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Anti-TOMM22/TOM22 抗体 [4G4] (ab57523)

Submit a review Q&A (4)References (16)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody [4G4] (ab57523)
  • Western blot - Anti-TOMM22/TOM22 antibody [4G4] (ab57523)
  • Western blot - Anti-TOMM22/TOM22 antibody [4G4] (ab57523)
  • Immunocytochemistry/ Immunofluorescence - Anti-TOMM22/TOM22 antibody [4G4] (ab57523)
  • Immunocytochemistry/ Immunofluorescence - Anti-TOMM22/TOM22 antibody [4G4] (ab57523)
  • Flow Cytometry - Anti-TOMM22/TOM22 antibody [4G4] (ab57523)

Key features and details

  • Mouse monoclonal [4G4] to TOMM22/TOM22
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-TOMM22/TOM22 antibody [4G4]
    TOMM22/TOM22 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [4G4] to TOMM22/TOM22

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Recombinant full length protein (GST-tag) corresponding to Human TOMM22/TOM22 aa 1-142.
    Sequence:

    MAAAVAAAGAGEPQSPDELLPKGDAEKPEEELEEDDDEELDETLSERLWG LTEMFPERVRSAAGATFDLSLFVAQKMYRFSRAALWIGTTSFMILVLPVV FETEKLQMEQQQQLQQRQILLGPNTGLSGGMPGALPSLPGKI


    Database link: Q9NS69
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • IHC-P: Human small Intestine. ICC/IF: HeLa cell. WB: NIH/3T3 cell lysate. Flow cyt: HeLa cells.

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab57523の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 15 kDa.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 
ICC/IF
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 15 kDa.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 
ICC/IF
Use at an assay dependent concentration.

ターゲット情報

  • 細胞内局在

    Mitochondrion outer membrane; Single-pass membrane protein

  • 参照データベース

    • 別名

      • 1C9 2 antibody
      • hTom 22 antibody
      • hTom22 antibody
      • Mitochondrial import receptor subunit TOM 22 homolog antibody
      • Mitochondrial import receptor subunit TOM22 homolog antibody
      • Mitochondrial import receptor Tom 22 antibody
      • Mitochondrial import receptor Tom22 antibody
      • MST 065 antibody
      • MST065 antibody
      • MSTP 065 antibody
      • MSTP065 antibody
      • TOM 22 antibody
      • TOM22 antibody
      • TOMM 22 antibody
      • TOMM22 antibody
      • Translocase of outer membrane 22 kDa subunit homolog antibody
      • Translocase of outer mitochondrial membrane 22 homolog antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody [4G4] (ab57523)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody [4G4] (ab57523)

      TOMM22/TOM22 antibody (ab57523) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.

      This image was generated using the ascites version of the product.

    • Western blot - Anti-TOMM22/TOM22 antibody [4G4] (ab57523)
      Western blot - Anti-TOMM22/TOM22 antibody [4G4] (ab57523)

      TOMM22/TOM22 antibody (ab57523) at 1ug/lane + NIH/3T3 cell lysate at 25ug/lane.

      This image was generated using the ascites version of the product.

    • Western blot - Anti-TOMM22/TOM22 antibody [4G4] (ab57523)
      Western blot - Anti-TOMM22/TOM22 antibody [4G4] (ab57523)
      Anti-TOMM22/TOM22 antibody [4G4] (ab57523) + immunogen peptide, Full length TOMM22 / TOM22 with GST tag.

      Predicted band size: 15 kDa



      GST tag MW is 26kDa.

    • Immunocytochemistry/ Immunofluorescence - Anti-TOMM22/TOM22 antibody [4G4] (ab57523)
      Immunocytochemistry/ Immunofluorescence - Anti-TOMM22/TOM22 antibody [4G4] (ab57523)

      Immunocytochemistry/ Immunofluorescence of TOMM22 in HeLa cell using ab57523 at 10 μg/ml.

    • Immunocytochemistry/ Immunofluorescence - Anti-TOMM22/TOM22 antibody [4G4] (ab57523)
      Immunocytochemistry/ Immunofluorescence - Anti-TOMM22/TOM22 antibody [4G4] (ab57523)

      ICC/IF image of ab57523 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57523, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      This image was generated using the ascites version of the product.

    • Flow Cytometry - Anti-TOMM22/TOM22 antibody [4G4] (ab57523)
      Flow Cytometry - Anti-TOMM22/TOM22 antibody [4G4] (ab57523)

      Overlay histogram showing HeLa cells stained with ab57523 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57523, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

      This image was generated using the ascites version of the product.

