Name: Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker
Brand: RabMAb
SKU: ab186734
Rating: 3 (9 reviews)

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Western blot - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15581-39] to TOMM20 - Mitochondrial Marker
Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WB
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 647 Carrier Free HRP

製品名

Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker
TOMM20 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR15581-39] to TOMM20 - Mitochondrial Marker
由来種

Rabbit
アプリケーション

適用あり: Flow Cyt (Intra), IHC-P, ICC/IF, WBmore details
種交差性

交差種: Mouse, Rat, Human
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: HepG2 and HeLa whole cell lysate (ab150035). IHC-P: Human endometrial adenocarcinoma, human cervical cancer, mouse cardiac muscle tissues, rat testis, mouse colon. ICC/IF: HeLa cells. Flow Cyt (intra): NCI-H2228 cells.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR15581-39
アイソタイプ

IgG
研究分野
- Neuroscience
- Processes

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab186734の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
Flow Cyt (Intra)		1/40. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P	(1)	1/50 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF		1/250 - 1/500.
WB	(5)	1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).

特記事項
Flow Cyt (Intra) 1/40. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
WB 1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).

機能

Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.
配列類似性

Belongs to the Tom20 family.
細胞内局在

Mitochondrion outer membrane.
Target information above from: UniProt accession Q15388 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 9804 Human
- Entrez Gene: 67952 Mouse
- Entrez Gene: 266601 Rat
- Omim: 601848 Human
- SwissProt: Q15388 Human
- SwissProt: Q9DCC8 Mouse
- SwissProt: Q62760 Rat
- Unigene: 533192 Human
- Unigene: 380026 Mouse
- Unigene: 6932 Mouse
- Unigene: 2143 Rat
see all
別名
- KIAA0016 antibody
- MAS20 antibody
- MGC117367 antibody
- Mitochondrial 20 kDa outer membrane protein antibody
- Mitochondrial import receptor subunit TOM20 homolog antibody
- MOM19 antibody
- Outer mitochondrial membrane receptor Tom20 antibody
- TOM20 antibody
- TOM20_HUMAN antibody
- TOMM20 antibody
- Translocase of outer mitochondrial membrane 20 homolog (yeast) antibody
- Translocase of outer mitochondrial membrane 20 homolog type II antibody
see all

Western blot - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)

Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734) at 1/5000 dilution + HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 16 kDa
Observed band size: 16 kDa
Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)

Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cells) labeling TOMM20 with ab186734 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/100 was used as counterstain antibody.

Confocal image showing cytoplasmic staining in HeLa cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue, labeling TOMM20 with ab186734 at 1/1000 dilution. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).

Granular cytoplasmic staining on human cervical cancer.
Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)

Intracellular Flow Cytometry analysis of TOMM20 expression in NCI-H2228 (Human lung cancer cell line) cells using ab186734 at 1/40 dilution (pink) and a rabbit IgG as negative control (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue, labeling TOMM20 with ab186734 at 1/1000 dilution. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).

Granular cytoplasmic staining on mouse colon.
Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)Samandi et al eLife. 2017; 6: e27860. Published online 2017 Oct 30. doi: 10.7554/eLife.27860

AltMiD51^5’ expression induces mitochondrial fission.

Confocal microscopy of mock-transfected cells, cells transfected with altMiD51^WT, altMiD51^LYR→AAA or Drp1^K38A immunostained with anti-TOM20 (red channel) and anti-Flag (green channel) monoclonal antibodies. In each image, boxed areas are shown at higher magnification in the bottom right corner.

TOMM20 is detected using ab186734 at 1/500 dilution.

From Figure 12c of Samandi et al.

Samandi et al eLife. 2017; 6: e27860. Published online 2017 Oct 30. doi: 10.7554/eLife.27860

Reproduced underthe Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, labeling TOMM20 (green) with ab186734 at 1/250 dilution.

Alexa Fluor^®488-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)

Immunohistochemical analysis of paraffin-embedded rat testis tissue, labeling TOMM20 with ab186734 at 1/1000 dilution. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).

