Anti-Topoisomerase I 抗体 (ab245432)
Key features and details
- Rabbit polyclonal to Topoisomerase I
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Topoisomerase I antibody
Topoisomerase I 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Topoisomerase I -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide within Human Topoisomerase I aa 715-765. The exact sequence is proprietary. NP_003277.1
Database link: P11387 -
ポジティブ･コントロール
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: Topoisomerase I IP in NIH/3T3 and HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab245432 was affinity purified using an epitope specific to Topoisomerase I immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245432の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
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| WB |
1/2000 - 1/10000.
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| 特記事項 |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
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WB
1/2000 - 1/10000. |
ターゲット情報
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機能
The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. -
関連疾患
Note=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98. -
配列類似性
Belongs to the eukaryotic type I topoisomerase family. -
翻訳後修飾
Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment. -
細胞内局在
Nucleus > nucleolus. Nucleus > nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli. - Information by UniProt
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参照データベース
- Entrez Gene: 7150 Human
- Entrez Gene: 21969 Mouse
- Omim: 126420 Human
- SwissProt: P11387 Human
- SwissProt: Q04750 Mouse
- Unigene: 472737 Human
- Unigene: 712686 Human
- Unigene: 217233 Mouse
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別名
- DNA topoisomerase 1 antibody
- DNA topoisomerase I antibody
- NUP98 fusion gene antibody
see all
画像
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Western blot - Anti-Topoisomerase I antibody (ab245432)All lanes : Anti-Topoisomerase I antibody (ab245432) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Exposure time: 30 secondsPrepared using NETN lysis buffer.
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Immunoprecipitation - Anti-Topoisomerase I antibody (ab245432)Topoisomerase I was immunoprecipitated from NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab245432 used for WB 0.1 µg/ml.
Chemiluminescence detection: 30 seconds.
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Immunoprecipitation - Anti-Topoisomerase I antibody (ab245432)Topoisomerase I was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab245432 used for IP at 6 µg per reaction. For WB 1 µg/ml.
Lane 1: ab245432 (Lot 2) IP in HeLa whole cell lysate.
Lane 2: ab245432 (Lot 3) IP in HeLa whole cell lysate.
Lane 3: Control IgG in HeLa whole cell lysate.
Chemiluminescence detection: 3 minutes.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab245432 は論文での使用が確認できていません。