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AB323371

Anti-TNFRSF10B 抗体 [RM2078]

Anti-TNFRSF10B antibody [RM2078]

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Rabbit Recombinant Multiclonal DR5 antibody. Suitable for WB and reacts with Human, Mouse samples.

別名を表示する

CD262, Dr5, Killer, Tnfrsf10b, Tumor necrosis factor receptor superfamily member 10B, Death receptor 5, MK

2 Images
Western blot - Anti-TNFRSF10B antibody [RM2078] (AB323371)
  • WB

Supplier Data

Western blot - Anti-TNFRSF10B antibody [RM2078] (AB323371)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, ab323371 was shown to bind specifically to TNFRSF10B. Target of interest was observed at 40, 48 kDa in wild-type HeLa cell lysates (lane 1) with no signal observed at this size in TNFRSF10B knockout cell line (lane 2) (lane 2, knockout cell line ab264922).

The lanes 1-5 were developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

Doxorubicin treatment elevated the expression of DR5 (PMID : 12496481; PMID : 11468181; PMID : 11090076).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane1-4 : 103 seconds; lane 5-6 : 59 seconds

All lanes:

Western blot - Anti-TNFRSF10B antibody [RM2078] (ab323371) at 1/1000 dilution

Lane 1:

Parental HeLa (human cervical adenocarcinoma epithelial cell) whole lysate at 20 µg

Lane 2:

TNFRSF10B knockout HeLa whole lysate at 20 µg

Lane 3:

HT-1080 (human fibrosarcoma epithelial cell) whole lysate at 20 µg

Lane 4:

HepG2 (human hepatocelullar carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

Untreated HCT 116 (human colorectal carcinoma epithelial cell) whole lysate at 20 µg

Lane 6:

HCT 116 treated with 0.5uM doxorubicin for 24 hours whole lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 40 kDa,48 kDa,36 kDa

true

Western blot - Anti-TNFRSF10B antibody [RM2078] (AB323371)
  • WB

Supplier Data

Western blot - Anti-TNFRSF10B antibody [RM2078] (AB323371)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Neuro-2a

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-TNFRSF10B antibody [RM2078] (ab323371) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

NIH/3T3 transfected with siRNA specifically targeting DR5 whole cell lysate at 20 µg

Lane 3:

C2C12 (mouse myoblast) whole cell lysate at 20 µg

Lane 4:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 5:

MEF (mouse embryo fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 48 kDa,36 kDa

false

Exposure time: 59s

Key facts

宿主種

Rabbit

クローン性

Multiclonal

クローン番号

RM2078

アイソタイプ

IgG

キャリアフリー

No

交差種

Human, Mouse

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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組合せ製品

Assay Kits

AB46013

Human DR5 ELISA Kit (CD262)

Sandwich ELISA - Human DR5 ELISA Kit (CD262) (AB46013)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis.
See full target information Tnfrsf10b
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Abcam product promise

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