Anti-TOM70 抗体 [EPR26576-162] - BSA and Azide free (ab290002)

製品名

Anti-TOM70 antibody [EPR26576-162] - BSA and Azide free
TOM70 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR26576-162] to TOM70 - BSA and Azide free
由来種

Rabbit
特異性

This antibody does not react with Mouse and Rat species for IHC
アプリケーション

適用あり: ICC/IF, Flow Cyt (Intra), WB, IHC-P, IPmore details
種交差性

交差種: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: NIH/3T3, C6, HepG2, HeLa and U-2 OS whole cell lysates; Mouse brain and liver tissue lysates; Rat brain, liver and breast tissue lysates. IHC-P: Human colon, esophagus, breast cancer and cerebrum tissues. ICC/IF: U-2 OS, NIH/3T3 and C6 cells. Flow Cyt: U-2 OS, NIH/3T3 and C6 cells. IP: C6, NIH/3T3 and HeLa whole cell lysates.
特記事項
ab290002 is the carrier-free version of ab289977.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C.
バッファー

pH: 7.20
Constituent: 100% PBS
キャリア・フリー

はい
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR26576-162
アイソタイプ

IgG
研究分野

Compatible Secondaries
KO cell lines
- Human TOMM70A (TOM70) knockout HeLa cell line (ab265396)
KO cell lysates
- Human TOMM70A (TOM70) knockout HeLa cell lysate (ab258732)
Related Products
- Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab290002の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
ICC/IF		Use at an assay dependent concentration.
Flow Cyt (Intra)		Use at an assay dependent concentration.
WB		Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 67 kDa).
IHC-P		Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Only suitable for Human.
IP		Use at an assay dependent concentration.

特記事項
ICC/IF Use at an assay dependent concentration.
Flow Cyt (Intra) Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 67 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Only suitable for Human.
IP Use at an assay dependent concentration.

機能

Receptor that accelerates the import of all mitochondrial precursor proteins.
配列類似性

Belongs to the Tom70 family.
Contains 10 TPR repeats.
細胞内局在

Mitochondrion outer membrane.
Target information above from: UniProt accession O94826 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 9868 Human
- Entrez Gene: 28185 Mouse
- Entrez Gene: 304017 Rat
- Omim: 606081 Human
- SwissProt: O94826 Human
- SwissProt: Q9CZW5 Mouse
- SwissProt: Q75Q39 Rat
- Unigene: 227253 Human
- Unigene: 213292 Mouse
- Unigene: 61703 Rat
see all
別名
- FLJ9047 antibody
- Mitochondrial import receptor subunit TOM70 antibody
- Mitochondrial precursor proteins import receptor antibody
- TOM70 antibody
- TOM70_HUMAN antibody
- Tomm70a antibody
- Translocase of outer membrane 70 kDa subunit antibody
- Translocase of outer membrane TOM70 antibody
- Translocase of outer mitochondrial membrane 70 homolog A antibody
see all

Western blot - Anti-TOM70 antibody [EPR26576-162] - BSA and Azide free (ab290002)

All lanes : Anti-TOM70 antibody [EPR26576-162] (ab289977) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TOM70 knockout HeLa cell lysate
Lane 3 : U-2 OS cell lysate
Lane 4 : Mouse Brain cell lysate
Lane 5 : Rat Brain cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Performed under reducing conditions.

Predicted band size: 67 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?

False colour image of Western blot: Anti-TOM70 antibody [EPR26576-162] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab289977 was shown to bind specifically to TOM70. A band was observed at 72 kDa in wild-type HeLa cell lysates with no signal observed at this size in TOMM70 knockout cell line ab265396 (knockout cell lysate ab258732). To generate this image, wild-type and TOMM70 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

This data was developed using ab289977, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOM70 antibody [EPR26576-162] - BSA and Azide free (ab290002)

This data was developed using ab289977, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling TOM70 with ab289977 at 1/2000 dilution followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebrum. The section was incubated with ab289977 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins.
Flow Cytometry (Intracellular) - Anti-TOM70 antibody [EPR26576-162] - BSA and Azide free (ab290002)

This data was developed using ab289977, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized C6 (rat glial tumor glial cell) cells labelling TOM70 with ab289977 at 1/500 dilution (0.1µg) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-TOM70 antibody [EPR26576-162] - BSA and Azide free (ab290002)

This data was developed using ab289977, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 cells labeling TOM70 with ab289977 at 1/100 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in C6 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution.
Immunoprecipitation - Anti-TOM70 antibody [EPR26576-162] - BSA and Azide free (ab290002)

This data was developed using ab289977, the same antibody clone in a different buffer formulation.

