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AB109736

Anti-TORC1 抗体 [EPR3381(2)]

Anti-TORC1 antibody [EPR3381(2)]

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Rabbit Recombinant Monoclonal TORC1 antibody. Suitable for IHC-P, WB and reacts with Human samples.

別名を表示する

KIAA0616, MECT1, TORC1, WAMTP1, CRTC1, CREB-regulated transcription coactivator 1, Mucoepidermoid carcinoma translocated protein 1, Transducer of regulated cAMP response element-binding protein 1, TORC-1, Transducer of CREB protein 1

2 Images
Western blot - Anti-TORC1 antibody [EPR3381(2)] (AB109736)
  • WB

Unknown

Western blot - Anti-TORC1 antibody [EPR3381(2)] (AB109736)

All lanes:

Western blot - Anti-TORC1 antibody [EPR3381(2)] (ab109736) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Predicted band size: 67 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TORC1 antibody [EPR3381(2)] (AB109736)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TORC1 antibody [EPR3381(2)] (AB109736)

ab109736 at 1/50 dilution staining MECT1 in paraffin-embedded Human brain tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-TORC1 antibody [EPR3381(2)] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR3381(2)

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Stable for 12 months at -20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TORC1 also known as Target of Rapamycin Complex 1 is a protein complex that regulates cell growth and metabolism in response to nutrients and growth factors. This complex contains proteins such as mTOR Raptor and mLST8. The mass of mTOR the catalytic subunit is approximately 289 kDa. TORC1 is mostly expressed in tissues with high metabolic activity including the liver muscle and adipose tissue. It functions as a serine/threonine kinase playing a significant role in protein synthesis autophagy and energy metabolism.
Biological function summary

TORC1 holds a central role in cellular responses to nutrients and energy status. It is a part of the larger mechanistic target of rapamycin (mTOR) signaling pathway. This pathway influences essential cellular processes such as growth proliferation and survival. TORC1 activity increases in the presence of nutrients such as amino acids and is necessary for ribosomal biogenesis and translation initiation. It also acts as an important regulator of autophagy where its inhibition promotes autophagic degradation of cellular components balancing energy and resource availability.

Pathways

TORC1 integrates signals from related pathways such as PI3K-AKT and AMPK. These pathways facilitate the balance between anabolic and catabolic processes. The PI3K-AKT pathway stimulates TORC1 activity promoting cell growth and proliferation while the AMPK pathway inhibits TORC1 during low energy states conserving cellular resources. Proteins like TSC1/TSC2 complex and Rheb interact with TORC1 modulating its kinase activity based on cellular and environmental conditions.

TORC1 dysregulation links to conditions like cancer and type 2 diabetes. Overactivation of TORC1 contributes to unchecked cell proliferation in various cancers. Agents that inhibit TORC1 activity such as rapamycin show potential in cancer treatment to curb tumor growth. In type 2 diabetes TORC1 is connected through insulin signaling pathways as excess TORC1 activity may lead to insulin resistance. Proteins like IRS-1 which interacts with TORC1 play a role in these metabolic and proliferative disorders.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. May be required for dendritic growth of developing cortical neurons (By similarity). In concert with SIK1, regulates the light-induced entrainment of the circadian clock. In response to light stimulus, coactivates the CREB-mediated transcription of PER1 which plays an important role in the photic entrainment of the circadian clock.. (Microbial infection) Plays a role of coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
See full target information CRTC1
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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