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Anti-TNF alpha 抗体 [V1q(H8)] (ab8097)

Submit a review Q&A (1)References (4)

Key features and details

  • Rat monoclonal [V1q(H8)] to TNF alpha
  • Suitable for: Inhibition Assay, Neutralising, Flow Cyt
  • Reacts with: Mouse
  • Isotype: IgD

製品の概要

  • 製品名

    Anti-TNF alpha antibody [V1q(H8)]
    TNF alpha 一次抗体 製品一覧

  • 製品の詳細

    Rat monoclonal [V1q(H8)] to TNF alpha

  • 由来種

    Rat

  • 特異性

    Anti-mouse TNF-a reacts with both natural and recombinant TNF-a and shows neutralizing activity.

  • アプリケーション

    適用あり: Inhibition Assay, Neutralising, Flow Cytmore details

  • 種交差性

    交差種: Mouse

  • 免疫原

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Monocytes/macrophages

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab8097の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Inhibition Assay
Use at an assay dependent concentration.
Neutralising
Use at an assay dependent concentration. PubMed: 23300453

The antibody was functionally tested by neutralization of the cytopathic effect of cytotoxin in the L929 TNF bioassay. The biological activity of the antibody can be defined as the concentration of V1q required to neutralize 100 U/ml of TNF/cytotoxin.
Flow Cyt
Use at an assay dependent concentration.

Stains the extracellular domain. The CHO cells were fixed in PBA containing 0.2 % formaldehyde before staining.
特記事項
Inhibition Assay
Use at an assay dependent concentration.
Neutralising
Use at an assay dependent concentration. PubMed: 23300453

The antibody was functionally tested by neutralization of the cytopathic effect of cytotoxin in the L929 TNF bioassay. The biological activity of the antibody can be defined as the concentration of V1q required to neutralize 100 U/ml of TNF/cytotoxin.
Flow Cyt
Use at an assay dependent concentration.

Stains the extracellular domain. The CHO cells were fixed in PBA containing 0.2 % formaldehyde before staining.

ターゲット情報

  • 機能

    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

  • 関連疾患

    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).

  • 配列類似性

    Belongs to the tumor necrosis factor family.

  • 翻訳後修飾

    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.

  • 細胞内局在

    Secreted and Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • APC1 antibody
      • APC1 protein antibody
      • Cachectin antibody
      • DIF antibody
      • Differentiation inducing factor antibody
      • Macrophage cytotoxic factor antibody
      • Tnf antibody
      • TNF superfamily member 2 antibody
      • TNF superfamily, member 2 antibody
      • TNF, macrophage derived antibody
      • TNF, monocyte derived antibody
      • TNF-a antibody
      • TNF-alpha antibody
      • TNFA antibody
      • TNFA_HUMAN antibody
      • TNFSF2 antibody
      • Tumor necrosis factor (TNF superfamily member 2) antibody
      • Tumor necrosis factor alpha antibody
      • Tumor necrosis factor antibody
      • Tumor necrosis factor ligand superfamily member 2 antibody
      • Tumor Necrosis Factor, Membrane Form antibody
      • Tumor necrosis factor, soluble form antibody
      see all

    参考文献 (4)

    ab8097 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab8097 は 4 報の論文で使用されています。

    • Helk E  et al. TNFa-mediated liver destruction by kupffer cells and Ly6Chi monocytes during Entamoeba histolytica infection. PLoS Pathog 9:e1003096 (2013). Neutralising ; Mouse . PubMed: 23300453
    • Goren I  et al. Systemic anti-TNFalpha treatment restores diabetes-impaired skin repair in ob/ob mice by inactivation of macrophages. J Invest Dermatol 127:2259-67 (2007). PubMed: 17460730
    • Goren I  et al. Severely impaired insulin signaling in chronic wounds of diabetic ob/ob mice: a potential role of tumor necrosis factor-alpha. Am J Pathol 168:765-77 (2006). PubMed: 16507892
    • Echtenacher B  et al. Requirement of endogenous tumor necrosis factor/cachectin for recovery from experimental peritonitis. J Immunol 145:3762-6 (1990). PubMed: 2246512

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    rat, neutalization assay
    send Abtrial Info

    Read More
    Answer

    Thank you for contacting us.

    Here is the information about our Abtrial program.

    For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

    The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the to-be-tested product at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount in the amount of the price you paid for the to-be-tested product. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.


    I hope this information is helpful to you. Please let me know if you like to try one of the antibodies in rat and I can send you the discount code.

    Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com