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AB1793

Anti-TNF alpha 抗体 [52B83]

Anti-TNF alpha antibody [52B83]

4

(19 Reviews)

|

(378 Publications)

Anti-TNF alpha antibody [52B83] (ab1793) is a mouse monoclonal antibody detecting TNF alpha in Western Blot, Flow Cytometry (Intra), IHC-Fr, ICC/IF, ELISA. Suitable for Guinea pig, Human, Mouse, Rhesus monkey.

- Over 270 publications
- Trusted since 2002

別名を表示する

TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (AB1793)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (AB1793)

ab1793 staining TNFα in RAW 264.7 cells treated with (R,S)-rolipram (ab120029), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (R,S)-rolipram, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120029 ((R,S)-rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight® 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Flow Cytometry (Intracellular) - Anti-TNF alpha antibody [52B83] (AB1793)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TNF alpha antibody [52B83] (AB1793)

Overlay histogram showing RAW 264.7 cells stained with ab1793 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1793, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in RAW 264.7 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (AB1793)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (AB1793)

ab1793 staining TNFα in RAW 264.7 cells treated with (R)-(-)-rolipram (ab120031), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (R)-(-)-rolipram , as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120031 ((R)-(-)--rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight® 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (AB1793)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (AB1793)

ab1793 staining TNFα in RAW 264.7 cells treated with (S)-(+)-rolipram (ab120030), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (S)-(+)-rolipram , as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120030 ((S)-(+)-rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793(5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight® 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Immunohistochemistry - Anti-TNF alpha antibody [52B83] (AB1793)
  • IHC

CiteAb

Immunohistochemistry - Anti-TNF alpha antibody [52B83] (AB1793)

Immunohistochemistry using Anti-TNF alpha antibody [52B83], ab1793. Publication image from Yu, M. et al., 2015, Nat Commun, 25565658. Legend direct from paper.

Reduced WAT inflammatory response in ob/ob/Fsp27−/− mice.Four-month-old chow-fed ob/ob and ob/ob/Fsp27−/− mice were used for the analyses in (a–i). (a) Gene expression profiling in the GWAT of ob/ob and ob/ob/Fsp27−/− mice. Green circles represent downregulated genes in the ob/ob/Fsp27−/− mice compared with ob/ob mice. The intensity of the green colour indicates the extent of downregulation. (b) Relative mRNA expression of F4/80, CD11c and other pro-inflammatory genes, or (c) anti-inflammatory genes in the GWAT of ob/ob and ob/ob/Fsp27−/− mice (n=4 per group). (d) F4/80 immunohistochemical analysis in the GWAT of ob/ob and ob/ob/Fsp27−/− mice. Scale bar, 64 µm. (e) Serum concentration of IL-6 (n=7 per group). (f) Serum concentration of TNFα (n=7 for ob/ob and n=9 for ob/ob/Fsp27−/−). (g) Serum concentration of adiponectin (n=8 per group). (h) TNFα immunohistochemical analysis in the GWAT of ob/ob and ob/ob/Fsp27−/− mice showing the CLSs (left). Right : statistic analysis of the CLSs per 1,000 adipocytes. Scale bar, 50 µm. (i) Relative mRNA levels of ASC, NLRP3, Caspase-1 and TXNIP in the GWAT of ob/ob and ob/ob/Fsp27−/− mice (n=3 per group). Quantitative data are presented as mean±s.e.m. Significance was established using a two-tailed Student’s t-test. Differences were considered significant at P<0.05. *P<0.05, **P<0.01, ***P<0.001.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

52B83

アイソタイプ

IgG1

軽鎖のタイプ

unknown

キャリアフリー

No

交差種

Mouse, Rhesus monkey, Guinea pig, Human

アプリケーション

ELISA, WB, ICC/IF, Flow Cyt (Intra)

applications

免疫原

Native Full Length Protein corresponding to Human TNF.

P01375

特異性

<p>This antibody detects both natural and recombinant TNFa. It does not cross-react with TNF beta or lymphotoxin. It reacts with free soluble (17 kDa) and membrane (26 kDa) human TNF-alpha. It does not react with receptor-bound TNF-alpha. Non-specific background staining is observed in connective tissues.</p>

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代替製品

Primary Antibodies

AB215188

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AB222500

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AB225576

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Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [EPR20972] - BSA and Azide free (AB225576)

  • 0
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Primary Antibodies

AB237355

PE Anti-TNF alpha antibody [EPR20972]

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Flow Cytometry (Intracellular) - PE Anti-TNF alpha antibody [EPR20972] (AB237355)

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AB66579

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AB183218

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組合せ製品

Assay Kits

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Human TNF alpha ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

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Primary Antibodies

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Immunocytochemistry - Anti-IL-1 beta antibody [RM1009] (AB283818)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Primary Antibodies

AB233706

Anti-IL-6 antibody [EPR21711]

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p>TNF-alpha is normally secreted as a homotrimer with a molecular mass of 52 kDa. Monomeric TNF-alpha is 17.4 kDa A reduced sample treatment and 15% SDS-Page was used.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p>(methanol fixed cells)</p><p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "2 µg/mL", "IHCFr-species-notes": "<p>Fixed in acetone for 10 minutes</p>" }, "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p>TNF-alpha is normally secreted as a homotrimer with a molecular mass of 52 kDa. Monomeric TNF-alpha is 17.4 kDa A reduced sample treatment and 15% SDS-Page was used.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10", "FlowCytIntra-species-notes": "<p>(methanol fixed cells)</p><p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "2 µg/mL", "IHCFr-species-notes": "<p></p>" }, "Guinea pig": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p>TNF-alpha is normally secreted as a homotrimer with a molecular mass of 52 kDa. Monomeric TNF-alpha is 17.4 kDa A reduced sample treatment and 15% SDS-Page was used.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p>(methanol fixed cells)</p><p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "2 µg/mL", "IHCFr-species-notes": "<p></p>" }, "Rhesus monkey": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p>TNF-alpha is normally secreted as a homotrimer with a molecular mass of 52 kDa. Monomeric TNF-alpha is 17.4 kDa A reduced sample treatment and 15% SDS-Page was used.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p>(methanol fixed cells)</p><p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "2 µg/mL", "IHCFr-species-notes": "<p></p>" } } }
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製品の詳細

