Anti-Tollip 抗体 (ab187198)
Key features and details
- Rabbit polyclonal to Tollip
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Tollip antibody
Tollip 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Tollip -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken, Cow
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免疫原
Recombinant full length protein corresponding to Human Tollip aa 1 to the C-terminus.
Database link: Q9H0E2 -
ポジティブ･コントロール
- WB: SW480, OVCAR3, MCF-7 and 293T cell lysates, mouse spleen, intestine and liver tissue lysates; IHC: Rat brain; ICC/IF: L929 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187198の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/50 - 1/200.
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| WB |
1/500 - 1/2000. Predicted molecular weight: 30 kDa.
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| IHC-P |
1/50 - 1/200.
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| 特記事項 |
|---|
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ICC/IF
1/50 - 1/200. |
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WB
1/500 - 1/2000. Predicted molecular weight: 30 kDa. |
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IHC-P
1/50 - 1/200. |
ターゲット情報
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機能
Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity. -
配列類似性
Belongs to the tollip family.
Contains 1 C2 domain.
Contains 1 CUE domain. -
翻訳後修飾
Phosphorylated by IRAK1 upon stimulation by IL-1 or microbial products. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 423099 Chicken
- Entrez Gene: 539480 Cow
- Entrez Gene: 54472 Human
- Entrez Gene: 54473 Mouse
- Entrez Gene: 361677 Rat
- Omim: 606277 Human
- SwissProt: Q5ZK05 Chicken
- SwissProt: Q2LGB5 Cow
see all -
別名
- Adapter protein antibody
- FLJ33531 antibody
- IL 1RAcPIP antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tollip antibody (ab187198)
Paraffin-embedded rat brain tissue stained for Tollip using ab187198 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Tollip antibody (ab187198)Immunofluorescence staining of L929 cells stained for Tollip with ab187198 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
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Western blot - Anti-Tollip antibody (ab187198)All lanes : Anti-Tollip antibody (ab187198) at 1/1000 dilution
Lane 1 : SW480 cell lysate
Lane 2 : OVCAR3 cell lysate
Lane 3 : MCF-7 cell lysate
Lane 4 : 293T cell lysate
Lane 5 : Mouse spleen tissue lysate
Lane 6 : Mouse intestine tissue lysate
Lane 7 : Mouse liver tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Predicted band size: 30 kDaBlocking buffer: 3% nonfat dry milk in TBST.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab187198 は 9 報の論文で使用されています。
- Hao J et al. NLRC5 restricts dengue virus infection by promoting the autophagic degradation of viral NS3 through E3 ligase CUL2 (cullin 2). Autophagy 19:1332-1347 (2023). PubMed: 36126167
- Nalbach K et al. Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2. Nat Commun 14:870 (2023). PubMed: 36797266
- Siebert A et al. ALS-linked loss of Cyclin-F function affects HSP90. Life Sci Alliance 5:N/A (2022). PubMed: 36114006
- Holani R et al. Cathelicidins Induce Toll-Interacting Protein Synthesis to Prevent Apoptosis in Colonic Epithelium. J Innate Immun N/A:1-18 (2022). PubMed: 36116427
- Wu JG et al. MiRNA-6870-3p Regulates Lipopolysaccharide Induced Epicardial Adipose Tissue Inflammatory Genes via Targeting Tollip-Mediated JNK and NF-κB Signaling in Coronary Artery Disease. Int Heart J 63:915-927 (2022). PubMed: 36184551
- Pokatayev V et al. Homeostatic regulation of STING protein at the resting state by stabilizer TOLLIP. Nat Immunol 21:158-167 (2020). PubMed: 31932809
- Lin CY et al. Autophagy Receptor Tollip Facilitates Bacterial Autophagy by Recruiting Galectin-7 in Response to Group A Streptococcus Infection. Front Cell Infect Microbiol 10:583137 (2020). PubMed: 33425778
- Humbert-Claude M et al. Tollip, an early regulator of the acute inflammatory response in the substantia nigra. J Neuroinflammation 13:303 (2016). PubMed: 27927222
- Fernandes P et al. Differential expression of key regulators of Toll-like receptors in ulcerative colitis and Crohn's disease: a role for Tollip and peroxisome proliferator-activated receptor gamma? Clin Exp Immunol 183:358-68 (2016). PubMed: 26462859