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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-TOM1 抗体 [EPR11989(B)] (ab170928)

Submit a review Submit a question References (1)
Flow Cytometry (Intracellular) - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
  • Western blot - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
  • Western blot - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
  • Anti-TOM1 antibody [EPR11989(B)] (ab170928)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11989(B)] to TOM1
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-TOM1 antibody [EPR11989(B)]
    TOM1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR11989(B)] to TOM1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-Pmore details
    適用なし: ICC/IF or IP

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HepG2, HeLa, K562 and JAR cell lysates; Human liver and stomach tissues; K562 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR11989(B)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab170928の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Predicted molecular weight: 54 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Predicted molecular weight: 54 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
追加情報
Is unsuitable for ICC/IF or IP.

ターゲット情報

  • 関連性

    Function: May be involved in intracellular trafficking. Probable association with membranes. Tissue specificity: Widely expressed. Highly expressed in skeletal muscle, heart, placenta and liver. Similarity: Belongs to the TOM1 family. Contains 1 GAT domain. Contains 1 VHS domain.

  • 細胞内局在

    Cytoplasm. Membrane; Peripheral membrane protein

  • 参照データベース

    see all

  • 別名

    • FLJ33404 antibody
    • OTTHUMP00000028777 antibody
    • Target of myb 1 antibody
    • Target of Myb protein 1 antibody
    • Target of myb1 antibody
    • TOM 1 antibody
    • TOM1 antibody
    • TOM1_HUMAN antibody
    see all

画像

  • Flow Cytometry (Intracellular) - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
    Flow Cytometry (Intracellular) - Anti-TOM1 antibody [EPR11989(B)] (ab170928)

    Intracellular flow cytometric analysis of permeabilized K562 cellslabeling TOM1 with ab170928 at 1/100 dilution (red) compared toa rabbit IgG negative control (green). 

     

     

  • Western blot - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
    Western blot - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
    All lanes : Anti-TOM1 antibody [EPR11989(B)] (ab170928) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : TOM1 knockout HAP1 whole cell lysate
    Lane 3 : HepG2 whole cell lysate
    Lane 4 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 54 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab170928 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab170928 was shown to specifically react with in wild-type HAP1 cells as signal was lost in TOM1 knockout cells. Wild-type and TOM1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab170928 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOM1 antibody [EPR11989(B)] (ab170928)

    Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling TOM1 with ab170928 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOM1 antibody [EPR11989(B)] (ab170928)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TOM1 with ab170928 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
    Western blot - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
    All lanes : Anti-TOM1 antibody [EPR11989(B)] (ab170928) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : JAR cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 54 kDa

  • Anti-TOM1 antibody [EPR11989(B)] (ab170928)
    Anti-TOM1 antibody [EPR11989(B)] (ab170928)

参考文献 (1)

ab170928 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab170928 は 1 報の論文で使用されています。

  • Lobingier BT  et al. An Approach to Spatiotemporally Resolve Protein Interaction Networks in Living Cells. Cell 169:350-360.e12 (2017). PubMed: 28388416

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