AB12318

Anti-Topoisomerase II alpha 抗体

Name: Anti-Topoisomerase II alpha 抗体 (ab12318) | Abcam
Brand: Abcam Limited
SKU: AB12318
Rating: 3 (1 reviews)

Anti-Topoisomerase II alpha antibody

(1 Review)

(16 Publications)

Rabbit Polyclonal Topoisomerase II alpha antibody. Suitable for IHC-P, IP and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human TOP2A.

別名を表示する

TOP2, TOP2A, DNA topoisomerase 2-alpha

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody (AB12318)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody (AB12318)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human laryangeal squamous cell carcinoma labelling Topoisomerase II alpha with ab12318 at 1/1000 (1µg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-Topoisomerase II alpha antibody (AB12318)

Detection of Human Topo II Alpha by Western Blot and Immunoprecipitation.

Samples : Whole cell lysate (25 mcg/lane for A; 0.5 mg for IP in B; 25 mcg for Input in B) from HeLa cells. Antibodies : Affinity purified rabbit anti-Topo II alpha antibody BL983 used at the indicated concentrations for WB (A) and IP (B). Control antibody (Ctrl. in A) and antibody for WB of immunoprecipitated Topo II alpha was a monoclonal antibody to Topo II alpha from another source. Detection : Chemiluminescence with exposure times of 5 minutes (A) or 30 seconds (B).

All lanes:

Immunoprecipitation - Anti-Topoisomerase II alpha antibody (ab12318)

Predicted band size: 174 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IP, IHC-P

applications

免疫原

Synthetic Peptide within Human TOP2A. The exact immunogen used to generate this antibody is proprietary information.

P11388

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Purified using the peptide immobilized on solid support.

バッファー組成

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Topoisomerase II alpha also known as TOP2A is an enzyme that plays a critical role in DNA replication and cell division. It is a homodimeric protein with a mass of around 170 kDa and primarily localizes in the nucleus. TOP2A catalyzes the transient breaking and rejoining of double-stranded DNA necessary for resolving DNA tangles and supercoils during the replication process. TOP2A is highly expressed in proliferating cells due to its role in cell cycle progression making it a significant target in cancer research.

Biological function summary

TOP2A is essential for chromosome condensation segregation and relief of DNA torsional stress during mitosis. It does not function in isolation but interacts with other proteins as part of the larger DNA topoisomerase complex. This complex maintains DNA integrity during cellular processes such as transcription and replication. TOP2A's activity ensures that cells can duplicate their genetic material accurately facilitating proper cell division and maintaining genomic stability.

Pathways

TOP2A plays a significant role in the DNA replication and cell cycle regulation pathways. It interacts closely with proteins like CDC2 (cyclin-dependent kinase 1) and is regulated by cyclins which control the timing of cell cycle progression. Additionally TOP2A is involved in the DNA damage response pathway where it collaborates with proteins such as ATM (ataxia-telangiectasia mutated) emphasizing its importance in maintaining DNA structure during replication stress.

Overexpression of TOP2A is frequently associated with various cancers including breast and prostate cancer. The enzyme's increased activity promotes uncontrolled cell proliferation a hallmark of cancer development. Antitopoisomerase drugs which target this pathway are used in cancer treatments to inhibit TOP2A activity. Additionally mutations or dysregulation of TOP2A-related pathways may also contribute to chemoresistance. Understanding TOP2A interactions and regulation provides insight into its role in cancer and opens avenues for targeted therapeutic interventions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed : 17567603, PubMed : 18790802, PubMed : 22013166, PubMed : 22323612). May play a role in regulating the period length of BMAL1 transcriptional oscillation (By similarity).

See full target information TOP2A

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文献 (16)

Recent publications for all applications. Explore the full list and refine your search

Cell division 19:6 PubMed38374109

2024

Anti-cancer effects of Coix seed extract through KCTD9-mediated ubiquitination of TOP2A in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiuyang Jiang,Xue Li,Chun Zhang,Jiafu Wang,Jin Li

Life science alliance 5: PubMed36271492

2022

The human RNA polymerase I structure reveals an HMG-like docking domain specific to metazoans.

