AB269772

Anti-TNF alpha 抗体 [TNFA/1172] - BSA and Azide free

Name: Anti-TNF alpha 抗体 [TNFA/1172] - BSA and Azide free (ab269772) | Abcam
Brand: Abcam Limited
SKU: AB269772
Price: 129000 JPY
Availability: InStock

Anti-TNF alpha antibody [TNFA/1172] - BSA and Azide free

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(4 Publications)

Mouse Monoclonal TNF alpha antibody. Carrier free. Suitable for IHC-P and reacts with Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TNF.

別名を表示する

TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [TNFA/1172] - BSA and Azide free (AB269772)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [TNFA/1172] - BSA and Azide free (AB269772)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human Erdheim Chester disease tissue labeling TNF alpha with ab220210 at 4 μg/mL.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220210)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [TNFA/1172] - BSA and Azide free (AB269772)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat stomach tissue labeling TNF alpha with ab220210 at 4 μg/mL.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220210)

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

TNFA/1172

アイソタイプ

IgM

軽鎖のタイプ

kappa

キャリアフリー

Yes

交差種

Rat, Human

アプリケーション

IHC-P

applications

免疫原

Recombinant Full Length Protein corresponding to Human TNF.

P01375

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-4 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-4 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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製品の詳細

ab269772 is a carrier free version of ab220210.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A/G

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed : 23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed : 16829952, PubMed : 22517918, PubMed : 23396208). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (PubMed : 12794819). Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity).. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

See full target information TNF

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:13264 PubMed40246912

2025

Immune response against antibiotic-resistant and antibiotic-sensitive staphylococcus aureus in a rat model of implant infection.

Applications

Unspecified application

Species

Unspecified reactive species

Yingfang Fan,Amita Sekar,Madeline McCanne,Jean Yuh,Devika Dutta Kannambadi,Sashank Lekkala,Orhun K Muratoglu,Ebru Oral

Animal models and experimental medicine 7:145-155 PubMed38525803

2024

The efficacy of vitamin E in preventing arthrofibrosis after joint replacement.

Applications

Unspecified application

Species

Unspecified reactive species

Yingfang Fan,Jean Yuh,Sashank Lekkala,Mehmet D Asik,Andrew Thomson,Madeline McCanne,Mark A Randolph,Antonia F Chen,Ebru Oral

Journal of orthopaedic research : official publication of the Orthopaedic Research Society 42:460-473 PubMed37609941

2023

The efficacy of antibiotic-eluting material in a two-stage model of periprosthetic joint infection.

Applications

Unspecified application

Species

Unspecified reactive species

Yingfang Fan,Madeline McCanne,Jean Yuh,Sashank Lekkala,Charlotte P Leape,Shannon Hugard,Andrew Thomson,Jamie E Collins,Orhun K Muratoglu,Mark Randolph,Ebru Oral

Molecules (Basel, Switzerland) 28: PubMed36903410

2023

Effects of Oil on Sodium Hypochlorite-Induced Damage in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Güneş Bolatli,Fatih Taş,Naci Ömer Alayunt

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TNF alpha 抗体 [TNFA/1172] - BSA and Azide free

Anti-TNF alpha antibody [TNFA/1172] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [TNFA/1172] - BSA and Azide free (AB269772)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [TNFA/1172] - BSA and Azide free (AB269772)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

軽鎖のタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (4)

Abcam product promise