AB6671

Anti-TNF alpha 抗体

Name: Anti-TNF alpha 抗体 (ab6671) | Abcam
Brand: Abcam Limited
SKU: AB6671
Price: 57000 JPY
Availability: InStock
Rating: 4 (35 reviews)

Anti-TNF alpha antibody

Lab Essentials
詳細を見る

(35 Reviews)

(1021 Publications)

Anti-TNF alpha antibody (ab6671) is a rabbit polyclonal antibody detecting TNF alpha in Western Blot, IHC-P, ICC/IF, ELISA, Neut. Suitable for Cynomolgus monkey, Human,.

- Over 840 publications
- Trusted since 2002

別名を表示する

TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

3 Images

Supplier Data

Western blot - Anti-TNF alpha antibody (AB6671)

Western Blot analysis, labeling TNF alpha with ab6671 at 1/1000. Blocking was with 1% BSA in TBS-T incubated for 30 minutes at room temperature. The cleavage fragment is related to the soluble isoform 77 - 223.

All lanes:

Western blot - Anti-TNF alpha antibody (ab6671) at 1/1000 dilution

All lanes:

Recombinant TNF alpha protein

Secondary

All lanes:

Goat Anti-Rabbit Dylight® 649 Conjugate at 1/20000 dilution

Predicted band size: 25 kDa

Observed band size: 16 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody (AB6671)

IHC-P

CiteAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody (AB6671)

TNF alpha immunohistochemistry using Anti-TNF alpha antibody ab6671. Publication image and figure legend from Manetti, M., Linari, S., et al. 2019, J Clin Med, PubMed : 31261789.

Expression of tumor necrosis factor (TNF)-α, TNF receptor 1 (TNF-R1), and TNF receptor 2 (TNF-R2) in synovial tissue from patients with hemophilic arthropathy (HA) and osteoarthritis (OA). (A) Representative microphotographs of tissue sections subjected to immunoperoxidase-based immunohistochemical staining for TNF-α (brown color), TNF-R1, and TNF-R2 (purple color) are shown. TNF-α, TNF-R1, and TNF-R2 immunostaining is strongly increased either in the hyperplastic lining or in the sublining layers of the HA synovium compared to non-inflamed OA control synovium. Original magnification : x10 and x20. Scale bar : 200 µm (x10 panels), 100 µm (x20 panels). (B) Western blotting of total protein extracts from the synovium of HA patients (n = 10) and OA controls (n = 8). Representative immunoblots for TNF-α, TNF-R1, and TNF-R2 are shown. The densitometric analysis of the bands normalized to α-tubulin is reported in the histograms. Data are the mean ± SEM of the optical density in arbitrary units (a.u.). * p < < 0.05 vs. OA (Student's t-test).

CiteAb

Western blot - Anti-TNF alpha antibody (AB6671)

TNF western blot using Anti-TNF alpha antibody ab6671. Publication image and figure legend from Manetti, M., Linari, S., et al., 2019, J Clin Med, PubMed 31261789.

Fibroblast-like synoviocytes (FLS) from patients with hemophilic arthropathy (HA-FLS) overexpress tumor necrosis factor (TNF)-α, TNF receptor 1 (TNF-R1), and TNF receptor 2 (TNF-R2) and effectively proliferate in response to TNF-α. (A) Western blotting of total protein extracts from cultured HA-FLS and osteoarthritis control FLS (OA-FLS). Representative TNF-α, TNF-R1, and TNF-R2 immunoblots are shown; α-tubulin was used as a loading control. The densitometric analysis of the bands normalized to α-tubulin is reported in the histograms. Data are mean ± SEM of optical density in arbitrary units (a.u.). * p < < 0.05 vs. OA-FLS (Student's t-test). (B) Cell viability evaluated at the basal condition or after treatment with recombinant human TNF-α (10 ng/mL) using the water-soluble tetrazolium (WST)-1 cell proliferation reagent. Cell viability in response to TNF-α is expressed as the percentage increase/decrease over the basal response for both HA-FLS and OA-FLS. Bars represent the mean ± SEM. Results are representative of three independent experiments performed with each one of the six HA-FLS and six OA-FLS lines. * p < < 0.05 vs. the respective basal condition (Student's t-test). (C) Levels of active (cleaved) caspase-3 in HA-FLS and OA-FLS, as measured by the specific enzyme-linked immunosorbent assay on cell lysates. Data are the mean ± SEM of three independent experiments performed in triplicate with each one of the six HA-FLS and six OA-FLS lines.

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human, Cynomolgus monkey

アプリケーション

IF, ICC/IF, ELISA, WB, IHC-P

applications

免疫原

Recombinant Full Length Protein corresponding to Human TNF.

P01375

特異性

The immunogen used to raise this antibody shares 78% homology with mouse and rat TNF alpha protein. Some customers have successfully used ab6671 with mouse and rat samples, however we do not batch test in these species and therefore we cannot guarantee this product will consistently work with mouse and rat. We would recommend ab205587 or ab1793 as an alternative product for use with these species.The antibody does not recognize human TNF beta (lymphotoxin).

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