Applications

Unspecified application

Species

Unspecified reactive species

Julia L Daiß,Michael Pilsl,Kristina Straub,Andrea Bleckmann,Mona Höcherl,Florian B Heiss,Guillermo Abascal-Palacios,Ewan P Ramsay,Katarina Tlučková,Jean-Clement Mars,Torben Fürtges,Astrid Bruckmann,Till Rudack,Carrie Bernecky,Valérie Lamour,Konstantin Panov,Alessandro Vannini,Tom Moss,Christoph Engel

iScience 25:104952 PubMed36065190

2022

GINS1 promotes the proliferation and migration of glioma cells through USP15-mediated deubiquitination of TOP2A.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Yang,Xiaocen Liu,Xiaolong Zhu,Mengying Zhang,Yingying Wang,Mingzhe Ma,Kun Lv

Molecular cancer therapeutics 21:925-935 PubMed35405742

2022

YM155 Induces DNA Damage and Cell Death in Anaplastic Thyroid Cancer Cells by Inhibiting DNA Topoisomerase IIα at the ATP-Binding Site.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan P Mackay,Paul M Weinberger,John A Copland,Elahe Mahdavian,Qinqin Xu

Frontiers in cell and developmental biology 9:727836 PubMed34746125

2021

Functions of the CSB Protein at Topoisomerase 2 Inhibitors-Induced DNA Lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Franciele Faccio Busatto,Sofiane Y Mersaoui,Yilun Sun,Yves Pommier,Jean-Yves Masson,Jenifer Saffi

Frontiers in oncology 10:633142 PubMed33598437

2021

Discovery of New Catalytic Topoisomerase II Inhibitors for Anticancer Therapeutics.

Applications

Unspecified application

Species

Unspecified reactive species

Victor M Matias-Barrios,Mariia Radaeva,Yi Song,Zaccary Alperstein,Ahn R Lee,Veronika Schmitt,Joseph Lee,Fuqiang Ban,Ning Xie,Jianfei Qi,Nada Lallous,Martin E Gleave,Artem Cherkasov,Xuesen Dong

Leukemia & lymphoma 62:337-347 PubMed33131357

2020

TOP2A expression predicts responsiveness to carfilzomib in myeloma and informs novel combinatorial strategies for enhanced proteasome inhibitor cell killing.

Applications

Unspecified application

Species

Unspecified reactive species

Antonia Reale,Tiffany Khong,Sridurga Mithraprabhu,Ioanna Savvidou,Jay Hocking,Krystal Bergin,Malarmathy Ramachandran,Maoshan Chen,Francesco Dammacco,Roberto Ria,Francesco Silvestris,Angelo Vacca,John Reynolds,Andrew Spencer

Nature immunology 18:263-273 PubMed28135252

2017

The transcriptional regulator Aire binds to and activates super-enhancers.

Applications

Unspecified application

Species

Unspecified reactive species

Kushagra Bansal,Hideyuki Yoshida,Christophe Benoist,Diane Mathis

Molecular medicine reports 14:4741-4746 PubMed27748815

2016

Differential expression of multidrug resistance‑related proteins in adriamycin‑resistant (pumc‑91/ADM) and parental (pumc‑91) human bladder cancer cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Man Zhao,Shuliang Yu,Man Zhang

PloS one 10:e0142327 PubMed26560244

2015

Topoisomerase 2 Alpha Cooperates with Androgen Receptor to Contribute to Prostate Cancer Progression.

Applications

WB, ChIP

Species

Human, Human

J L Schaefer-Klein,Stephen J Murphy,Sarah H Johnson,George Vasmatzis,Irina V Kovtun

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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Anti-Topoisomerase II alpha 抗体

Anti-Topoisomerase II alpha antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody (AB12318)

Immunoprecipitation - Anti-Topoisomerase II alpha antibody (AB12318)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (16)

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