Product Specifications
Anti-TNF alpha antibody [52B83] (ab1793) is a mouse monoclonal antibody and is validated for use in ELISA, Flow Cyt (Intra), ICC/IF, IHC-Fr, WB in guineapig, human, mouse, rhesus monkey samples.
Anti-TNF alpha antibody [52B83] (ab1793) specifically detects TNF alpha (UniProt ID: P01375; Molecular weight: 26kDa) and is sold in 50 µg selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-TNF alpha antibody [52B83] (ab1793) has high sensitivity and specificity.
Anti-TNF alpha antibody [52B83] (ab1793) has been cited over 275 times in peer reviewed journals and is trusted by the scientific community.
Anti-TNF alpha antibody [52B83] (ab1793) has 16 independent reviews from customers.

Related Products
Conjugation-ready, carrier free format available for antibody clone 52B83 - ab233027.
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein G
バッファー組成
Preservative: 0.02% Sodium azide Constituents: PBS, 0.1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed : 23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed : 16829952, PubMed : 22517918, PubMed : 23396208). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (PubMed : 12794819). Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity).. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
See full target information TNF
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文献 (378)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastroenterology 31:104277 PubMed40900774

2025

Sex based relative expression of estrogen receptors and tumor necrosis factor-alpha in liver affects hepatitis C virus viral pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Groover,Sarah Addison,Savannah Nicks,Mitchelle Mwangi,Amy Brooks,Anil Kaul,Rashmi Kaul

Journal of nanobiotechnology 23:559 PubMed40790215

2025

Dynamic three-dimensional culture enhances tunneling nanotubes-mediated mitochondrial transfer in mesenchymal stromal cells to accelerate wound healing.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Ma,Xiaoxue Yang,Xiaoyao Huang,Hao Guo,Zihan Li,Siyuan Fan,Han Qin,Fanhui Meng,Peisheng Liu,Xinyu Wang,Meiling Wu,Kun Xuan,Anqi Liu

Frontiers in endocrinology 16:1620032 PubMed40747306

2025

Bioinformatic analysis identifies LPL as a critical gene in diabetic kidney disease via lipoprotein metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Dong,Huan Xu,Pengjie Xu,Jiang Liu,Zhouji Shen

Science advances 11:eadv4238 PubMed40712009

2025

Genetically engineered chondrocyte-mimetic nanoplatform attenuates osteoarthritis by blocking IL-1β and restoring sirtuin-3.

Applications

Unspecified application

Species

Unspecified reactive species

Caifeng Deng,Liukang Yu,Xuan Zhao,Yuxiao Chen,Jiabin Mei,Jie Wei,Xiaoyuan Chen,Guanghua Lei,Chao Zeng

Frontiers in medicine 12:1569590 PubMed40630478

2025

Network pharmacology-based approach to research the effects and mechanisms of injection against idiopathic pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Liangyu Chen,Haobo Lin,Linmang Qin,Guangfeng Zhang,Peisheng Chen,Zebo Jiang,Pan Xu,Donghui Huang,Xiao Zhang

BMC biology 23:176 PubMed40598379

2025

Astaxanthin prevents postovulatory oocyte aging by targeting TNFR2 and inhibiting the TNF signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Wen,Zhe Han,Qi Yang,Ke-Song Shi,Dui Sun,Xiao-Jie Zhang,Cheng-Guang Liang

Marine drugs 23: PubMed40559659

2025

Rhamnan Sulfate from the Seaweed May Improve Cognitive Impairment Caused by Hyperglycemia.

Applications

Unspecified application

Species

Unspecified reactive species

Takaichi Miwa,Masaya Sato,Ning Ma,Keiichi Hiramoto,Masahiro Terasawa,Koji Suzuki

Frontiers in cellular and infection microbiology 15:1538461 PubMed40496014

2025

SARS-CoV-2 exploits steroidogenic machinery, triggers lipid metabolism for viral replication and induces immune response in Leydig cells of K18-hACE2 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Salmo Azambuja de Oliveira,André Acácio Souza da Silva,Barry T Hinton,Giovanni Freitas Gomes,Thiago Mattar Cunha,Paulo Sérgio Cerri,Estela Sasso-Cerri

Signal transduction and targeted therapy 10:182 PubMed40484878

2025

Single-cell transcriptional dissection illuminates an evolution of immunosuppressive microenvironment during pancreatic ductal adenocarcinoma metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowei Liu,Jinen Song,Meiling Yuan,Fengli Zuo,Huihui Li,Leyi Tang,Xinmin Wang,Xueyan Wang,Qian Xiao,Li Li,Xinyu Liu,Zhankun Yang,Jianlin Wu,Jing Jing,Xuelei Ma,Hubing Shi

Frontiers in immunology 16:1570692 PubMed40469287

2025

Neuroinflammation associated with proviral DNA persists in the brain of virally suppressed people with HIV.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah J Byrnes,Janna Jamal Eddine,Jingling Zhou,Emily Chalmers,Emma Wanicek,Narin Osman,Trisha A Jenkins,Michael Roche,Bruce J Brew,Jacob D Estes,Thomas A Angelovich,Melissa J Churchill
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
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