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AB325267

Anti-TNF-alpha antibody [EPR30726-11] - BSA and Azide free (Capture)

Anti-TNF-alpha antibody [EPR30726-11] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal TNF alpha antibody. Carrier free. Suitable for sELISA and reacts with Pig samples.

別名を表示する

TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

2 Images
Sandwich ELISA - Anti-TNF-alpha antibody [EPR30726-11] - BSA and Azide free (Capture) (AB325267)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-TNF-alpha antibody [EPR30726-11] - BSA and Azide free (Capture) (AB325267)

Sandwich ELISA using TNF-alpha immunogen protein standard curve with the capture antibody ab325267 dilution at 2 µg/mL and detector antibody ab325268 dilution at 0.5 µg/mL. The background-subtracted absorbance data are presented as mean ± standard deviation).

Sandwich ELISA - Anti-TNF-alpha antibody [EPR30726-11] - BSA and Azide free (Capture) (AB325267)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-TNF-alpha antibody [EPR30726-11] - BSA and Azide free (Capture) (AB325267)

Interpolated concentration of TNF-alpha was measured in duplicate at different sample concentrations. Undiluted samples are as follows : starting concentration of the first point of recovery in 100% dilution of Pig Serum and Plasma (NaCit). The interpolated dilution factor corrected values are plotted (mean +/- standard deviation, n=2). Sample dilutions are made in 1% Bovine Serum Albumin (BSA). We do not guarantee same sensitivity level due to biological variation.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR30726-11

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Pig

アプリケーション

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

The clone is specific to Pig TNF-alpha.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Pig": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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製品の詳細

ab325267 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Pig TNF-alpha. The recommended pair for sandwich ELISA is:
Capture: ab325267, Pig TNF-alpha Capture Antibody (unconjugated)
Detector: ab325268, Pig TNF-alpha Detector Antibody (unconjugated)
The reference range value is 0.98 pg/ml-62.50 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity).. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
See full target information TNF
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Abcam product promise

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