JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB2737

Anti-TOR1AIP1 抗体 [RL13]

Anti-TOR1AIP1 antibody [RL13]

Be the first to review this product! Submit a review

|

(6 Publications)

Mouse Monoclonal TOR1AIP1 antibody. Suitable for IHC-P, ICC/IF and reacts with Rat, Mouse samples. Cited in 6 publications. Immunogen corresponding to Full Length Protein corresponding to Rat Lrrc7.

別名を表示する

Torsin-1A-interacting protein 1, Lamina-associated polypeptide 1B, LAP1B, Tor1aip1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOR1AIP1 antibody [RL13] (AB2737)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOR1AIP1 antibody [RL13] (AB2737)

Immunohistochemical analysis (Formalin/PFA-fixed paraffin-embedded sections) rat breast tissue labeling TOR1AIP1. Antigen retreived was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2737 1/50 dilution overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

Immunocytochemistry/ Immunofluorescence - Anti-TOR1AIP1 antibody [RL13] (AB2737)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TOR1AIP1 antibody [RL13] (AB2737)

Immunocytochemical analysis of NS-1 (Mouse myeloma cell line) cells labeling TOR1AIP1 with ab2737 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOR1AIP1 antibody [RL13] (AB2737)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOR1AIP1 antibody [RL13] (AB2737)

Immunohistochemical analysis (Formalin/PFA-fixed paraffin-embedded sections) of rat lymph node tissue labeling TOR1AIP1. Antigen retreived was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2737 at 1/50 dilution overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOR1AIP1 antibody [RL13] (AB2737)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOR1AIP1 antibody [RL13] (AB2737)

Immunohistochemical analysis of (Formalin/PFA-fixed paraffin-embedded sections) rat colon tissue labeling TOR1AIP1. Antigen retreived was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2737 at 1/50 dilution overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

RL13

アイソタイプ

IgG1

キャリアフリー

No

交差種

Mouse, Rat

アプリケーション

IHC-P, ICC/IF

applications

免疫原

Full Length Protein corresponding to Rat Lrrc7. The exact immunogen used to generate this antibody is proprietary information.

P70587

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製度
IgG fraction
バッファー組成
Preservative: 0.05% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TOR1AIP1 also known as lamina-associated polypeptide 1 (LAP1) is a mechanical protein involved in nuclear envelope structural integrity. This protein has an approximate mass of 75 kDa and is mainly found in the inner nuclear membrane. It interacts closely with components of the nuclear lamina particularly lamin A/C to contribute to the nuclear envelope architecture and maintain cellular functions.
Biological function summary

The protein known as LAP1 plays a role in modulating nuclear-cytoplasmic transport and chromatin dynamics. It is associated with the inner nuclear membrane complex and works in tandem with other nuclear lamins. This interaction is essential for processes like gene expression regulation and nuclear structure maintenance facilitating cellular response to mechanical stress.

Pathways

TOR1AIP1 participates in pathways involving nuclear envelope assembly and disassembly during cell division. It is integrated within the mitotic nuclear envelope reassembly pathway and interacts with proteins like emerin and torsinA. These proteins are essential for regulated nuclear envelope dynamics as well as maintaining proper chromatin organization during mitotic events.

Mutations in TOR1AIP1 have been linked to congenital muscular dystrophies and torsion dystonia. These conditions often arise from disrupted nuclear envelope integrity and abnormal cellular responses to mechanical stress. The close interaction with proteins such as emerin and torsinA underlines the connection between nuclear envelope dysfunction and the pathogenesis of these muscular and movement disorders.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Required for nuclear membrane integrity. Induces TOR1A and TOR1B ATPase activity and is required for their location on the nuclear membrane. Binds to A- and B-type lamins. Possible role in membrane attachment and assembly of the nuclear lamina.
See full target information Tor1aip1
style
left-column

文献 (6)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 282:1964-72 PubMed17121832

2006

Distinctive T cell-suppressive signals from nuclearized type 1 sphingosine 1-phosphate G protein-coupled receptors.

Applications

WB

Species

Unspecified reactive species

Jia-Jun Liao,Mei-Chuan Huang,Markus Graler,Yong Huang,Hong Qiu,Edward J Goetzl

The Journal of cell biology 139:1077-87 PubMed9382857

1998

Lamin-binding fragment of LAP2 inhibits increase in nuclear volume during the cell cycle and progression into S phase.

Applications

Unspecified application

Species

Unspecified reactive species

L Yang,T Guan,L Gerace

Molecular biology of the cell 6:1591-603 PubMed8589458

1995

Structural analysis of the p62 complex, an assembly of O-linked glycoproteins that localizes near the central gated channel of the nuclear pore complex.

Applications

Unspecified application

Species

Unspecified reactive species

T Guan,S Müller,G Klier,N Panté,J M Blevitt,M Haner,B Paschal,U Aebi,L Gerace

The Journal of biological chemistry 270:8822-8 PubMed7721789

1995

cDNA cloning and characterization of lamina-associated polypeptide 1C (LAP1C), an integral protein of the inner nuclear membrane.

Applications

Unspecified application

Species

Unspecified reactive species

L Martin,C Crimaudo,L Gerace

Cell 73:1267-79 PubMed8324822

1993

Integral membrane proteins of the nuclear envelope interact with lamins and chromosomes, and binding is modulated by mitotic phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

R Foisner,L Gerace

The Journal of cell biology 107:2029-36 PubMed3058715

1988

Integral membrane proteins specific to the inner nuclear membrane and associated with the nuclear lamina.

Applications

Unspecified application

Species

Unspecified reactive species

A Senior,L Gerace
View all publications
style
left-column
style
left-column
style
right-column

組合せ製品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB16048

Anti-Lamin B1 antibody - Nuclear Envelope Marker

BOND RX™ Validated|KO Validated|Lab Essentials

Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (AB16048)

  • 5
  • 110
Primary Antibodies

AB19319

Anti-KCNQ5 antibody

  • 0
  • 0
Primary Antibodies

AB109376

Anti-Hsp27 antibody [EPR5477]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Hsp27 antibody [EPR5477] (AB109376)

  • 5
  • 2

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com