Anti-TNF alpha 抗体 (ab6671)
Key features and details
- Rabbit polyclonal to TNF alpha
- Suitable for: ELISA, IHC-P, ICC/IF, WB, Neutralising
- Reacts with: Human, Cynomolgus monkey, Recombinant fragment
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-TNF alpha antibody
TNF alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TNF alpha -
由来種
Rabbit -
特異性
The immunogen used to raise this antibody shares 78% homology with mouse and rat TNF alpha protein. Some customers have successfully used ab6671 with mouse and rat samples, however we do not batch test in these species and therefore we cannot guarantee this product will consistently work with mouse and rat. We would recommend ab205587 or ab1793 as an alternative product for use with these species.
The antibody does not recognize human TNF beta (lymphotoxin).
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アプリケーション
適用あり: ELISA, IHC-P, ICC/IF, WB, Neutralisingmore details -
種交差性
交差種: Human, Cynomolgus monkey, Recombinant fragment
交差が予測される動物種: Mouse, Rat, Dog, Pig, Monkey, Non human primates
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免疫原
Recombinant full length protein corresponding to Human TNF alpha.
Database link: P01375 -
ポジティブ･コントロール
- WB: Recombinant TNF-alpha protein.
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特記事項
Endotoxin content by LAL is <10 pg/ml.
Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077). Or search our wide range of secondary antibodies for use with your experiment.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
特記事項（精製）
This antibody is primarily directed against mature 17,000 MW human TNF alpha and is useful in determining its presence in various assays. In general, this antibody also detects primate TNF alpha in the same formats using similar dilutions. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Affibody® Molecule
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6671の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
1/1000 - 1/5000.
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| IHC-P | (19) |
1/100 - 1/500.
|
| ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 19458984
|
| WB | (10) |
1/500 - 1/2000. Predicted molecular weight: 25.6 kDa.
Membrane Blocking is recommended with BSA not Milk for this product. Suitable for use as a positive control for Western blot against recombinant TNFalpha produced in E.Coli. |
| Neutralising |
Use at an assay dependent concentration.
|
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| IF |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
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ELISA
1/1000 - 1/5000. |
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IHC-P
1/100 - 1/500. |
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ICC/IF
Use at an assay dependent concentration. PubMed: 19458984 |
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WB
1/500 - 1/2000. Predicted molecular weight: 25.6 kDa. Membrane Blocking is recommended with BSA not Milk for this product. Suitable for use as a positive control for Western blot against recombinant TNFalpha produced in E.Coli. |
|
Neutralising
Use at an assay dependent concentration. |
|
IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. -
関連疾患
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
配列類似性
Belongs to the tumor necrosis factor family. -
翻訳後修飾
The soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7124 Human
- Entrez Gene: 21926 Mouse
- Entrez Gene: 24835 Rat
- Omim: 191160 Human
- SwissProt: P79337 Cynomolgus monkey
- SwissProt: P51742 Dog
- SwissProt: P01375 Human
- SwissProt: P06804 Mouse
see all -
別名
- APC1 antibody
- APC1 protein antibody
- Cachectin antibody
see all
画像
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Western blot - Anti-TNF alpha antibody (ab6671)Anti-TNF alpha antibody (ab6671) at 1/1000 dilution + Recombinant TNF alpha protein
Secondary
Goat Anti-Rabbit Dylight® 649 Conjugate at 1/20000 dilution
Predicted band size: 25.6 kDa
Additional bands at: 16 kDa (possible cleavage fragment)Western Blot analysis, labeling TNF alpha with ab6671 at 1/1000. Blocking was with 1% BSA in TBS-T incubated for 30 minutes at room temperature. The cleavage fragment is related to the soluble isoform 77 - 223.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (846)
ab6671 は 846 報の論文で使用されています。
- Lai C et al. Synovial fibroblast-miR-214-3p-derived exosomes inhibit inflammation and degeneration of cartilage tissues of osteoarthritis rats. Mol Cell Biochem 478:637-649 (2023). PubMed: 36001206
- Yalcin A et al. Protective effect of melatonin on learning and memory impairment and hippocampal dysfunction in rats induced by high-fructose corn syrup. Iran J Basic Med Sci 26:69-75 (2023). PubMed: 36594054
- D'Onofrio N et al. SIRT3 mediates the effects of PCSK9 inhibitors on inflammation, autophagy, and oxidative stress in endothelial cells. Theranostics 13:531-542 (2023). PubMed: 36632236
- Thangavel H et al. Susceptibility of Fat Tissue to SARS-CoV-2 Infection in Female hACE2 Mouse Model. Int J Mol Sci 24:N/A (2023). PubMed: 36674830
- Thangavel H et al. Diets Differently Regulate Tumorigenesis in Young E0771 Syngeneic Breast Cancer Mouse Model. J Clin Med 12:N/A (2023). PubMed: 36675341
