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AB109081

Anti-TORC2 抗体 [EPR3384(2)]

Anti-TORC2 antibody [EPR3384(2)]

5

(4 Reviews)

|

(8 Publications)

Rabbit Recombinant Monoclonal TORC2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 8 publications.

別名を表示する

TORC2, CRTC2, CREB-regulated transcription coactivator 2, Transducer of regulated cAMP response element-binding protein 2, TORC-2, Transducer of CREB protein 2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TORC2 antibody [EPR3384(2)] (AB109081)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TORC2 antibody [EPR3384(2)] (AB109081)

ab109081 at 1/100 dilution staining Torc2 in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-TORC2 antibody [EPR3384(2)] (AB109081)
  • WB

AbReview40219****

Western blot - Anti-TORC2 antibody [EPR3384(2)] (AB109081)

All lanes:

Western blot - Anti-TORC2 antibody [EPR3384(2)] (ab109081) at 1/5000 dilution

All lanes:

Ins-1, rat insulinoma cell line, nuclear cell lysate at 1200000 Cells

Secondary

All lanes:

HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution

Predicted band size: 73 kDa

Observed band size: 75 kDa

true

Exposure time: 5min

This image is courtesy of an Abreview submitted by Javier Triñanes

Western blot - Anti-TORC2 antibody [EPR3384(2)] (AB109081)
  • WB

Unknown

Western blot - Anti-TORC2 antibody [EPR3384(2)] (AB109081)

All lanes:

Western blot - Anti-TORC2 antibody [EPR3384(2)] (ab109081) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

Raji cell lysate at 10 µg

Lane 3:

A673 cell lysate at 10 µg

Predicted band size: 73 kDa

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関連する標識済み抗体及び組成の異なる製品 (8)

  • Carrier free

    Anti-TORC2 antibody [EPR3384(2)] - BSA and Azide free

  • 660 APC

    APC Anti-TORC2 antibody [EPR3384(2)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-TORC2 antibody [EPR3384(2)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-TORC2 antibody [EPR3384(2)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-TORC2 antibody [EPR3384(2)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-TORC2 antibody [EPR3384(2)]

  • 578 PE

    PE Anti-TORC2 antibody [EPR3384(2)]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-TORC2 antibody [EPR3384(2)]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR3384(2)

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p>Antigen retrieval is recommended.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Stable for 12 months at -20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TORC2 also known as Target of Rapamycin Complex 2 is a multi-protein complex involved in regulating cell growth and survival. It consists of several subunits including mTOR RICTOR and mLST8 and has a molecular mass of 1-1.5 MDa. TORC2 acts as a serine/threonine kinase primarily phosphorylating Akt (Protein Kinase B) and other AGC family kinases. This complex is expressed in various tissues including liver muscle and brain where it influences multiple cellular functions.
Biological function summary

The actions of TORC2 play important roles in the regulation of lipid metabolism cytoskeletal organization and stress response. This complex contributes to cell survival and proliferation by phosphorylating specific substrates that control these processes. TORC2 specifically phosphorylates the hydrophobic motif on actor proteins like Akt which is necessary for their full activation. This activity establishes TORC2 as an important regulator within numerous cellular systems that contribute to maintaining cellular integrity and homeostasis.

Pathways

The activities of TORC2 are integral to the PI3K/Akt signaling and insulin signaling pathways. Within these pathways TORC2's phosphorylation of Akt impacts glucose metabolism protein synthesis and cell survival. It operates in parallel with TORC1 (Target of Rapamycin Complex 1) yet TORC2 responds to distinct extracellular cues highlighting its unique role. Key proteins associated with TORC2 in these pathways include PI3K upstream and Akt downstream which are essential for transmitting signals that affect cell growth and metabolic regulation.

Disruptions in TORC2 function have been associated with cancer and diabetes. The aberrant activation of TORC2 leads to unchecked cell growth and survival contributing to tumor progression in various cancer types. Additionally alterations in TORC2 activity can affect insulin signaling linking it to insulin resistance and type 2 diabetes. Proteins such as mTOR and Akt are central to these diseases with TORC2 playing a pivotal regulatory role in their pathways.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
See full target information CRTC2
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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

BMC pulmonary medicine 22:140 PubMed35410283

2022

Salt-inducible kinase 2 (SIK2) inhibitor ARN-3236 attenuates bleomycin-induced pulmonary fibrosis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Liangneng Zou,Dequn Hong,Kecong Li,Bingyuan Jiang

Bioengineered 12:10363-10372 PubMed34699314

2021

LINC00998 functions as a novel tumor suppressor in acute myeloid leukemia via regulating the ZFP36 ring finger protein/mammalian target of rapamycin complex 2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ximin Fang,Xiazhen Pan,Huirong Mai,Xiuli Yuan,Sixi Liu,Feiqiu Wen

Journal of neuroendocrinology 33:e12920 PubMed33314405

2020

Activation and expression of endogenous CREB-regulated transcription coactivators (CRTC) 1, 2 and 3 in the rat adrenal gland.

Applications

Unspecified application

Species

Unspecified reactive species

Lorna I F Smith,Zidong Zhao,Jamie Walker,Stafford Lightman,Francesca Spiga

Journal of agricultural and food chemistry 67:10513-10520 PubMed31475823

2019

CRTC2 Is a Key Mediator of Amino Acid-Induced Milk Fat Synthesis in Mammary Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Li,Chengjian Zhou,Xueying Li,Mengmeng Yu,Meng Li,Xuejun Gao

Thoracic cancer 9:136-141 PubMed29105369

2017

CRTC2 promotes non-small cell lung cancer A549 migration and invasion in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoqing Shi,Wei Luo,Rui Zhang,Chu Wang,Yuanyuan Zheng,Yunhua Song,Rongchun Wang,Liyan Zhang,Lihua Zhang,Weimin Li,Zhuang Luo

Molecular and cellular biology 37: PubMed28167604

2017

Gs-DREADD Knock-In Mice for Tissue-Specific, Temporal Stimulation of Cyclic AMP Signaling.

Applications

WB

Species

Unspecified reactive species

Dmitry Akhmedov,Maria G Mendoza-Rodriguez,Kavitha Rajendran,Mario Rossi,Jürgen Wess,Rebecca Berdeaux

Virology journal 11:30 PubMed24529027

2014

CRTC2 enhances HBV transcription and replication by inducing PGC1α expression.

Applications

WB

Species

Unspecified reactive species

Xiaohui Tian,Fei Zhao,Weihua Sun,Xiaoguang Zhi,Zhikui Cheng,Ming Zhou,Kanghong Hu

PloS one 8:e78522 PubMed24147136

2013

The short isoform of the ubiquitin ligase NEDD4L is a CREB target gene in hepatocytes.

Applications

WB

Species

Mouse

Jingqi Fu,Dmitry Akhmedov,Rebecca Berdeaux
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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