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Anti-TNNT3 抗体 - C-terminal (ab175058)

Reviews (1) Submit a question References (1)
Western blot - Anti-TNNT3 antibody - C-terminal (ab175058)

    Key features and details

    • Goat polyclonal to TNNT3 - C-terminal
    • Suitable for: IHC-P, WB
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-TNNT3 antibody - C-terminal
      TNNT3 一次抗体 製品一覧

    • 製品の詳細

      Goat polyclonal to TNNT3 - C-terminal

    • 由来種

      Goat

    • 特異性

      ab175058 is expected to recognize isoform 1, 2 and 4 (NP_006748.1; NP_001036246.1; NP_001036247.1).

    • アプリケーション

      適用あり: IHC-P, WBmore details

    • 種交差性

      交差種: Mouse, Rat, Human

    • 免疫原

      Synthetic peptide within Human TNNT3 aa 240-254 (C terminal) (Cysteine residue). The exact sequence is proprietary. NP_006748.1; NP_001036246.1; NP_001036247.1
      Sequence:

      TTLRSRIDQAQKHSK


      Database link: P45378
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • ポジティブ･コントロール

      • Human, Mouse and Rat skeletal muscle lysates

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    • バッファー

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 0.5% BSA, 99% Tris buffered saline
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • 特記事項（精製）

      ab175058 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab175058の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P (1)
    Use a concentration of 4 - 8 µg/ml.
    WB
    Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 32 kDa.

    1 hour primary incubation is recommended for this product.
    特記事項
    IHC-P
    Use a concentration of 4 - 8 µg/ml.
    WB
    Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 32 kDa.

    1 hour primary incubation is recommended for this product.

    ターゲット情報

    • 機能

      Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

    • 組織特異性

      In fetal and adult fast skeletal muscles, with a higher level expression in fetal than in adult muscle.

    • 関連疾患

      Defects in TNNT3 are a cause of distal arthrogryposis type 2B (DA2B) [MIM:601680]; also known as arthrogryposis multiplex congenita, distal, type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin.

    • 配列類似性

      Belongs to the troponin T family.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • AMCD2B antibody
      • Beta TnTF antibody
      • Beta-TnTF antibody
      • DA2B antibody
      • DKFZp779M2348 antibody
      • fast skeletal muscle antibody
      • Fast skeletal muscle troponin T antibody
      • FSSV antibody
      • fTnT antibody
      • TNNT 3 antibody
      • Tnnt3 antibody
      • TNNT3_HUMAN antibody
      • TnTf antibody
      • Troponin T antibody
      • Troponin T fast skeletal muscle antibody
      • Troponin T type 3 (skeletal fast) antibody
      • Troponin T type 3 antibody
      • Troponin T3 skeletal fast antibody
      see all

    画像

    • Western blot - Anti-TNNT3 antibody - C-terminal (ab175058)
      Western blot - Anti-TNNT3 antibody - C-terminal (ab175058)
      All lanes : Anti-TNNT3 antibody - C-terminal (ab175058) at 0.01 µg/ml

      Lane 1 : Human skeletal muscle lysate
      Lane 2 : Mouse skeletal muscle lysate
      Lane 3 : Rat skeletal muscle lysate

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 32 kDa

    参考文献 (1)

    ab175058 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab175058 は 1 報の論文で使用されています。

    • Lu J  et al. The distal arthrogryposis-linked p.R63C variant promotes the stability and nuclear accumulation of TNNT3. J Clin Lab Anal 35:e24089 (2021). PubMed: 34766372

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TNNT3 antibody - C-terminal

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Skeletal muscles of thigh)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM Tris/1 mM EDTA (pH9.0)
    Permeabilization
    No
    Specification
    Skeletal muscles of thigh
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    投稿 Jul 27 2023

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