AB66579

Anti-TNF alpha 抗体

Name: Anti-TNF alpha 抗体 (ab66579) | Abcam
Brand: Abcam Limited
SKU: AB66579
Price: 57000 JPY
Availability: InStock
Rating: 4 (10 reviews)

Anti-TNF alpha antibody

Lab Essentials
詳細を見る

(10 Reviews)

(179 Publications)

Anti-TNF alpha antibody (ab66579) is a rabbit polyclonal antibody detecting TNF alpha in Western Blot, ICC/IF. Suitable for Human, Mouse.

- Over 140 publications
- Trusted since 2009

別名を表示する

TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

2 Images

Lab

Western blot - Anti-TNF alpha antibody (AB66579)

Mature THP-1 and Raw264.7 cells were treated with LPS (1 ug/ml) for 6 hours to stimulate production of TNF alpha. Brefeldin A (300 ng/ml) was added in the final 3 hours of incubation to inhibit golgi traffic.

All lanes:

Western blot - Anti-TNF alpha antibody (ab66579) at 1 µg/mL

Lane 1:

THP1 cells treated with PMA (negative control) at 20 µg

Lane 2:

THP1 cells treated with PMA+LPS+Brefeldin A at 20 µg

Lane 3:

Raw264.7 cells treated with PMA (negative control) at 20 µg

Lane 4:

Raw264.7 cells treated with PMA+LPS+Brefeldin A at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 25 kDa

Observed band size: 26 kDa,28 kDa,50 kDa,75 kDa

false

Exposure time: 2min

Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody (AB66579)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody (AB66579)

ab66579 staining TNF alpha in Raw264.7 cells. The cells were treated with LPS for 7 hours and Brefeldin A (ab120299) for the final 3 hours. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab66579 at 1μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 4% paraformaldehyde (10 min).

Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

Ab66579 has been batch tested in Western Blot using LPS stimulated lysates. Abcam does not recommend this product for use in IHC. Please contact Abcam Scientific Support for more information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "Abcam recommends using 3% Milk as the blocking agent.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "Abcam recommends using 3% Milk as the blocking agent.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Baboon": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-TNF alpha antibody (ab66579) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

What is the molecular weight of TNF alpha?
Anti-TNF alpha (ab66579) specifically detects a band for TNF alpha (UniProt: P01375) at a molecular weight of 25kDa.

Trusted by the scientific community
Anti-TNF alpha (ab66579) was first used in a scientific publication in 2009 and has been cited over 140 times in peer-reviewed journals.

Reviewed by scientists
Anti-TNF alpha (ab66579) has over 10 independent reviews from customers.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed : 23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed : 16829952, PubMed : 22517918, PubMed : 23396208). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (PubMed : 12794819). Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity).. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

See full target information TNF

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文献 (179)

Recent publications for all applications. Explore the full list and refine your search

Parasitology research 124:98 PubMed40906224

2025

The potential relationship between Toxocara canis infection and epilepsy in a rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Amina M Salama,Dina I Elgendy,Rasha A Elmahy,Asmaa F Eltantawy,Monira A Seleem,Ahmed M Elgohary,Omaima K Docmac,Marwa E F Al Mwafy,Ahmed A Almeldin,Mahmoud S Sharaf

Eye and vision (London, England) 12:36 PubMed40898311

2025

Early retinal changes in type 2 diabetes detected by texture-based OCT analysis: potential approach for subclinical diabetic retinopathy diagnosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Oliveira,Pedro Guimarães,Ângelo Roque-Rosado,Elisa Julião Campos,Pedro Serranho,Paulo Matafome,Rui Bernardes,António Francisco Ambrósio

The Korean journal of pain 38:292-307 PubMed40556327

2025

MiR-298 suppresses astrocytic NF-κB activity and neuroinflammation targeting MyD88 in bone cancer pain.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Liu,Denggui Wang,Zhirong Yan,Min Zhou

Frontiers in immunology 16:1536056 PubMed40453075

2025

Transcutaneous auricular vagus nerve stimulation alleviates inflammation-induced depression by modulating peripheral-central inflammatory cytokines and the NF-κB pathway in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xingke Song,Haohan Zhu,Zijie Chen,Yifei Wang,Jinling Zhang,Yu Wang,Peijing Rong,Junying Wang

Molecular neurobiology 62:8261-8278 PubMed39994158

2025

Caloric Restriction Attenuated Nerve Damages Mediated Through SIRT-1-a Study Using Nerve Crush Injury Model in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Meei-Ling Sheu,Liang-Yi Pan,Szu-Yen Pan,Ying Ju Chen,Jason Sheehan,Weir-Chiang You,Chien-Chia Wang,Hung-Chuan Pan

Investigative ophthalmology & visual science 66:7 PubMed39760689

2025

Retinal OCT-Derived Texture Features as Potential Biomarkers for Early Diagnosis and Progression of Diabetic Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Oliveira,Pedro Guimarães,Elisa Julião Campos,Rosa Fernandes,João Martins,Miguel Castelo-Branco,Pedro Serranho,Paulo Matafome,Rui Bernardes,António Francisco Ambrósio

The Journal of biological chemistry 301:108137 PubMed39730060

2024

DNMT3b-mediated CpA methylation facilitates REST binding and gene silencing and exacerbates hippocampal demyelination in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tie-Feng Yao,Zhi-Yun Wang,Lu Sun,Sheng-Xue Yu,Hong-Dan Yu,Zheng-Zhong Yang,Wan-Ze Li,Lin Niu,Die Sun,Ya-Hui Shi,Jun-Qi Li,Wen-Qiang Liu,Xue-Zheng Liu,Zhong-Fu Zuo

International journal of ophthalmology 17:2158-2166 PubMed39697880

2024

Alkali burn injury model of meibomian gland dysfunction in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Li,Ya-Qiong Yang,Yong Lin,Ke Yan,Yu-Fei Lyu,Zhao-Qiang Zhang,Cai-Hong Huang,Jiao-Yue Hu,Zu-Guo Liu

Frontiers in pain research (Lausanne, Switzerland) 5:1488369 PubMed39654799

2024

Paclitaxel triggers molecular and cellular changes in the choroid plexus.

Applications

Unspecified application

Species

Unspecified reactive species

Alemeh Zamani,Parisa EmamiAref,Lucie Kubíčková,Klaudia Hašanová,Ondřej Šandor,Petr Dubový,Marek Joukal

Antioxidants (Basel, Switzerland) 13: PubMed39334796

2024

Comparative Study on Hepatoprotective Effects of Traditional Herbs, Roots of Nakai, Fischer, Mill., and Fruits of Pall., on Ethanol-Induced Liver Injury in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

So-Yeon Kim,Kyung-Jin Oh,Yu-Ri Seo,Young-Woo Kim,Phil Hyun Song,Chang-Hyun Song

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TNF alpha 抗体

Anti-TNF alpha antibody

Western blot - Anti-TNF alpha antibody (AB66579)

Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody (AB66579)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (179)

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