TOM70 was immunoprecipitated from C6 (rat glial tumor glial cell) whole cell lysate with ab289977 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289977 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: C6 (rat glial tumor glial cell) whole cell lysate 10 µg

Lane 2: ab289977 IP in C6 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab289977 in C6 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3.25 seconds
Western blot - Anti-TOM70 antibody [EPR26576-162] - BSA and Azide free (ab290002)

All lanes : Anti-TOM70 antibody [EPR26576-162] (ab289977) at 1/1000 dilution

Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : Mouse breast tissue lysate
Lane 5 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 6 : Rat brain tissue lysate
Lane 7 : Rat liver tissue lysate
Lane 8 : Rat breast tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 67 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?

This data was developed using 289977, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 32356556)

Low expression: Breast (human protein atlas: https://www.proteinatlas.org/ENSG00000154174-TOMM70)

Exposure time: 15 seconds
Flow Cytometry (Intracellular) - Anti-TOM70 antibody [EPR26576-162] - BSA and Azide free (ab290002)

This data was developed using ab289977, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol, permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling TOM70 with ab289977 at 1/500 dilution (0.1µg) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-TOM70 antibody [EPR26576-162] - BSA and Azide free (ab290002)

This data was developed using ab289977, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labeling TOM70 with ab289977 at 1/100 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing cytoplasmic staining in NIH/3T3 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution.
Flow Cytometry (Intracellular) - Anti-TOM70 antibody [EPR26576-162] - BSA and Azide free (ab290002)

This data was developed using ab289977, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol, permeabilized U-2 OS (human bone osteosarcoma epithelial cell) cells labelling TOM70 with ab289977 at 1/500 dilution (0.1µg) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOM70 antibody [EPR26576-162] - BSA and Azide free (ab290002)

This data was developed using ab289977, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labelling TOM70 with ab289977 at 1/2000 dilution followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human breast cancer. The section was incubated with ab289977 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins
Immunocytochemistry/ Immunofluorescence - Anti-TOM70 antibody [EPR26576-162] - BSA and Azide free (ab290002)

This data was developed using ab289977, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS cells labeling TOM70 with ab289977 at 1/100 dilution, followed by ab150081 Goat Anti-Rbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing mitochondrial staining in U-2 OS cell line. ab33985 Anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution.
Western blot - Anti-TOM70 antibody [EPR26576-162] - BSA and Azide free (ab290002)

All lanes : Anti-TOM70 antibody [EPR26576-162] (ab289977) at 1/1000 dilution

Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 67 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?

This data was developed using 289977, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 31907385, PMID:11956321).

Exposure time: 3.25 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOM70 antibody [EPR26576-162] - BSA and Azide free (ab290002)

This data was developed using ab289977, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human esophagus tissue labelling TOM70 with ab289977 at 1/2000 dilution followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human esophagus. The section was incubated with ab289977 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins
Immunoprecipitation - Anti-TOM70 antibody [EPR26576-162] - BSA and Azide free (ab290002)

This data was developed using ab289977, the same antibody clone in a different buffer formµlation.
TOM70 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg with ab290002 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab290002 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg

Lane 2: ab289977 IP in HeLa whole cell lysate

Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab289977 in HeLa whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3.25 seconds
Immunoprecipitation - Anti-TOM70 antibody [EPR26576-162] - BSA and Azide free (ab290002)

This data was developed using ab289977, the same antibody clone in a different buffer formulation.

TOM70 was immunoprecipitated from NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab289977 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289977 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 µg

Lane 2: ab289977 IP in NIH/3T3 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab289977 in NIH/3T3 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3.25 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOM70 antibody [EPR26576-162] - BSA and Azide free (ab290002)

This data was developed using ab289977, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling TOM70 with ab289977 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human colon. The section was incubated with ab289977 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins
Anti-TOM70 antibody [EPR26576-162] - BSA and Azide free (ab290002)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet download

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製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

キャリア・フリー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Compatible Secondaries

KO cell lines

KO cell lysates

Related Products

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

Datasheet download

Certificate of Compliance

参考文献 (0)

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