Granular cytoplasmic staining on rat testis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)

Immunohistochemical analysis of paraffin-embedded human endometrial adenocarcinoma tissue, labeling TOMM20 with ab186734 at 1/100 dilution.

Detected using HRP polymer for Rabbit IgG and counterstained using hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling TOMM20 with ab186734 at 1/100 dilution.

Detected using HRP polymer for Rabbit IgG and counterstained using hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)

Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734) at 1/20000 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 16 kDa
Observed band size: 16 kDa
Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab186734 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab186734 は 93 報の論文で使用されています。

van Beek SMM et al. Effect of β2-agonist treatment on insulin-stimulated peripheral glucose disposal in healthy men in a randomised placebo-controlled trial. Nat Commun 14:173 (2023). PubMed: 36635304
Adriaenssens E et al. Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space. Nat Cell Biol 25:467-480 (2023). PubMed: 36690850
Gemmink A et al. Twenty-four hour rhythmicity in mitochondrial network connectivity and mitochondrial respiration; a study in human skeletal muscle biopsies of young lean and older individuals with obesity. Mol Metab 72:101727 (2023). PubMed: 37062525
Bodenstein DF et al. Optimization of differential filtration-based mitochondrial isolation for mitochondrial transplant to cerebral organoids. Stem Cell Res Ther 14:202 (2023). PubMed: 37580812
Underwood EL et al. Enhanced presynaptic mitochondrial energy production is required for memory formation. Sci Rep 13:14431 (2023). PubMed: 37660191

View all Publications for this product

レビューと Q&A

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レビューを送る質問を送る

1-9 of 9 Abreviews or Q&A

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (brain)

Permeabilization

Yes - CAS block

Specification

brain

Blocking step

CAS block as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 12 2018

Application

Immunocytochemistry

Sample

Rat Cultured Cells (Cardiomyocytes)

Permeabilization

Yes - Triton x-100, 0.03%

Specification

Cardiomyocytes

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 21 2018

Application

Immunohistochemistry (Frozen sections)

Sample

Rat Tissue sections (Heart)

Permeabilization

Yes - Triton x-100, 0.03%

Specification

Heart

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 13 2017

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Heart)

Antigen retrieval step

None

Permeabilization

Yes - Triton x-100, 0.03%

Specification

Heart

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 13 2017

Application

Western blot

Sample

Zebrafish Tissue lysate - whole (Retina)

Gel Running Conditions

Reduced Denaturing (15% gel)

Loading amount

20 µg

Specification

Retina

Blocking step

Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 13 2017

Application

Western blot

Sample

Mouse Tissue lysate - whole (Liver, retina)

Gel Running Conditions

Reduced Denaturing (15% gel)

Loading amount

20 µg

Specification

Liver, retina

Blocking step

Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 13 2017

Application

Western blot

Sample

Human Cell lysate - whole cell (HEK)

Gel Running Conditions

Reduced Denaturing (15% gel)

Loading amount

20 µg

Specification

HEK

Blocking step

Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 13 2017

Application

Western blot

Sample

Human Cell lysate - whole cell (Heart)

Gel Running Conditions

Reduced Denaturing (15%)

Loading amount

25 µg

Specification

Heart

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 21 2016

Application

Western blot

Sample

Mouse Cell lysate - whole cell (Heart)

Gel Running Conditions

Reduced Denaturing (15%)

Loading amount

25 µg

Specification

Heart

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 21 2016

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

Related Products

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

Certificate of Compliance

参考文献 (93)

レビューと Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker

Immunocytochemistry abreview for Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker

Immunohistochemistry (Frozen sections) abreview for Anti-TOMM20 antibody [EPR15581] - Mitochondrial Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TOMM20 antibody [EPR15581] - Mitochondrial Marker

Western blot abreview for Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker

Western blot abreview for Anti-TOMM20 antibody [EPR15581] - Mitochondrial Marker

Western blot abreview for Anti-TOMM20 antibody [EPR15581] - Mitochondrial Marker

Western blot abreview for Anti-TOMM20 antibody [EPR15581] - Mitochondrial Marker

Western blot abreview for Anti-TOMM20 antibody [EPR15581] - Mitochondrial Marker