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AB9739

Anti-TNF alpha 抗体

Anti-TNF alpha antibody

4

(11 Reviews)

|

(122 Publications)

Rabbit Polyclonal TNF alpha antibody. Suitable for WB, sELISA and reacts with Recombinant fragment - Mouse samples. Cited in 122 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Tnf aa 50 to C-terminus.

別名を表示する

Tnfa, Tnfsf2, Tnf, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

2 Images
Sandwich ELISA - Anti-TNF alpha antibody (AB9739)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-TNF alpha antibody (AB9739)

ELISA analysis of ab9739. This antigen affinity purified antibody, in conjunction with a suitable detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine TNF alpha.

Western blot - Anti-TNF alpha antibody (AB9739)
  • WB

Supplier Data

Western blot - Anti-TNF alpha antibody (AB9739)

To detect mouse TNF alpha by Western Blot analysis, ab9739 can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for recombinant mouse TNF alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Lanes 1-11 : 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant mouse TNF alpha, respectively.

Non-reducing conditions.

All lanes:

Western blot - Anti-TNF alpha antibody (ab9739)

Predicted band size: 25 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

Unknown

軽鎖のタイプ

unknown

キャリアフリー

No

アプリケーション

WB, sELISA

applications

免疫原

Recombinant Fragment Protein within Mouse Tnf aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P06804

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>To detect TNF alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant TNF alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. The presursor is ~26 kDa and the secreted form is ~17 kDa.</p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p>To detect Murine TNF alpha by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9739 is required. This antigen affinity purified antibody, in conjunction with ab245857 detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine TNF alpha.</p>" } } }

製品の詳細

This product is no longer batch tested in IHC, for an IHC validated antibody please see ab1793.

出荷温度及び保存条件

製品の状態
Lyophilized
再構成
reconstitute with water at 200µL
精製方法
Affinity purification Immunogen
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

ターゲットの情報

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (PubMed : 25586176). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (PubMed : 32741026).. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
See full target information Tnf

文献 (122)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:366 PubMed40128893

2025

Mfn2 regulates calcium homeostasis and suppresses PASMCs proliferation via interaction with IP3R3 to mitigate pulmonary arterial hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Wang,Jie Wang,Jing Yu,Zhiqiang Li,Minfang Zhang,Yuhu Chen,Fen Liu,Dongmei Jiang,Jingfei Guo,Xiaomei Li,Yun Wu

The journal of headache and pain 26:55 PubMed40087597

2025

Activation of central and peripheral transient receptor potential melastatin 8 increases susceptibility to spreading depolarization and facilitates trigeminal neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Tzu-Ting Liu,Pin-Yu Chen,Chyun-Yea Tseng,Yun-Ning Chen,Jian-Bang Chen,Tz-Han Ni,Shuu-Jiun Wang,Shih-Pin Chen,Jiin-Cherng Yen

Journal of cellular and molecular medicine 28:e70084 PubMed39400399

2024

AAV9-mediated CIRP gene transfer protects against cardiac dysfunction and remodelling in a rat model of myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Zhong,Shuang Yang,Can Fang,Yanjun Li,Siwei Song,Minxiao Chen,Jingru Chen

BMC pulmonary medicine 24:477 PubMed39334020

2024

Mitochondrial transplantation attenuates lipopolysaccharide-induced acute respiratory distress syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Seo-Eun Lee,In-Hyeon Kim,Young Cheol Kang,Yujin Kim,Shin-Hye Yu,Jeong Seon Yeo,Iksun Kwon,Jun Hyeok Lim,Je-Hein Kim,Kyuboem Han,Sung-Hwan Kim,Chun-Hyung Kim

International journal of stem cells 17:284-297 PubMed38604748

2024

Impaired Osteogenesis in Human Induced Pluripotent Stem Cells with Acetaldehyde Dehydrogenase 2 Mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Jooyoung Lim,Heeju Han,Se In Jung,Yeri Alice Rim,Ji Hyeon Ju

Translational neurodegeneration 12:50 PubMed37946307

2023

Intranasal administration of induced pluripotent stem cell-derived cortical neural stem cell-secretome as a treatment option for Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hyunkyung Mo,Juryun Kim,Jennifer Yejean Kim,Jang Woon Kim,Heeju Han,Si Hwa Choi,Yeri Alice Rim,Ji Hyeon Ju

Journal of neuroinflammation 20:203 PubMed37674228

2023

RGS5 augments astrocyte activation and facilitates neuroinflammation via TNF signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shu Yin,Xin-Yue Ma,Ying-Feng Sun,Yan-Qing Yin,Ying Long,Chun-Lai Zhao,Jun-Wei Ma,Sen Li,Yan Hu,Ming-Tao Li,Gang Hu,Jia-Wei Zhou

ACS pharmacology & translational science 6:1015-1027 PubMed37470022

2023

Dasatinib Ointment Promotes Healing of Murine Excisional Skin Wound.

Applications

Unspecified application

Species

Unspecified reactive species

Surasak Wichaiyo,Saovaros Svasti,Arnatchai Maiuthed,Pattarawit Rukthong,Arman Syah Goli,Noppawan Phumala Morales

Cancers 15: PubMed37345018

2023

Synthesis of New Chromene Derivatives Targeting Triple-Negative Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Aysha Alneyadi,Zohra Nausheen Nizami,Hanan E Aburawi,Soleiman Hisaindee,Muhammad Nawaz,Samir Attoub,Gaber Ramadan,Nehla Benhalilou,Mazoun Al Azzani,Yassine Elmahi,Aysha Almeqbali,Khalid Muhammad,Ali H Eid,Ranjit Vijayan,Rabah Iratni

International journal of molecular sciences 24: PubMed36768965

2023

Investigating Therapeutic Effects of Indole Derivatives Targeting Inflammation and Oxidative Stress in Neurotoxin-Induced Cell and Mouse Models of Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Jen Chiu,Chih-Hsin Lin,Chung-Yin Lin,Pei-Ning Yang,Yen-Shi Lo,Yu-Chieh Chen,Chiung-Mei Chen,Yih-Ru Wu,Ching-Fa Yao,Kuo-Hsuan Chang,Guey-Jen Lee-Chen
View all publications

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