Anti-TMS1/ASC 抗体 [EPR10402(B)] (ab151700)
![Western blot - Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700)](https://www.abcam.co.jp/ps/products/151/ab151700/Images/ab151700-293447-anti-tms1-antibody-epr10402b-western-blot.jpg "Western blot - Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10402(B)] to TMS1/ASC
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-TMS1/ASC antibody [EPR10402(B)]
TMS1/ASC 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10402(B)] to TMS1/ASC -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human TMS1/ASC. The exact sequence is proprietary.
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ポジティブ･コントロール
- THP1, MCF7, U937 cell lysates; THP1 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR10402(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab151700の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
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| IP |
1/10 - 1/100.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 22 kDa. |
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IP
1/10 - 1/100. |
ターゲット情報
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機能
Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. -
組織特異性
Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells. -
配列類似性
Contains 1 CARD domain.
Contains 1 DAPIN domain. -
ドメイン
Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain. -
翻訳後修飾
Phosphorylated. -
細胞内局在
Cytoplasm. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures. - Information by UniProt
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参照データベース
- Entrez Gene: 29108 Human
- Omim: 606838 Human
- SwissProt: Q9ULZ3 Human
- Unigene: 499094 Human
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別名
- Apoptosis associated speck like protein containing a CARD antibody
- Apoptosis-associated speck-like protein containing a CARD antibody
- ASC antibody
see all
画像
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Western blot - Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700)All lanes : Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700) at 1/5000 dilution (purified)
Lane 1 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate
Lane 2 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 22 kDaBlocking and diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700)](https://www.abcam.co.jp/ps/products/151/ab151700/Images/ab151700-269770-anti-tms1-antibody-epr10402b-western-blot.jpg)
Western blot - Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: TMS1/ASC knockout HAP1 cell lysate (20 µg)
Lane 3: U937 cell lysate (20 µg)
Lane 4: THP1 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab151700 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.Unpurified ab151700 was shown to specifically react with TMS1/ASC when TMS1/ASC knockout samples were used. Wild-type and TMS1/ASC knockout samples were subjected to SDS-PAGE. Unpurified ab151700 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Immunoprecipitation - Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700)](https://www.abcam.co.jp/ps/products/151/ab151700/Images/ab151700-293460-anti-tms1-antibody-epr10402b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700)ab151700 (purified) at 1:30 dilution (2ug) immunoprecipitating TMS1/ASC in THP-1 (Human monocytic leukemia monocyte) whole cell lysate.
Lane 1 (input): THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10ug
Lane 2 (+): ab151700 & THP-1 (Human monocytic leukemia monocyte) whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab151700 in THP-1 (Human monocytic leukemia monocyte) whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
![Western blot - Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700)](https://www.abcam.co.jp/ps/products/151/ab151700/Images/ab151700-158718-anti-tms1-antibody-epr10402b-western-blot.jpg)
Western blot - Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700)All lanes : Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700) at 1/1000 dilution (unpurified)
Lane 1 : THP1 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : U937 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 22 kDa -
![Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700)](https://www.abcam.co.jp/ps/products/151/ab151700/Images/ab151700-6-benefits-of-recombinant-antibodies.png)
Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab151700 は 13 報の論文で使用されています。
- Lun P et al. HOTTIP downregulation reduces neuronal damage and microglial activation in Parkinson's disease cell and mouse models. Neural Regen Res 17:887-897 (2022). PubMed: 34472490
- Luo XY et al. miR-25-3p ameliorates SAE by targeting the TLR4/NLRP3 axis. Metab Brain Dis 37:1803-1813 (2022). PubMed: 35704145
- Xue S et al. Kui Jie Tong Ameliorates Ulcerative Colitis by Regulating Gut Microbiota and NLRP3/Caspase-1 Classical Pyroptosis Signaling Pathway. Dis Markers 2022:2782112 (2022). PubMed: 35832643
- Li H et al. Dimethyl fumarate ameliorates lipopolysaccharide-induced acute lung injury by inhibiting NLRP3 inflammasome-mediated pyroptosis through enhancing Nrf2 signaling. Toxicol Res (Camb) 11:437-450 (2022). PubMed: 35782648
- Jiang J & Cai M Cardamonin Inhibited IL-1ß Induced Injury by Inhibition of NLRP3 Inflammasome via Activating Nrf2/NQO-1 Signaling Pathway in Chondrocyte. J Microbiol Biotechnol 31:794-802 (2021). PubMed: 34024891