS1 using ab184178 at 1/50 and FITC conjugated Goat anti rabbit IgG at 1/150 as a secondary antibody (red curve), and a Rabbit monoclonal IgG negative control (green curve).
-
![Anti-TPGS1 antibody [EPR14324] (ab184178)](https://www.abcam.co.jp/ps/products/184/ab184178/Images/ab184178-5-benefits-of-recombinant-antibodies.png)
Anti-TPGS1 antibody [EPR14324] (ab184178)
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (2)
ab184178 は 2 報の論文で使用されています。
- Wang L et al. Regulators of tubulin polyglutamylation control nuclear shape and cilium disassembly by balancing microtubule and actin assembly. Cell Res 32:190-209 (2022). PubMed: 34782749
- Huttlin EL et al. Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 184:3022-3040.e28 (2021). PubMed: 33961781