    参考文献 (16)

    ab57523 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab57523 は 16 報の論文で使用されています。

    • Zecchini V  et al. Fumarate induces vesicular release of mtDNA to drive innate immunity. Nature 615:499-506 (2023). PubMed: 36890229
    • Kim JT  et al. Regulatory Mechanism between Ferritin and Mitochondrial Reactive Oxygen Species in Spinal Ligament-Derived Cells from Ossification of Posterior Longitudinal Ligament Patient. Int J Mol Sci 24:N/A (2023). PubMed: 36769191
    • Qin X  et al. Identification of an autoinhibitory, mitophagy-inducing peptide derived from the transmembrane domain of USP30. Autophagy 18:2178-2197 (2022). PubMed: 34989313
    • Chatterjee S  et al. The type III secretion system effector EspO of enterohaemorrhagic Escherichia coli inhibits apoptosis through an interaction with HAX-1. Cell Microbiol N/A:e13366 (2021). PubMed: 34021690
    • Busch JD  et al. MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome Composition. Cell Rep 29:1728-1738.e9 (2019). PubMed: 31693908
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Question

    not getting specific band for TOMMs

    Read More
    Answer

    Hello!

    We also do recommend using RIPA buffer rather than NP40 for mitochondrial targets, would this be something you are able to do with your samples?

    Read More

    Question

    - ab57523 giving 2 prominent bands at 30kDa and 88 kDa.
    - Used TOMM22 as marker for outer mitochondrial membrane proteins
    - What is the identity of 88 kDa band?
    - protease inhibitors used
    ab48139
    - mouse cells used for mitochondrial membrane preparations
    - faint band at 88 kDa, 3 high intensity bands at 30,34 and 36 kDa

    Read More
    Answer

    Thank you very much for your patience while I was in discussion with lab.

    My colleague has confirmed that, they tested ab57523 with PC-12, Raw 264.7 and NIH/3T3 cells in western blot and they haven't observed any band at 88kDa. The band they observed was near ˜30kDa.

    For ab48139 they suggested the lower bands might be degradation product or uncharacterized proteins that cross-react with the antibody.

    I am sorry I am unable to find a more definite explanation of the bands you have been observing. We have also received no complaints for these antibodies so protocol troubleshooting might help in this case. Please try tweaking the dilution factor, lysate concentration etc.

    I hope this information will be helpful. Should you have any other question please do not hesitate to contact me.

    Read More

    Question

    It appears the protein name in Yeasts is TOM22 and not TOMM22. Below is it's DNA sequence. The currently selected gene/sequence is : TOM22/YNL131W on chromosome XIV from coordinates 378767 to 379225. This is the genomic DNA sequence and may contain untranslated regions or bases (e. g. introns, or the site of a programmed translational frameshift). For a version of the sequence that does not contain these untranslated sequence region(s), please go back and select the coding sequence option. Other Formats Available: GCG >YNL131W Chr 14 ATGGTCGAATTAACTGAAATTAAAGACGATGTCGTTCAATTAGACGAACCACAATTTTCC AGAAATCAGGCCATCGTGGAAGAAAAGGCTTCTGCAACAAACAACGACGTTGTCGATGAT GAAGATGACTCTGATAGTGATTTTGAAGATGAATTTGATGAAAATGAAACATTGTTGGAC AGAATCGTTGCTTTAAAAGACATTGTCCCCCCAGGTAAGAGACAAACAATTTCTAATTTT TTTGGTTTTACTAGCTCTTTTGTGAGAAATGCTTTCACAAAATCCGGAAACCTTGCTTGG ACTTTGACCACCACTGCTTTGTTACTCGGTGTGCCACTATCCTTATCTATACTTGCCGAA CAACAGCTAATCGAAATGGAAAAGACATTTGATTTACAAAGTGATGCTAATAACATATTG GCCCAAGGTGAAAAAGATGCTGCAGCAACAGCCAATTAA  

    Read More
    Answer

    Thank you for providing this extra information. The alignment shows a very low homology between the yeast protein and the human TOMM2 protein (www.uniprot.org/uniprot/Q9NS69), 16% only. This result suggests that unfortunately the TOMM2 antibody ab57523 is not suitable for the detection of the yeast protein. I hope this information is nevertheless useful for you. Do not hesitate to contact us again if you have other questions.

    Read More

    Question

    Dear Tech support,   What are the chances of antibodies ab57523 and ab15575 (or another sec61 antibody) of working with Yeast's proteins WB based on immunogen sequence homology? Thank you for your kind help. Regards,

    Read More
    Answer

    Thank you very much for your inquiry. Unfortunately we do not have an antibody in our catalogue for the moment for which we can suggest a crossreactivity with the yeast SEC61 proteins. I am very sorry for this. Please see further below for the alignment scores. For the TOMM22 protein, I could not find the sequence in UniProt neither. Can the customer maybe provide this? In the case we can not make an alignment, I am willing to provide a testing discount. alignment scores for SEC61 antibodies: ab15575 44% yeast/ 61% schp http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20110905-173253-0036-74194366-pg ab77789 and ab15037 14% yeast/schp http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20110905-173532-0061-46398952-pg ab97537 15% yeast/schp http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20110905-173733-0295-24017780-pg ab1327 14% yeast/schp http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20110905-173823-0394-68897710-pg Unfortunately I have not found the SEC61B and G yeast sequences on UniProt and can therefore not provide an alignment. Do you have these sequences? I am sorry for not being able to be more helpful on this occasion. Please provide me with the sequences if you can in order for me to do the alignments. Thank you very much.

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