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リコンビナントRabMAb

Anti-TOMM40 抗体 [EPR6932(2)] (ab185543)

Submit a review Submit a question References (8)
Western blot - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)
  • Immunoprecipitation - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)
  • Flow Cytometry (Intracellular) - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)
  • Immunocytochemistry/ Immunofluorescence - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)
  • Anti-TOMM40 antibody [EPR6932(2)] (ab185543)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6932(2)] to TOMM40
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Alkaline Phosphatase APC Carrier Free HRP PE

製品の概要

  • 製品名

    Anti-TOMM40 antibody [EPR6932(2)]
    TOMM40 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR6932(2)] to TOMM40

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HeLa and 293T cell lysate. Human liver tissue. HeLa and 293T cells

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab185543の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/300.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.
IP
1/30 - 1/50.
特記事項
Flow Cyt (Intra)
1/300.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.
IP
1/30 - 1/50.

ターゲット情報

  • 機能

    Channel-forming protein essential for import of protein precursors into mitochondria.

  • 配列類似性

    Belongs to the Tom40 family.

  • 細胞内局在

    Mitochondrion outer membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • C19orf1 antibody
      • D19S1177E antibody
      • Haymaker protein antibody
      • Mitochondrial import receptor subunit TOM40 homolog antibody
      • Mitochondrial outer membrane protein antibody
      • Mitochondrial outer membrane protein TOM40 antibody
      • p38.5 antibody
      • PER EC1 antibody
      • PEREC1 antibody
      • Probable mitochondrial import receptor subunit TOM40 homolog antibody
      • Protein Haymaker antibody
      • TOM40 antibody
      • TOM40_HUMAN antibody
      • TOMM40 antibody
      • Translocase of outer membrane 40 kDa subunit homolog antibody
      • Translocase of outer mitochondrial membrane 40 antibody
      • Translocase of outer mitochondrial membrane 40 homolog (yeast) antibody
      • Translocase of outer mitochondrial membrane 40 homolog antibody
      • Translocase of outer mitochondrial membrane 40, yeast, homolog of antibody
      see all

    画像

    • Western blot - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)
      Western blot - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)
      All lanes : Anti-TOMM40 antibody [EPR6932(2)] (ab185543) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 38 kDa
      Observed band size: 38 kDa

    • Immunoprecipitation - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)
      Immunoprecipitation - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)

      Western blot analysis of TOMM40 in immunoprecipitation pellets from HeLa lysate. ab185543 used at 1/50 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1000 dilution.

    • Flow Cytometry (Intracellular) - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)
      Flow Cytometry (Intracellular) - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)

      Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed HeLa cells labeling TOMM40 with ab185543 at 1/300 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution. 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)
      Immunocytochemistry/ Immunofluorescence - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)

      Immunofluorescent analysis of 4% paraformaldehyde fixed 293T cells labeling TOMM40 with ab185543 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TOMM40 with ab185543 at 1/500 dilution. Counter stain Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Anti-TOMM40 antibody [EPR6932(2)] (ab185543)
      Anti-TOMM40 antibody [EPR6932(2)] (ab185543)

    参考文献 (8)

    ab185543 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab185543 は 8 報の論文で使用されています。

    • Nalbach K  et al. Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2. Nat Commun 14:870 (2023). PubMed: 36797266
    • Coyne LP  et al. Mitochondrial protein import clogging as a mechanism of disease. Elife 12:N/A (2023). PubMed: 37129366
    • Yuan Q  et al. Inhibition of mitochondrial carrier homolog 2 (MTCH2) suppresses tumor invasion and enhances sensitivity to temozolomide in malignant glioma. Mol Med 27:7 (2021). PubMed: 33509092
    • Elouej S  et al. Loss of MTX2 causes mandibuloacral dysplasia and links mitochondrial dysfunction to altered nuclear morphology. Nat Commun 11:4589 (2020). PubMed: 32917887
    • Dadsena S  et al. Ceramides bind VDAC2 to trigger mitochondrial apoptosis. Nat Commun 10:1832 (2019). PubMed: 31015432
    • Li HS  et al. HIF-1a protects against oxidative stress by directly targeting mitochondria. Redox Biol N/A:101109 (2019). PubMed: 30686776
    • Kucherenko MM & Shcherbata HR Stress-dependent miR-980 regulation of Rbfox1/A2bp1 promotes ribonucleoprotein granule formation and cell survival. Nat Commun 9:312 (2018). Drosophila melanogaster . PubMed: 29358748
    • Li HS  et al. Mitochondrial targeting of HIF-1a inhibits hypoxia-induced apoptosis independently of its transcriptional activity. Free Radic Biol Med N/A:N/A (2018). PubMed: 29704620

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