Anti-TORC2 抗体 [EPR20219] (ab184239)
![Western blot - Anti-TORC2 antibody [EPR20219] (ab184239)](https://www.abcam.co.jp/ps/products/184/ab184239/Images/ab184239-291248-anti-torc2-antibody-epr20219-western-blot.jpg "Western blot - Anti-TORC2 antibody [EPR20219] (ab184239)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20219] to TORC2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-TORC2 antibody [EPR20219]
TORC2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR20219] to TORC2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: A20, CTLL-2, YAC-1, Neuro-2a, C6, L6, A-673, Jurkat, Raji, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human fetal heart, fetal kidney and fetal brain lysates; Mouse and rat brain, kidney and spleen lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR20219 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184239の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 78 kDa (predicted molecular weight: 73 kDa).
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| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 78 kDa (predicted molecular weight: 73 kDa). |
ターゲット情報
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機能
Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). -
組織特異性
Most abundantly expressed in the thymus. Present in both B and T lymphocytes. Highly expressed in HEK293T cells and in insulinomas. High levels also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels found in brain, colon, heart, kidney, prostate, small intestine and stomach. Weak expression in liver and pancreas. -
配列類似性
Belongs to the TORC family. -
翻訳後修飾
Phosphorylation/dephosphorylation states of Ser-171 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation, is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs by LKB1. Both insulin and AMPK increase this phosphorylation, of TORC2 while glucagon suppresses it. -
細胞内局在
Cytoplasm. Nucleus. Translocated from the nucleus to the cytoplasm on interaction of the phosphorylated form with 14-3-3 protein. In response to cAMP levels and glucagon, relocated to the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 200186 Human
- Entrez Gene: 74343 Mouse
- Entrez Gene: 310615 Rat
- Omim: 608972 Human
- SwissProt: Q53ET0 Human
- SwissProt: Q3U182 Mouse
- SwissProt: Q3LRZ1 Rat
- Unigene: 406392 Human
see all -
別名
- CREB regulated transcription coactivator 2 antibody
- CREB-regulated transcription coactivator 2 antibody
- CRTC2 antibody
see all
画像
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Western blot - Anti-TORC2 antibody [EPR20219] (ab184239)All lanes : Anti-TORC2 antibody [EPR20219] (ab184239) at 1/1000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lane 3 : Human fetal brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 73 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?Exposure times: Lanes 1, 2: 3 minutes; Lane 3: 15 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-TORC2 antibody [EPR20219] (ab184239)](https://www.abcam.co.jp/ps/products/184/ab184239/Images/ab184239-291247-anti-torc2-antibody-epr20219-western-blot.jpg)
Western blot - Anti-TORC2 antibody [EPR20219] (ab184239)All lanes : Anti-TORC2 antibody [EPR20219] (ab184239) at 1/1000 dilution
Lane 1 : A20 (mouse reticulum sarcoma cell line) whole cell lysate
Lane 2 : CTLL-2 (mouse T lymphocyte cell line) whole cell lysate
Lane 3 : YAC-1 (mouse Moloney murine leukemia virus induced lymphoma lymphoblast cell line) whole cell lysate
Lane 4 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 5 : C6 (rat glial tumor cell line) whole cell lysate
Lane 6 : L6 (rat skeletal muscle cell line) whole cell lysate
Lane 7 : A-673 (human muscle Ewing's Sarcoma cell line) whole cell lysate
Lane 8 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 9 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 73 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?Exposure times: Lanes 1-3: 10 seconds; Lanes 4-9: 3 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-TORC2 antibody [EPR20219] (ab184239)](https://www.abcam.co.jp/ps/products/184/ab184239/Images/ab184239-291246-anti-torc2-antibody-epr20219-western-blot.jpg)
Western blot - Anti-TORC2 antibody [EPR20219] (ab184239)All lanes : Anti-TORC2 antibody [EPR20219] (ab184239) at 1/1000 dilution
Lane 1 : Mouse brain lysate at 10 µg
Lane 2 : Mouse kidney lysate at 10 µg
Lane 3 : Mouse spleen lysate at 10 µg
Lane 4 : Rat brain lysate at 10 µg
Lane 5 : Rat kidney lysate at 10 µg
Lane 6 : Rat spleen lysate at 10 µg
Lane 7 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg
Lane 9 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 73 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?Exposure times: Lanes 1-6: 3 minutes; Lanes 7-9: 15 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Anti-TORC2 antibody [EPR20219] (ab184239)](https://www.abcam.co.jp/ps/products/184/ab184239/Images/ab184239-4-benefits-of-recombinant-antibodies.png)
Anti-TORC2 antibody [EPR20219] (ab184239)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab184239 は論文での使用が確認できていません。